Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 1.14.19.53 - all-trans-retinol 3,4-desaturase and Organism(s) Homo sapiens and UniProt Accession Q4G0S4

for references in articles please use BRENDA:EC1.14.19.53
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
A cytochrome P-450 (heme thiolate) enzyme found in vertebrates. The enzyme is also active with retinal and retinoic acid.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Homo sapiens
UNIPROT: Q4G0S4
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Homo sapiens
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
+
2
reduced adrenodoxin
+
2
+
=
+
2
oxidized adrenodoxin
+
2
Synonyms
cyp27c1, cytochrome p450 27c1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cytochrome P450 27C1
-
CYP27C1
-
-
-
-
PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
all-trans-retinol,reduced adrenodoxin:oxygen 3,4-oxidoreductase
A cytochrome P-450 (heme thiolate) enzyme found in vertebrates. The enzyme is also active with retinal and retinoic acid.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
all-trans-retinal + 2 reduced adrenodoxin + 2 H+ + O2
all-trans-3,4-didehydroretinal + 2 oxidized adrenodoxin + 2 H2O
show the reaction diagram
-
-
-
?
all-trans-retinoic acid + 2 reduced adrenodoxin + 2 H+ + O2
all-trans-3,4-didehydroretinoic acid + 2 oxidized adrenodoxin + 2 H2O
show the reaction diagram
-
-
-
?
all-trans-retinol + 2 reduced adrenodoxin + 2 H+ + O2
all-trans-3,4-didehydroretinol + 2 oxidized adrenodoxin + 2 H2O
show the reaction diagram
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.00035
all-trans-retinal
pH 7.4, temperature not specified in the publication
0.00087
all-trans-retinoic acid
pH 7.4, temperature not specified in the publication
0.0005 - 0.0011
all-trans-retinol
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.004
all-trans-retinal
pH 7.4, temperature not specified in the publication
0.023
all-trans-retinoic acid
pH 7.4, temperature not specified in the publication
0.068 - 0.38
all-trans-retinol
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
61
all-trans-retinol
pH 7.4, 37°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
the most likely catalytic mechanism begins with abstraction of a hydrogen atom from C-4 (or possibly C-3) initiating the desaturation pathway, followed by a sequential abstraction of a hydrogen atom or proton-coupled electron transfer. Adrenodoxin reduction and hydrogen abstraction both contribute to rate limitation
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
C27C1_HUMAN
542
0
60920
Swiss-Prot
Mitochondrion (Reliability: 2)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kramlinger, V.; Nagy, L.; Fujiwara, R.; Johnson, K.; Phan, T.; Xiao, Y.; Enright, J.; Toomey, M.; Corbo, J.; Guengerich, F.
Human cytochrome P450 27C1 catalyzes 3,4-desaturation of retinoids
FEBS Lett.
590
1304-1312
2016
Homo sapiens (Q4G0S4), Homo sapiens
Manually annotated by BRENDA team
Johnson, K.; Phan, T.; Albertolle, M.; Peter Guengerich, F.
Human mitochondrial cytochrome P450 27C1 is localized in skin and preferentially desaturates trans-retinol to 3,4-dehydroretinol
J. Biol. Chem.
292
13672-13687
2017
Homo sapiens (Q4G0S4), Homo sapiens
Manually annotated by BRENDA team