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Information on EC 1.14.19.47 - acyl-lipid (9-3)-desaturase and Organism(s) Borago officinalis and UniProt Accession O04353

for references in articles please use BRENDA:EC1.14.19.47
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EC Tree
IUBMB Comments
The enzyme, characterized from the moss Physcomitrella patens and the plant Borago officinalis (borage), introduces a cis double bond at carbon 6 of several acyl-lipids that contain an existing Delta9 cis double bond. The enzyme contains a cytochrome b5 domain that acts as the electron donor for the active site of the desaturase.
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This record set is specific for:
Borago officinalis
UNIPROT: O04353
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Word Map
  • 1.14.19.47
  • coherence
  • retinal
  • eyes
  • macular
  • obsessive-compulsive
  • angiography
  • obsess
  • glaucoma
  • compulsive
  • spectral-domain
  • acuity
  • stratus
  • fourier-domain
  • glaucomatous
  • swept-source
  • zeiss
  • meditec
  • foveal
  • peripapillary
  • spectralis
  • cirrus
  • microperimetry
  • food industry
  • pachymetry
  • perimetry
  • intraretinal
  • agriculture
  • time-domain
  • mferg
  • dublin
  • ss-oct
  • nutrition
  • malapposition
  • optovue
  • chorioretinopathy
  • visante
  • grader
  • subfoveal
  • raster
  • parafoveal
  • strut
  • oct-based
  • cognitive-behavioral
  • hyporeflective
  • choriocapillaris
  • swept
  • topcon
  • etdrs
  • a-scans
  • heidelberg
  • rtvue
  • cystoid
  • myopic
The taxonomic range for the selected organisms is: Borago officinalis
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
pydes6, fatty acyl delta6 desaturase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
DELTA6-desaturase
-
DELTA6-fatty acid desaturase
-
acyl-lipid 6-desaturase
-
-
-
-
DELTA6-desaturase
PATHWAY SOURCE
PATHWAYS
-
-, -, -
SYSTEMATIC NAME
IUBMB Comments
DELTA9 acyl-[glycerolipid],ferrocytochrome b5:oxygen oxidoreductase (6,7-cis-dehydrogenating)
The enzyme, characterized from the moss Physcomitrella patens and the plant Borago officinalis (borage), introduces a cis double bond at carbon 6 of several acyl-lipids that contain an existing Delta9 cis double bond. The enzyme contains a cytochrome b5 domain that acts as the electron donor for the active site of the desaturase.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
stearidonoyl-[glycerolipid] + ferricytochrome b5 + H2O
show the reaction diagram
-
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
gamma-linolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
show the reaction diagram
-
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
gamma-linolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
show the reaction diagram
-
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
alpha-linolenoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
stearidonoyl-[glycerolipid] + ferricytochrome b5 + H2O
show the reaction diagram
-
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
gamma-linolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
show the reaction diagram
-
-
-
?
linoleoyl-[glycerolipid] + ferrocytochrome b5 + O2 + H+
gamma-linolenoyl-[glycerolipid] + ferricytochrome b5 + H2O
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome b5
-
-
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
DES6_BOROF
448
5
51635
Swiss-Prot
other Location (Reliability: 1)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Arabidopsis thaliana
expressed in Solanum lycopersicum
expressed in HEK-293 cells
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Chen, Q.; Nimal, J.; Li, W.; Liu, X.; Cao, W.
Delta-6 desaturase from borage converts linoleic acid to gamma-linolenic acid in HEK293 cells
Biochem. Biophys. Res. Commun.
410
484-488
2011
Borago officinalis
Manually annotated by BRENDA team
Cook, D.; Grierson, D.; Jones, C.; Wallace, A.; West, G.; Tucker, G.
Modification of fatty acid composition in tomato (Lycopersicon esculentum) by expression of a borage DELTA6-desaturase
Mol. Biotechnol.
21
123-128
2002
Borago officinalis (O04353)
Manually annotated by BRENDA team
Sayanova, O.; Shewry, P.R.; Napier, J.A.
Histidine-41 of the cytochrome b5 domain of the borage delta6 fatty acid desaturase is essential for enzyme activity
Plant Physiol.
121
641-646
1999
Borago officinalis (O04353)
Manually annotated by BRENDA team