Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 1.14.19.41 - sterol 22-desaturase and Organism(s) Solanum lycopersicum and UniProt Accession Q1T7C2

for references in articles please use BRENDA:EC1.14.19.41
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
A heme-thiolate protein (P-450). The enzyme, found in yeast and plants, catalyses the introduction of a double bond between the C-22 and C-23 carbons of certain sterols. In yeast the enzyme acts on ergosta-5,7,24(28)-trien-3beta-ol, a step in the biosynthesis of ergosterol. The enzyme from the plant Arabidopsis thaliana acts on sitosterol and 24-epi-campesterol, producing stigmasterol and brassicasterol, respectively.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Solanum lycopersicum
UNIPROT: Q1T7C2
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
The taxonomic range for the selected organisms is: Solanum lycopersicum
The enzyme appears in selected viruses and cellular organisms
Synonyms
cyp61, sterol c-22 desaturase, cyp710a, cyp710a1, cyp710a11, cyp710a2, c-22 sterol desaturase, erg5p, sterol c22-desaturase, cyp710a14, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
sterol C-22 desaturase
-
CYP710A
-
-
-
-
ERG5
-
-
-
-
sterol C-22 desaturase
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
ergosta-5,7,24(28)-trien-3beta-ol,NADPH:oxygen oxidoreductase (22,23-dehydrogenating)
A heme-thiolate protein (P-450). The enzyme, found in yeast and plants, catalyses the introduction of a double bond between the C-22 and C-23 carbons of certain sterols. In yeast the enzyme acts on ergosta-5,7,24(28)-trien-3beta-ol, a step in the biosynthesis of ergosterol. The enzyme from the plant Arabidopsis thaliana acts on sitosterol and 24-epi-campesterol, producing stigmasterol and brassicasterol, respectively.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
beta-sitosterol + NADPH + H+ + O2
stigmasterol + NADP+ + H2O
show the reaction diagram
-
-
-
?
beta-sitosterol + NADPH + H+ + O2
stigmasta-5,22-dien-3beta-ol + NADP+ + H2O
show the reaction diagram
-
-
-
-
?
additional information
?
-
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0037
beta-sitosterol
at pH 7.3 and 30°C
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.17
beta-sitosterol
at pH 7.3 and 30°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
46.7
beta-sitosterol
at pH 7.3 and 30°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
C7111_SOLLC
501
1
57519
Swiss-Prot
Secretory Pathway (Reliability: 2)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55000
x * 55000, SDS-PAGE
57511
x * 57511, calculated from amino acid sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in a baculovirus/Sf9 insect cell system
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the enzyme transcript increases 4fold in pericarp of ripening wild type fruit
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Whitaker, B.D.; Gapper, N.E.
Ripening-specific stigmasterol increase in tomato fruit is associated with increased sterol C-22 desaturase (CYP710A11) gene expression
J. Agric. Food Chem.
56
3828-3835
2008
Solanum lycopersicum
Manually annotated by BRENDA team
Morikawa, T.; Mizutani, M.; Aoki, N.; Watanabe, B.; Saga, H.; Saito, S.; Oikawa, A.; Suzuki, H.; Sakurai, N.; Shibata, D.; Wadano, A.; Sakata, K.; Ohta, D.
Cytochrome P450 CYP710A encodes the sterol C-22 desaturase in Arabidopsis and tomato
Plant Cell
18
1008-1022
2006
Arabidopsis thaliana, Solanum lycopersicum (Q1T7C2), Solanum lycopersicum
Manually annotated by BRENDA team