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Information on EC 1.14.19.29 - sphingolipid 8-(E/Z)-desaturase
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1.14.19.29 sphingolipid 8-(E/Z)-desaturaseIUBMB Comments
The enzymes from higher plants convert sphinganine, 4E-sphing-4-enine and phytosphinganine into E/Z-mixtures of Delta8-desaturated products displaying different proportions of geometrical isomers depending on plant species. The nature of the actual desaturase substrate has not yet been studied experimentally. The enzymes contain an N-terminal cytochrome b5 domain that acts as the direct electron donor to the active site of the desaturase . The homologous enzymes from some yeasts and diatoms, EC 1.14.19.18, sphingolipid 8-(E)-desaturase, act on sphing-4-enine ceramides and produce only the trans isomer.
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
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Synonyms
8 fatty acid desaturase, 8-sphingolipid desaturase, D8A, D8B, D8C, D8D, DELTA8 Des, Delta8 sphingolipid desaturase, DELTA8(E,Z)-sphingolipid desaturase, Delta8-fatty-acid desaturase 1, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
8 fatty acid desaturase
8-sphingolipid desaturase
D8A
DELTA8-sphingolipid desaturase
Delta8-sphingolipid desaturase 1
Delta8-sphingolipid desaturase 2
Delta8-sphingolipid desaturase 3
8 fatty acid desaturase
-
-
ambiguous
-
8-sphingolipid desaturase
-
-
ambiguous
-
DELTA8-sphingolipid desaturase
-
-
ambiguous
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+ = a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+ = a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+ = a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
(1)
-
-
-
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+ = a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
(1)
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+ = a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
(2)
-
-
-
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+ = a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
(2)
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
(4R)-4-hydroxysphinganine ceramide,ferrocytochrome b5:oxygen oxidoreductase (8,9 cis/trans-dehydrogenating)
The enzymes from higher plants convert sphinganine, 4E-sphing-4-enine and phytosphinganine into E/Z-mixtures of Delta8-desaturated products displaying different proportions of geometrical isomers depending on plant species. The nature of the actual desaturase substrate has not yet been studied experimentally. The enzymes contain an N-terminal cytochrome b5 domain that acts as the direct electron donor to the active site of the desaturase [1]. The homologous enzymes from some yeasts and diatoms, EC 1.14.19.18, sphingolipid 8-(E)-desaturase, act on sphing-4-enine ceramides and produce only the trans isomer.
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
(E)-sphing-4-enine + ferrocytochrome b5 + O2 + H+
(4E,8E)-sphinga-4,8-dienine + ferricytochrome b5 + H2O
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
C18-8-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
?
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
C20-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C-20-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
?
C20-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C20-phytosphingenine + ferricytochrome b5 + H2O
fluorinated phytosphinganine + ferrocytochrome b5 + O2 + H+
?
-
the presence of a fluorine atom at the position of the initial hydrogen removal C8-HR leads to a complete suppression of the desaturation reaction, while replacement of C8-HS with fluorine generates a mixture of mainly (Z)- and trace amounts of (E)-fluoroolefine
-
-
?
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+
(4R,8E)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+
(4R,8Z)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
sphinganine + ferrocytochrome b5 + O2 + H+
8-sphingenine + ferricytochrome b5 + H2O
-
-
-
?
sphinganine ceramide + ferrocytochrome b5 + O2 + H+
8-sphingenine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
the ratio of trans:cis products is approximately 4:1 for isoform SLD1 and 7:1 for isoform SLD2
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
7:1 product mixture of (4R,8E)- and (4R,8Z)-4-hydroxy-sphing-8-enine ceramide
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
39.8% conversion rate
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
32.5% conversion rate
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
7.5% conversion rate
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
32.5% conversion rate
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
the enzyme exhibits preference for (Z)-isomer formation
-
?
(E)-sphing-4-enine + ferrocytochrome b5 + O2 + H+
(4E,8E)-sphinga-4,8-dienine + ferricytochrome b5 + H2O
-
-
-
-
?
(E)-sphing-4-enine + ferrocytochrome b5 + O2 + H+
(4E,8E)-sphinga-4,8-dienine + ferricytochrome b5 + H2O
-
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
-
?
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
-
?
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
?
C20-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C20-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
-
?
C20-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C20-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
-
?
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+
(4R,8E)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+
(4R,8E)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
the enzyme expressed in Saccharomyces cerevisiae converts 4-hydroxy-sphinganine (phytosphinganine) into a 7:1 mixture of (4R,8E)- and (4R,8Z)-4-hydroxy-8-sphingenine. Both desaturation steps leading to either the 8E or the 8Z isomer of 4-hydroxy-8-sphingenine involved the syn elimination of two vicinal hydrogen atoms
-
-
?
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+
(4R,8Z)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
-
(2)
-
?
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+
(4R,8Z)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
the enzyme expressed in Saccharomyces cerevisiae converts 4-hydroxy-sphinganine (phytosphinganine) into a 7:1 mixture of (4R,8E)- and (4R,8Z)-4-hydroxy-8-sphingenine. Both desaturation steps leading to either the 8E or the 8Z isomer of 4-hydroxy-8-sphingenine involved the syn elimination of two vicinal hydrogen atoms
(2)
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
no activity with palmitoleic acid, linoleic acid, and alpha-linolenic acid
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the enzyme lacks DELTA6-desaturase activity
-
-
?
additional information
?
-
-
the enzyme lacks DELTA6-desaturase activity
-
-
?
additional information
?
-
-
the DELTA8-sphingolipid desaturase is able to catalyze desaturation at the C8 position of the long-chain base of sphingolipids in plants. The recombinant enzyme catalyzes the C18-phytosphingenine desaturation to produce 8(Z)- and 8(E)-C18-phytosphingenine
-
-
?
additional information
?
-
-
isozyme PtD8A preferably forms (8E)-products, ratio 8(Z):8(E) is 0.25
-
-
?
additional information
?
-
-
isozyme PtD8B preferably forms (8E)-products, ratio 8(Z):8(E) is 0.17
-
-
?
additional information
?
-
-
isozyme PtD8C preferably forms (8Z)-products, ratio 8(Z):8(E) is 12.71
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
additional information
?
-
the recombinant enzyme is a functional DELTA8-sphingolipid desaturase, which catalyzes different ratios of two products, namely, (8Z)- and (8E)-C18-phytosphingenine
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
(E)-sphing-4-enine + ferrocytochrome b5 + O2 + H+
(4E,8E)-sphinga-4,8-dienine + ferricytochrome b5 + H2O
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
C18-8-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
?
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
C20-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C-20-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
?
C20-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C20-phytosphingenine + ferricytochrome b5 + H2O
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+
(4R,8E)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
phytosphinganine + 2 ferrocytochrome b5 + O2 + 2 H+
(4R,8Z)-4-hydroxy-8-sphingenine + 2 ferricytochrome b5 + 2 H2O
-
(2)
-
?
sphinganine + ferrocytochrome b5 + O2 + H+
8-sphingenine + ferricytochrome b5 + H2O
-
-
-
?
sphinganine ceramide + ferrocytochrome b5 + O2 + H+
8-sphingenine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
additional information
?
-
-
the DELTA8-sphingolipid desaturase is able to catalyze desaturation at the C8 position of the long-chain base of sphingolipids in plants. The recombinant enzyme catalyzes the C18-phytosphingenine desaturation to produce 8(Z)- and 8(E)-C18-phytosphingenine
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
the ratio of trans:cis products is approximately 4:1 for isoform SLD1 and 7:1 for isoform SLD2
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + (4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
7:1 product mixture of (4R,8E)- and (4R,8Z)-4-hydroxy-sphing-8-enine ceramide
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
39.8% conversion rate
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
32.5% conversion rate
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8E)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
7.5% conversion rate
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
-
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
32.5% conversion rate
-
-
?
(4R)-4-hydroxysphinganine ceramide + ferrocytochrome b5 + O2 + H+
(4R,8Z)-4-hydroxysphing-8-enine ceramide + ferricytochrome b5 + H2O
-
the enzyme exhibits preference for (Z)-isomer formation
-
?
(E)-sphing-4-enine + ferrocytochrome b5 + O2 + H+
(4E,8E)-sphinga-4,8-dienine + ferricytochrome b5 + H2O
-
-
-
-
?
(E)-sphing-4-enine + ferrocytochrome b5 + O2 + H+
(4E,8E)-sphinga-4,8-dienine + ferricytochrome b5 + H2O
-
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8E)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
a (4R)-4-hydroxysphinganine ceramide + 2 ferrocytochrome b5 + O2 + 2 H+
a (4R,8Z)-4-hydroxysphing-8-enine ceramide + 2 ferricytochrome b5 + 2 H2O
-
-
-
?
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
-
?
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
-
?
C18-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C18-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
?
C20-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C20-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
-
?
C20-phytosphinganine + ferrocytochrome b5 + O2 + H+
DELTA8-trans/cis-C20-phytosphingenine + ferricytochrome b5 + H2O
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
cytochrome b5