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Information on EC 1.14.19.2 - stearoyl-[acyl-carrier-protein] 9-desaturase and Organism(s) Arabidopsis thaliana and UniProt Accession Q84VY3

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IUBMB Comments
The enzyme is found in the lumen of plastids, where de novo biosynthesis of fatty acids occurs, and acts on freshly synthesized saturated fatty acids that are still linked to acyl-carrier protein. The enzyme determines the position of the double bond by its distance from the carboxylic acid end of the fatty acid. It also acts on palmitoyl-[acyl-carrier-protein] [4,5].
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: Q84VY3
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The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Reaction Schemes
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stearoyl-[acyl-carrier protein]
+
2
reduced ferredoxin [iron-sulfur] cluster
+
O2
+
2
H+
=
oleoyl-[acyl-carrier protein]
+
2
oxidized ferredoxin [iron-sulfur] cluster
+
2
H2O
+
2
reduced ferredoxin [iron-sulfur] cluster
+
+
2
=
+
2
oxidized ferredoxin [iron-sulfur] cluster
+
2
Synonyms
stearoyl-acp desaturase, stearoyl-acyl carrier protein desaturase, sacpd-c, desa1, desa2, zmsad1, sacpd, stearoyl-acyl carrier protein (acp) desaturase, nbsacpd-c, gmsacpd-c, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
desaturase, acyl-[acyl carrier protein]
-
-
-
-
SSI2 desaturase
-
stearoyl-ACP desaturase
-
-
-
-
stearoyl-acyl carrier protein desaturase
-
stearoyl-acyl carrier protein desaturase 3
-
stearoyl-acyl carrier protein desaturase-1
-
stearoyl-[acyl carrier protein] desaturase
-
-
-
-
stearyl acyl carrier protein desaturase
-
-
-
-
stearyl-ACP desaturase
-
-
-
-
stearyl-acyl carrier protein desaturase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
reduction
SYSTEMATIC NAME
IUBMB Comments
stearoyl-[acyl-carrier protein],reduced ferredoxin:oxygen oxidoreductase (9,10 cis-dehydrogenating)
The enzyme is found in the lumen of plastids, where de novo biosynthesis of fatty acids occurs, and acts on freshly synthesized saturated fatty acids that are still linked to acyl-carrier protein. The enzyme determines the position of the double bond by its distance from the carboxylic acid end of the fatty acid. It also acts on palmitoyl-[acyl-carrier-protein] [4,5].
CAS REGISTRY NUMBER
COMMENTARY hide
37256-86-3
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
palmitoyl-[acyl-carrier protein] + AH2 + O2
palmitoleoyl-[acyl-carrier protein] + A + H2O
show the reaction diagram
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + electron acceptor + H2O
show the reaction diagram
stearoyl-[acyl-carrier protein] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
oleoyl-[acyl-carrier protein] + oxidized ferredoxin [iron-sulfur] cluster + H2O
show the reaction diagram
-
-
-
?
stearoyl-[acyl-carrier-protein] + reduced acceptor + O2
oleoyl-[acyl-carrier-protein] + acceptor + H2O
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
stearoyl-[acyl-carrier protein] + electron donor + O2
oleoyl-[acyl-carrier protein] + electron acceptor + H2O
show the reaction diagram
stearoyl-[acyl-carrier protein] + reduced ferredoxin [iron-sulfur] cluster + O2 + H+
oleoyl-[acyl-carrier protein] + oxidized ferredoxin [iron-sulfur] cluster + H2O
show the reaction diagram
-
-
-
?
stearoyl-[acyl-carrier-protein] + reduced acceptor + O2
oleoyl-[acyl-carrier-protein] + acceptor + H2O
show the reaction diagram
-
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
seven isoforms
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
RT-PCR, gene-specific primers for the isoform
Manually annotated by BRENDA team
RT-PCR, gene-specific primers for the isoform
Manually annotated by BRENDA team
RT-PCR, gene-specific primers for the isoform
Manually annotated by BRENDA team
RT-PCR, gene-specific primers for the isoform
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
seed-specific overexpression of the exogenous ZmSAD1 gene in Arabidopsis thaliana significantly reduces the content of stearic acid and the ratio of saturated to unsaturated fatty acids
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
STAD6_ARATH
391
0
44156
Swiss-Prot
Mitochondrion (Reliability: 5)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
44180
calculated from amino acid sequence
45020
calculated from amino acid sequence
45290
calculated from amino acid sequence
45480
calculated from amino acid sequence
45690
calculated from amino acid sequence
45860
calculated from amino acid sequence
46000
x * 46000, SDS-PAGE
46950
calculated from amino acid sequence
50500
determined by SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 46000, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by Ni-NTA purification system
overexpressed S-ACP-DES1 enzyme
overexpressed S-ACP-DES3 enzyme
overexpressed S-ACP-DES4 enzyme
overexpressed S-ACP-DES5 enzyme
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21 Star (DE3) cells
gene SAD1, phylogenetic analysis
into the pUCm-T vector and subcloned into the vector pET30a for expression in Escherichia coli BL21DE3 cells
S-ACP-DES1 expressed in Escherichia coli
S-ACP-DES3 enzyme expressed in Escherichia coli
S-ACP-DES4 enzyme expressed in Escherichia coli
S-ACP-DES5 enzyme expressed in Escherichia coli
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
seed-specific down-regulation of endogenous AtSAD genes in Arabidopsis significantly increases the content of stearic and long-chain saturated fatty acids and the ratio of saturated to unsaturated fatty acids, whereas it significantly decreases the oleic acid content. Composition of fatty acids in the Arabidopsis seeds harbouring the exogenous ZmSAD1, anti-ZmSAD1, or ZmSAD1 RNAi constructs, overview
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
biofuel production
biodiesel production
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kachroo, A.; Shanklin, J.; Whittle, E.; Lapchyk, L.; Hildebrand, D.; Kachroo, P.
The Arabidopsis stearoyl-acyl carrier protein-desaturase family and the contribution of leaf isoforms to oleic acid synthesis
Plant Mol. Biol.
63
257-271
2007
Arabidopsis thaliana (O22832), Arabidopsis thaliana (Q84VY3), Arabidopsis thaliana (Q9LF04), Arabidopsis thaliana (Q9LF05), Arabidopsis thaliana (Q9M879), Arabidopsis thaliana (Q9M880), Arabidopsis thaliana (Q9M881)
Manually annotated by BRENDA team
Cao, Y.; Xian, M.; Yang, J.; Xu, X.; Liu, W.; Li, L.
Heterologous expression of stearoyl-acyl carrier protein desaturase (S-ACP-DES) from Arabidopsis thaliana in Escherichia coli
Protein Expr. Purif.
69
209-214
2010
Arabidopsis thaliana (O22832), Arabidopsis thaliana
Manually annotated by BRENDA team
Scaglia, B.; Cassani, E.; Pilu, R.; Adani, F.
Expression of Arabidopsis thaliana S-ACP-DES3 in Escherichia coli for high-performance biodiesel production
RSC Adv.
4
63387-63392
2014
Arabidopsis thaliana (Q9LF05)
-
Manually annotated by BRENDA team
Du, H.; Huang, M.; Hu, J.; Li, J.
Modification of the fatty acid composition in Arabidopsis and maize seeds using a stearoyl-acyl carrier protein desaturase-1 (ZmSAD1) gene
BMC Plant Biol.
16
137
2016
Arabidopsis thaliana (Q9LF04), Zea mays (B4F9Q1), Zea mays
Manually annotated by BRENDA team