Information on EC 1.14.19.10 - icosanoyl-CoA 5-desaturase

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The expected taxonomic range for this enzyme is: Limnanthes alba subsp. alba

EC NUMBER
COMMENTARY hide
1.14.19.10
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RECOMMENDED NAME
GeneOntology No.
icosanoyl-CoA 5-desaturase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
icosanoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+ = (Z)-icos-5-enoyl-CoA + 2 ferricytochrome b5 + 2 H2O
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
(5Z)-icosenoate biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
icosanoyl-CoA,ferrocytochrome b5:oxygen oxidoreductase (5,6 cis-dehydrogenating)
The enzyme, characterized from the plant Limnanthes douglasii (meadowfoam), is involved in the biosynthesis of (5Z)-icos-5-enoate, an unusual monounsaturated fatty acid that makes up to 60% of the total fatty acids in Limnanthes sp. seed oil. The enzyme only acts on saturated fatty acids.
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
cv. MF164, var. Ross
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Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
evolution
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the enzyme is a member of the large DELTAa9-FADS-like or ADS subfamily found in eukaryotes including vertebrates, insects, higher plants and fungi, as well as a wide range of bacteria. These non-heme, iron-containing, endoplasmic reticulum membrane-bound enzymes exhibit a diversity of subcellular localization, lipid substrate utilization, regiospecificity, and biological function
metabolism
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mapping genes in meadowfoam glucosinolate metabolism, overview
additional information
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meadowfoam seed oil is the richest known source of DELTA5 monounsaturated fatty acids and presently the sole commercial source of DELTA5-unsaturated very long chain fatty acids, a high 20:1DELTA5 phenotype of meadowfoam oil
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
icosanoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+
(Z)-icos-5-enoyl-CoA + 2 ferricytochrome b5 + 2 H2O
show the reaction diagram
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?
additional information
?
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very long chain fatty acids are desaturated at the DELTA5 position
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
icosanoyl-CoA + 2 ferrocytochrome b5 + O2 + 2 H+
(Z)-icos-5-enoyl-CoA + 2 ferricytochrome b5 + 2 H2O
show the reaction diagram
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-
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?
additional information
?
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very long chain fatty acids are desaturated at the DELTA5 position
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome b5
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SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
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the enzyme is highly expressed in mid-development embryos
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
additional information
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the enzyme lacks a chloroplastic transit peptide
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Manually annotated by BRENDA team
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene DELTA5DS, gene mapping and genotyping, Construction and annotation of a developing embryo cDNA library, gene DELTA5DS DNA and amino acid sequence determination and analysis, sequence comparisons, RT-PCR enzyme expression analysis
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