Any feedback?
Information on EC 1.14.18.3 - methane monooxygenase (particulate)
for references in articles please use BRENDA:EC1.14.18.3Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
1.14.18.3 methane monooxygenase (particulate)IUBMB Comments
Contains copper. It is membrane-bound, in contrast to the soluble methane monooxygenase (EC 1.14.13.25).
Specify your search results
Word Map
- 1.14.18.3
-
pmmos
- methanotrophs
- methylococcus
- capsulatus
- bath
- methylocystis
- methylosinus
- ch4
- trichosporium
-
pmocab
- methylomicrobium
- duroquinol
- environmental protection
- analysis
-
trinuclear
-
nadh:quinone
-
monocopper
-
diiron
-
ammonia-oxidizing
- energy production
- degradation
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
particulate mmo, spmob, membrane-bound methane monooxygenase, membrane-associated methane monooxygenase, particulate methane mono-oxygenase, pmmo2, pmmo hydroxylase, particulate methane monooxygenase a, pmmo1, copper-containing membrane monooxygenase, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
copper-containing membrane monooxygenase
copper-containing membrane-bound monooxygenase
CuMMO
membrane-associated methane monooxygenase
membrane-bound methane monooxygenase
membrane-embedded methane monooxygenase
methane hydroxylase
MMO
particulate methane mono-oxygenase
particulate methane monooxygenase
particulate methane monooxygenase A
D0FK34, D0FK37, D0FK39, D0FK43, D0FK44, D0FK45, D0FK53, D0FK57, D0FK58, D0FK59, D0FK61, D0FK62, D0FK63, D0FK65, D0FK67, D0FKJ4, D0FKK3, D0FKS0, D0FL26, D0FL48
-
particulate methane-oxidizing complex
particulate MMO
PMH
pMMO
pMMO hydroxylase
pMMO-H
pMMO1
pMMO2
PmoA
sMMO
soluble methane monooxygenase
spmoB
-
recombinant soluble domains of the pmoB subunit of particulate methane monooxygenase
copper-containing membrane-bound monooxygenase
-
copper-containing membrane-bound monooxygenase
-
particulate methane monooxygenase
-
-
particulate methane monooxygenase
-
particulate methane monooxygenase
-
-
particulate methane monooxygenase
-
-
pMMO
-
-
pMMO
-
consists of two protein components: NADH oxidoreductase (pMMOR) and hydroxylase (pMMOH)
pMMO
-
pMMO
-
consists of two protein components: NADH oxidoreductase (pMMOR) and hydroxylase (pMMOH)
-
pMMO
-
-
PmoA
D0FK34, D0FK37, D0FK39, D0FK43, D0FK44, D0FK45, D0FK53, D0FK57, D0FK58, D0FK59, D0FK61, D0FK62, D0FK63, D0FK65, D0FK67, D0FKJ4, D0FKK3, D0FKS0, D0FL26, D0FL48
-
sMMO
-
consists of three components: hydroxylase (sMMOH), NADH-dependent reductase (sMMOR), and the regulatory protein B (sMMOB)
sMMO
-
consists of three components: hydroxylase (sMMOH), NADH-dependent reductase (sMMOR), and the regulatory protein B (sMMOB)
-
soluble methane monooxygenase
Q25BV9; Q25BW0; Q25BW2; Q25BW3
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
methane + quinol + O2 = methanol + quinone + H2O
-
-
-
-
methane + quinol + O2 = methanol + quinone + H2O
mechanism of pMMO
-
methane + quinol + O2 = methanol + quinone + H2O
putative mechanism for the reaction of the mononuclear site with methane, structure modeling, overview
methane + quinol + O2 = methanol + quinone + H2O
reaction mechanism of enzyme pMMO, via O2 activation intermediates, detailed overview
methane + quinol + O2 = methanol + quinone + H2O
reaction mechanism of enzyme pMMO, via O2 activation intermediates, detailed overview
-
methane + quinol + O2 = methanol + quinone + H2O
mechanism of pMMO
-
-
methane + quinol + O2 = methanol + quinone + H2O
putative mechanism for the reaction of the mononuclear site with methane, structure modeling, overview
-
-
methane + quinol + O2 = methanol + quinone + H2O
reaction mechanism of enzyme pMMO, via O2 activation intermediates, detailed overview
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
methane,quinol:oxygen oxidoreductase
Contains copper. It is membrane-bound, in contrast to the soluble methane monooxygenase (EC 1.14.13.25).
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
1,1,1-trifluoropropane + reduced acceptor + H+ + O2
(2R)-1,1,1-trifluoropropan-2-ol + (2S)-1,1,1-trifluoropropan-2-ol + acceptor + H2O
-
-
the S stereoisomer is the dominant product
-
?
1,3-butadiene + duroquinol + O2
1,2-epoxybut-3-ene + duroquinone + H2O
-
-
100% 1,2-epoxybut-3-ene is produced, 36% (R)-selectivity, 64% (S)-selectivity
-
?
1-bromopropane + duroquinol + O2
1-bromo-2-propanol + 1-propanol + 1-bromo-3-propanol + duroquinone + H2O
-
-
72% 1-bromo-2-propanol (70% (R)-selectivity, 30% (S)-selectivity), 24% 1-propanol and 4% 1-bromo-3-propanol are produced
-
?
1-bromopropene + duroquinol + O2
1-bromo-2,3-epoxypropane + allyl-alcohol + 1-propanol + duroquinone + H2O
-
-
63% 1-bromo-2,3-epoxypropane, 31% allyl-alcohol and 6% 1-propanol are produced
-
?
1-butene + duroquinol + O2
1,2-epoxybutane + duroquinone + H2O
-
-
100% epoxybutane is produced, 36% (R)-selectivity, 64% (S)-selectivity
-
?
1-butene + reduced acceptor + H+ + O2
1,2-epoxybutane + acceptor + H2O
-
-
-
-
?
1-chloropropane + duroquinol + O2
1-chloro-2-propanol + 1-propanol + 1-chloro-3-propanol + duroquinone + H2O
-
-
64% 1-chloro-2-propanol (70% (R)-selectivity, 30% (S)-selectivity), 29% 1-propanol and 7% 1-chloro-3-propanol are produced
-
?
1-chloropropene + duroquinol + O2
1-chloro-2,3-epoxypropane + allyl-alcohol + 1-propanol + duroquinone + H2O
-
-
67% 1-chloro-2,3-epoxypropane, 23% allyl-alcohol and 10% 1-propanol are produced
-
?
2 1-butene + 2 reduced acceptor + 2 H+ + 2 O2
3-buten-2-ol + 1,2-epoxybutane + 2 acceptor + 2 H2O
-
-
-
-
?
2 butane + 2 reduced acceptor + 2 H+ + 2 O2
1-butanol + 2-butanol + 2 acceptor + 2 H2O
-
-
-
-
?
2 pentane + 2 reduced acceptor + 2 H+ + 2 O2
1-pentanol + 2-pentanol + 2 acceptor + 2 H2O
-
-
-
-
?
2 propane + 2 reduced acceptor + 2 H+ + 2 O2
1-propanol + 2-propanol + 2 acceptor + 2 H2O
-
-
-
-
?
2-bromopropane + duroquinol + O2
2-bromo-1-propanol + acetone + duroquinone + H2O
-
-
2-bromo-1-propanol shows 26% (R)-selectivity and 74% (S)-selectivity
-
?
2-chloropropane + duroquinol + O2
2-chloro-1-propanol + ? + duroquinone + H2O
-
-
1-chloro-2-propanol shows 26% (R)-selectivity and 74% (S)-selectivity
-
?
3 cis-2-butene + reduced acceptor + H+ + O2
meso-2,3-dimethyloxirane + acceptor + H2O
-
-
-
-
?
3,3,3-trifluoroprop-1-ene + reduced acceptor + H+ + O2
(2S)-2-(trifluoromethyl)oxirane + (2R)-2-(trifluoromethyl)oxirane + acceptor + H2O
-
-
the S stereoisomer is the dominant product
-
?
3,3,3-trifluoropropene + reduced acceptor + H+ + O2
3,3,3-trifluoro-1,2-epoxypropane + acceptor + H2O
-
-
-
-
?
butane + duroquinol + O2
2-butanol + butanal + duroquinone + H2O
-
-
91% 2-butanal and 9% butanal are produced
-
?
cis-2-butene + duroquinol + O2
cis-2,3-epoxybutane + duroquinone + H2O
-
-
cis-2,3-epoxybutane is produced
-
?
cis-but-2-ene + reduced acceptor + H+ + O2
cis-2,3-dimethyloxirane + acceptor + H2O
-
-
-
-
?
ethane + duroquinol + O2
ethanal + duroquinone + H2O
-
-
100% ethanal is produced
-
?
ethylene + duroquinol + O2
epoxyethane + duroquinone + H2O
-
-
100% epoxyethane produced
-
?
methane + trans-dichloroethylene + vinyl chloride + trichloroethylene + O2
?
-
-
-
-
?
pentane + duroquinol + O2
2-pentanol + pentanal + duroquinone + H2O
-
-
31% 2-pentanal and 69% pentanal are produced
-
?
propane + duroquinol + O2
2-propanol + propanal + duroquinone + H2O
-
-
84% 2-propanal and 16% propanal are produced
-
?
propene + duroquinol + O2
1,2-epoxypropane + duroquinone + H2O
-
-
57% (R)-selectivity, 43% (S)-selectivity
-
?
propylene + decylubiquinol + O2
propylene oxide + decylubiquinone + H2O
-
-
-
-
?
propylene + duroquinol + O2
epoxypropane + allyl-alcohol + 1-propanol + duroquinone + H2O
-
-
95% epoxypropane, 4.6% allyl-alcohol and 0.4% butanal are produced
-
?
propylene + menaquinol + O2
propylene oxide + menaquinone + H2O
-
-
-
-
?
propylene + trimethylquinol + O2
propylene oxide + trimethylquinone + H2O
-
-
-
-
?
trans-2-butene + duroquinol + O2
trans-2,3-epoxybutane + trans-2-butane-1-al + duroquinone + H2O
-
-
41% trans-2,3-epoxybutane and 59% trans-2-butane-1-al are produced
-
?
trans-2-butene + reduced acceptor + H+ + O2
2,3-dimethyloxirane + acceptor + H2O
-
-
-
-
?
trans-but-2-ene + reduced acceptor + H+ + O2
(2R,3R)-trans-2,3-dimethyloxirane + (2S,3S)-trans-2,3-dimethyloxirane + acceptor + H2O
-
-
the S,S stereoisomer is the dominant product
-
?
methane + duroquinol + O2
methanol + duroquinone + H2O
-
-
-
-
?
methane + duroquinol + O2
methanol + duroquinone + H2O
-
-
-
?
methane + duroquinol + O2
methanol + duroquinone + H2O
-
-
-
-
?
methane + duroquinol + O2
methanol + duroquinone + H2O
-
-
-
?
methane + duroquinol + O2
methanol + duroquinone + H2O
-
-
100% methanol is produced
-
?
methane + NADH + O2
methanol + NAD+ + H2O
-
-
-
?
methane + quinol + O2
methanol + quinone + H2O
-
-
-
?
methane + quinol + O2
methanol + quinone + H2O
-
-
-
?
methane + quinol + O2
methanol + quinone + H2O
-
-
-
?
methane + quinol + O2
methanol + quinone + H2O
Methylococcus capsulatus (Bath) is a methanotroph that possesses both a membrane-embedded (pMMO) and a soluble methane monooxygenase (sMMO). Major changes takes place in the respiratory chain between pMMO- and sMMO-producing cells. Quinones are predominantly used as the electron donors for methane oxidation by pMMO. During production of particulate methane monooxygenase, the majority of quinones are directed to methane oxidation
-
-
?
methane + quinol + O2
methanol + quinone + H2O
enzyme pMMO activity is dependent on oxygen concentrations
-
-
?
methane + quinol + O2
methanol + quinone + H2O
Methylococcus capsulatus (Bath) is a methanotroph that possesses both a membrane-embedded (pMMO) and a soluble methane monooxygenase (sMMO). Major changes takes place in the respiratory chain between pMMO- and sMMO-producing cells. Quinones are predominantly used as the electron donors for methane oxidation by pMMO. During production of particulate methane monooxygenase, the majority of quinones are directed to methane oxidation
-
-
?
methane + quinol + O2
methanol + quinone + H2O
-
-
-
?
methane + quinol + O2
methanol + quinone + H2O
enzyme pMMO activity is dependent on oxygen concentrations
-
-
?
methane + quinol + O2
methanol + quinone + H2O
-
-
-
?
methane + quinol + O2
methanol + quinone + H2O
Methylococcus capsulatus Bath.
-
Methylococcus capsulatus (Bath) is a methanotroph that possesses both a membrane-embedded (pMMO) and a soluble methane monooxygenase (sMMO). Major changes takes place in the respiratory chain between pMMO- and sMMO-producing cells. Quinones are predominantly used as the electron donors for methane oxidation by pMMO. During production of particulate methane monooxygenase, the majority of quinones are directed to methane oxidation
-
-
?
methane + quinol + O2
methanol + quinone + H2O
-
methane activation occurs at the Cu centers of particulate methane monooxygenase
-
-
?
methane + quinol + O2
methanol + quinone + H2O
Methylocystis sp. Rockwell
-
methane activation occurs at the Cu centers of particulate methane monooxygenase
-
-
?
methane + quinol + O2
methanol + quinone + H2O
-
methane activation occurs at the Cu centers of particulate methane monooxygenase
-
-
?
methane + reduced acceptor + H* + O2
methanol + acceptor + H2O
-
-
-
-
?
methane + reduced acceptor + H* + O2
methanol + acceptor + H2O
-
-
-
?
methane + reduced acceptor + H* + O2
methanol + acceptor + H2O
-
-
671965, 672301, 674005, 675831, 684112, 684114, 685113, 686085, 687284, 701759, 702501, 703343, 706752
-
-
?
methane + reduced acceptor + H* + O2
methanol + acceptor + H2O
-
-
-
?
methane + reduced acceptor + H* + O2
methanol + acceptor + H2O
-
-
-
?
methane + reduced acceptor + H* + O2
methanol + acceptor + H2O
-
-
-
?
methane + reduced acceptor + H* + O2
methanol + acceptor + H2O
-
-
-
-
?
methane + reduced acceptor + H* + O2
methanol + acceptor + H2O
-
-
-
?
methane + reduced acceptor + H* + O2
methanol + acceptor + H2O
-
-
-
?
methane + reduced acceptor + H* + O2
methanol + acceptor + H2O
-
-
-
?
methane + reduced acceptor + H* + O2
methanol + acceptor + H2O
-
-
-
-
?
methane + reduced acceptor + H* + O2
methanol + acceptor + H2O
-
-
-
-
?
methane + reduced acceptor + H* + O2
methanol + acceptor + H2O
-
-
-
-
?
methane + reduced acceptor + H* + O2
methanol + acceptor + H2O
-
-
-
-
?
methane + reduced acceptor + H* + O2
methanol + acceptor + H2O
-
-
-
?
methane + reduced acceptor + H* + O2
methanol + acceptor + H2O
-
-
-
?
methane + reduced acceptor + H* + O2
methanol + acceptor + H2O
-
-
-
-
?
methane + reduced acceptor + H* + O2
methanol + acceptor + H2O
-
-
-
-
?
methane + reduced acceptor + H* + O2
methanol + acceptor + H2O
-
in the presence of pMMO substrate methane, the H2O2 formation is diminished, which is likely to be caused by the consumption of electrons by methane oxidation
-
-
?
methane + reduced acceptor + H* + O2
methanol + acceptor + H2O
Q25BV2; Q25BV3; Q25BV4, Q25BV9; Q25BW0; Q25BW2; Q25BW3
-
-
-
?
methane + reduced acceptor + H* + O2
methanol + acceptor + H2O
Q25BV2; Q25BV3; Q25BV4, Q25BV9; Q25BW0; Q25BW2; Q25BW3
-
-
-
?
methane + reduced acceptor + H* + O2
methanol + acceptor + H2O
Soil bacterium
-
pmoA cluster JR3 may be the most important methane oxidizer for arid soils
-
-
?
methane + reduced acceptor + H* + O2
methanol + acceptor + H2O
D0FK34, D0FK37, D0FK39, D0FK43, D0FK44, D0FK45, D0FK53, D0FK57, D0FK58, D0FK59, D0FK61, D0FK62, D0FK63, D0FK65, D0FK67, D0FKJ4, D0FKK3, D0FKS0, D0FL26, D0FL48
-
-
-
?
methane + succinate + O2
methanol + fumarate + H2O
membrane-bound enzyme only
-
-
?
methane + succinate + O2
methanol + fumarate + H2O
membrane-bound enzyme only
-
-
?
n-butane + reduced acceptor + O2
2-butanol + acceptor + H2O
-
-
-
?
n-butane + reduced acceptor + O2
2-butanol + acceptor + H2O
-
-
-
-
?
n-pentane + reduced acceptor + O2
2-pentanol + acceptor + H2O
-
-
-
?
n-pentane + reduced acceptor + O2
2-pentanol + acceptor + H2O
-
-
-
-
?
propene + reduced acceptor + H+ + O2
1,2-epoxypropane + acceptor + H2O
-
-
-
-
?
propene + reduced acceptor + H+ + O2
1,2-epoxypropane + acceptor + H2O
-
enzyme form sMMO
-
?
propene + reduced acceptor + H+ + O2
1,2-epoxypropane + acceptor + H2O
-
-
-
-
?
propene + reduced acceptor + H+ + O2
1,2-epoxypropane + acceptor + H2O
-
enzyme form sMMO
-
?
propylene + 2,3-dimethylquinol + O2
propylene oxide + 2,3-dimethylquinone + H2O
-
-
-
-
?
propylene + 2,3-dimethylquinol + O2
propylene oxide + 2,3-dimethylquinone + H2O
-
-
-
-
?
propylene + coenzyme Q0 + O2
propylene oxide + reduced coenzyme Q0 + H2O
-
-
-
-
?
propylene + coenzyme Q0 + O2
propylene oxide + reduced coenzyme Q0 + H2O
-
-
-
-
?
propylene + decyl-plastoquinol + O2
propylene oxide + decyl-plastoquinone + H2O
-
higher activity compared to duroquinol
-
-
?
propylene + decyl-plastoquinol + O2
propylene oxide + decyl-plastoquinone + H2O
-
higher activity compared to duroquinol
-
-
?
propylene + duroquinol + O2
propylene epoxide + duroquinone + H2O
-
-
-
-
?
propylene + duroquinol + O2
propylene epoxide + duroquinone + H2O
-
-
-
-
?
propylene + duroquinol + O2
propylene oxide + duroquinone + H2O
-
-
-
-
?
propylene + duroquinol + O2
propylene oxide + duroquinone + H2O
-
-
-
-
?
propylene + duroquinol + O2
propylene oxide + duroquinone + H2O
-
-
-
-
?
propylene + duroquinol + O2
propylene oxide + reduced duroquinol + H2O
-
-
-
-
?
propylene + duroquinol + O2
propylene oxide + reduced duroquinol + H2O
-
-
-
-
?
additional information
?
-
-
unlike the sMMO, the pMMO enzyme has relatively narrow substrate specificity, oxidising alkanes and alkenes of up to five carbons but not aromatic compounds
-
-
?
additional information
?
-
-
pMMO has narrower substrate specificity but higher activity with smaller hydrocarbons like methane, ethane, and propene compared to sMMO
-
-
?