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Information on EC 1.14.18.2 - CMP-N-acetylneuraminate monooxygenase

for references in articles please use BRENDA:EC1.14.18.2
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EC Tree
IUBMB Comments
This enzyme contains both a Rieske-type [2Fe-2S] cluster and a second iron site. The ferricytochrome b5 produced is reduced by NADH and cytochrome-b5 reductase (EC 1.6.2.2). The enzyme can be activated by Fe2+ or Fe3+.
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This record set is specific for:
UNIPROT: Q9Y471
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Word Map
  • 1.14.18.2
  • n-glycolylneuraminic
  • neu5gc
  • sialic
  • xenotransplantation
  • cmp-neu5gc
  • n-glycolyl
  • hanganutziu-deicher
  • non-gal
  • human-like
  • xenoantigenicity
  • anti-neu5gc
  • hominid
  • alpha-1,3-galactosyltransferase
  • asterias
  • neu5gc-containing
  • medicine
  • synthesis
  • analysis
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
cmp-neu5ac hydroxylase, cmp-neuac hydroxylase, cmp-n-acetylneuraminic acid hydroxylase, pcmah, cytidine monophosphate-n-acetylneuraminic acid hydroxylase, cytidine monophospho-n-acetylneuraminic acid hydroxylase, cmp-n-acetylneuraminate hydroxylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CMAH
C1K3L2
-
CMP-N-acetylneuraminic acid hydroxylase
C1K3L2
-
CMAH
-
-
-
-
CMP-N-acetylneuraminate hydroxylase
-
-
-
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CMP-N-acetylneuraminate,NAD(P)H:oxygen oxidoreductase (hydroxylating)
-
-
-
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CMP-N-acetylneuraminic acid hydroxylase
-
-
-
-
CMP-Neu5Ac hydroxylase
-
-
-
-
oxygenase, cytidine monophosphoacetylneuraminate mono-
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
C1K3L2
-
reduction
C1K3L2
-
redox reaction
-
-
-
-
oxidation
-
-
-
-
reduction
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
CMP-N-acetylneuraminate,ferrocytochrome-b5:oxygen oxidoreductase (N-acetyl-hydroxylating)
This enzyme contains both a Rieske-type [2Fe-2S] cluster and a second iron site. The ferricytochrome b5 produced is reduced by NADH and cytochrome-b5 reductase (EC 1.6.2.2). The enzyme can be activated by Fe2+ or Fe3+.
CAS REGISTRY NUMBER
COMMENTARY hide
116036-67-0
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
CMP-N-acetylneuraminate + ferrocytochrome b5 + O2 + H+
CMP-N-glycoloylneuraminate + ferricytochrome b5 + H2O
show the reaction diagram
C1K3L2
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
CMP-N-acetylneuraminate + ferrocytochrome b5 + O2 + H+
CMP-N-glycoloylneuraminate + ferricytochrome b5 + H2O
show the reaction diagram
C1K3L2
-
-
-
?
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
C1K3L2
-
Manually annotated by BRENDA team
C1K3L2
-
Manually annotated by BRENDA team
C1K3L2
-
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
C1K3L2
-
Manually annotated by BRENDA team
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
75000
C1K3L2
determined by SDS-PAGE and Western Blot analysis
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
cell extracts are prepared
C1K3L2
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
full-length CMAH cDNA is cloned from umbilical cord blood mononuclear cells and HeLa cells into the pGEM-T easy vector for sequencing, GFP- or FLAG-tagged constructs are prepared using the vectors pFLAG-CMV-2 and pEGFP-C2/pEGFP-C1
C1K3L2
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
medicine
C1K3L2
the results pinpoint the crucial need for totally xenofree culturing and expansion conditions for human therapeutic stem cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nystedt, J.; Anderson, H.; Hirvonen, T.; Impola, U.; Jaatinen, T.; Heiskanen, A.; Blomquist, M.; Satomaa, T.; Natunen, J.; Saarinen, J.; Lehenkari, P.; Valmu, L.; Laine, J.
Human CMP-N-acetylneuraminic acid hydroxylase (CMAH) is a novel stem cell marker linked to stem cell-specific mechanisms
Stem Cells
28
258-267
2010
Homo sapiens (C1K3L2), Homo sapiens
Manually annotated by BRENDA team