We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Information on EC 1.14.15.9 - spheroidene monooxygenase for references in articles please use BRENDA:EC1.14.15.9Word Map on EC 1.14.15.9
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Specify your search results
The enzyme appears in viruses and cellular organisms
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
spheroidene monooxygenase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
2,2-dihydroxyspheroidene = spheroiden-2-one + H2O
(1c), spontaneous
-
-
-
2,2-dihydroxyspirilloxanthin = 2-oxospirilloxanthin + H2O
(2c), spontaneous; (3c), spontaneous
-
-
-
2-hydroxyspheroidene + reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ = 2,2-dihydroxyspheroidene + oxidized ferredoxin [iron-sulfur] cluster + H2O
(1b)
-
-
-
2-hydroxyspirilloxanthin + reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ = 2,2-dihydroxyspirilloxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
(2b)
-
-
-
2-oxospirilloxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 O2 = 2,2'-dioxospirilloxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + 3 H2O
(3)
-
-
-
2-oxospirilloxanthin + reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ = 2'-hydroxy-2-oxospirilloxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
(3a); (3b)
-
-
-
spheroidene + 2 reduced ferredoxin [iron-sulfur] cluster + 2 O2 = spheroiden-2-one + 2 oxidized ferredoxin [iron-sulfur] cluster + 3 H2O
spheroidene + reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ = 2-hydroxyspheroidene + oxidized ferredoxin [iron-sulfur] cluster + H2O
(1a)
-
-
-
spirilloxanthin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 O2 = 2-oxospirilloxanthin + 2 oxidized ferredoxin [iron-sulfur] cluster + 3 H2O
(2)
-
-
-
spirilloxanthin + reduced ferredoxin [iron-sulfur] cluster + O2 + 2 H+ = 2-hydroxyspirilloxanthin + oxidized ferredoxin [iron-sulfur] cluster + H2O
(2a)
-
-
-
spheroidene + 2 reduced ferredoxin [iron-sulfur] cluster + 2 O2 = spheroiden-2-one + 2 oxidized ferredoxin [iron-sulfur] cluster + 3 H2O
(1)
-
spheroidene + 2 reduced ferredoxin [iron-sulfur] cluster + 2 O2 = spheroiden-2-one + 2 oxidized ferredoxin [iron-sulfur] cluster + 3 H2O
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
spheroidene and spheroidenone biosynthesis
-
-
spirilloxanthin and 2,2'-diketo-spirilloxanthin biosynthesis
-
-
Carotenoid biosynthesis
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
spheroidene,reduced-ferredoxin:oxygen oxidoreductase (spheroiden-2-one-forming)
The enzyme is involved in spheroidenone biosynthesis and in 2,2'-dioxospirilloxanthin biosynthesis. The enzyme from Rhodobacter sphaeroides contains heme at its active site [1].
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
acyclic carotenoid 2-ketolase
acyclic carotenoid 2-ketolase
-
-
acyclic carotenoid 2-ketolase
-
-
CrtA
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
physiological function
-
the enzyme is involved in spheroidene biosynthesis pathway and spirilloxanthin biosynthesis pathway. Methylated carotenoids, such as spheroidene and spirilloxanthin, function as accessory pigments in the light-harvesting and reaction-center complexes of purple bacteria
malfunction
-
the combined manipulation of crtF and crtA allows new carotenoids to be produced and broadenes the diversity of structurally different carotenoids synthesized by Rubriovivax gelatinosus
malfunction
-
crtA-deleted mutants of Rhodovulum sulfidophilum did not produce spheroiden-2-one and 1-demethylspheroidenone. The ratio of demethylspheroidene to spheroidene increased with exposure to light
malfunction
-
a Tn5.7 mutation in the 3' region of the crtA yields a bacteriochllorophyll-minus phenotype, while a 5'-insertion affects only carotenoid biosynthesis
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
1'-hydroxy-1-demethylspheroidene + reduced ferredoxin + O2
1'-hydroxy-1-demethylspheroiden-2-one + oxidized ferredoxin + H2O
1'-hydroxyspheroidene + reduced ferredoxin + O2
1'-hydroxyspheroiden-2-one + oxidized ferredoxin + H2O
1,1'-dihydroxy-3,4-didehydrolycopene + 2 reduced ferredoxin + 2 O2
2,2'-dioxo-1,1'-dihydroxy-3,4-didehydrolycopene + 2 oxidized ferredoxin + 2 H2O
-
-
-
-
?
1-hydroxy-3,4-didehydrolycopene + reduced ferredoxin + O2
2-oxo-1-hydroxy-3,4-didehydrolycopene + oxidized ferredoxin + H2O
-
-
-
-
?
2-hydroxyspheroidene + reduced ferredoxin + O2
spheroiden-2-one + oxidized ferredoxin + H2O
3,4,3',4'-tetradehydrolycopene + reduced ferredoxin + O2
1,1'-dihydroxy-3,4,3',4'-tetradehydrolycopen-2-one + oxidized ferredoxin + H2O
-
-
-
-
?
3,4,3',4'-tetradehydrolycopene + reduced ferredoxin + O2
1-hydroxy-3,4,3',4'-tetradehydrolycopen-2-one + oxidized ferredoxin + H2O
-
-
-
-
?
3,4-didehydrolycopene + reduced ferredoxin + O2
1-hydroxy-3,4-didehydrolycopen-2-one + oxidized ferredoxin + H2O
-
-
-
-
?
spheroidene + reduced ferredoxin + O2
spheroiden-2-one + oxidized ferredoxin + H2O
spheroidene + reduced ferredoxin + O2
spheroidenone + oxidized ferredoxin + H2O
-
-
-
-
?
spirilloxanthin + 2 reduced ferredoxin + 2 O2
2,2'-dioxosprilloxanthin + 2 oxidized ferredoxin + 2 H2O
spirilloxanthin + reduced ferredoxin + O2
2-oxospirilloxanthin + oxidized ferredoxin + H2O
1'-hydroxy-1-demethylspheroidene + reduced ferredoxin + O2
1'-hydroxy-1-demethylspheroiden-2-one + oxidized ferredoxin + H2O
-
conversion rate: 28%
-
-
?
1'-hydroxy-1-demethylspheroidene + reduced ferredoxin + O2
1'-hydroxy-1-demethylspheroiden-2-one + oxidized ferredoxin + H2O
-
conversion rate: 37%
-
-
?
1'-hydroxyspheroidene + reduced ferredoxin + O2
1'-hydroxyspheroiden-2-one + oxidized ferredoxin + H2O
-
conversion rate: 41%
-
-
?
1'-hydroxyspheroidene + reduced ferredoxin + O2
1'-hydroxyspheroiden-2-one + oxidized ferredoxin + H2O
-
conversion rate: 76%
-
-
?
2-hydroxyspheroidene + reduced ferredoxin + O2
spheroiden-2-one + oxidized ferredoxin + H2O
-
-
-
-
?
2-hydroxyspheroidene + reduced ferredoxin + O2
spheroiden-2-one + oxidized ferredoxin + H2O
-
-
-
-
?
spheroidene + reduced ferredoxin + O2
spheroiden-2-one + oxidized ferredoxin + H2O
-
conversion rate: 36%
-
-
?
spheroidene + reduced ferredoxin + O2
spheroiden-2-one + oxidized ferredoxin + H2O
-
conversion rate: 36%
-
-
?
spheroidene + reduced ferredoxin + O2
spheroiden-2-one + oxidized ferredoxin + H2O
-
conversion rate: 68%
-
-
?
spirilloxanthin + 2 reduced ferredoxin + 2 O2
2,2'-dioxosprilloxanthin + 2 oxidized ferredoxin + 2 H2O
-
conversion rate: 41%
-
-
?
spirilloxanthin + 2 reduced ferredoxin + 2 O2
2,2'-dioxosprilloxanthin + 2 oxidized ferredoxin + 2 H2O
-
-
-
-
?
spirilloxanthin + 2 reduced ferredoxin + 2 O2
2,2'-dioxosprilloxanthin + 2 oxidized ferredoxin + 2 H2O
-
conversion rate: 43%
-
-
?
spirilloxanthin + reduced ferredoxin + O2
2-oxospirilloxanthin + oxidized ferredoxin + H2O
-
conversion rate: 59%
-
-
?
spirilloxanthin + reduced ferredoxin + O2
2-oxospirilloxanthin + oxidized ferredoxin + H2O
-
-
-
-
?
spirilloxanthin + reduced ferredoxin + O2
2-oxospirilloxanthin + oxidized ferredoxin + H2O
-
conversion rate: 59%
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
spheroidene + reduced ferredoxin + O2
spheroiden-2-one + oxidized ferredoxin + H2O
-
conversion rate: 36%
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
heme
-
CrtA has a 5-coordinated heme at its active site
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.0101 - 0.0155
1'-hydroxyspheroidene
0.007 - 0.0191
spheroidene
0.009 - 0.0181
spirilloxanthin
0.0101
1'-hydroxyspheroidene
-
pH 7.8, 28°C
0.0155
1'-hydroxyspheroidene
-
pH 7.8, 28°C
0.007
spheroidene
-
pH 7.8, 28°C
0.0191
spheroidene
-
pH 7.8, 28°C
0.009
spirilloxanthin
-
pH 7.8, 28°C, formation of 2,2'-dioxospririlloxanthin
0.0136
spirilloxanthin
-
pH 7.8, 28°C, formation of 2,2'-diketospririlloxanthin
0.0169
spirilloxanthin
-
pH 7.8, 28°C, formation of 2-ketospririlloxanthin
0.0181
spirilloxanthin
-
pH 7.8, 28°C, formation of 2-oxospririlloxanthin
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
7.8
-
assay at
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
28
-
assay at
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
32000
-
x * 32000, SDS-PAGE
65000
-
x * 65000, calculated from sequence
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
?
-
x * 32000, SDS-PAGE
?
-
x * 65000, calculated from sequence
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
expression in Escherichia coli
expression in Escherichia coli. Coexpression of CrtA with acyclic C40 carotenoid pathways can introduce a keto group at the C(2,2') position of unnatural substrates that do not exhibit a C(3,4) double bond. The complete conversion of tetradehydrolycopene to phillipsiaxanthin observed suggests it is a favorable substrate for CrtA activity when compared to the incomplete conversion of lycopene to lycopene-2-one in the presence of CrtA
-
expression in Escherichia coli
-
expression in Escherichia coli
-
expression in Escherichia coli
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
analysis
-
construction of a simple and cost-effective sensor independent from specific reagents and instruments for monitoring qualities of marine aquacultural fields. A sensor strain consisting of a crtA deleted host strain of Rhodovulum sulfidophilum, in which the CrtA gene is reintroduced downstream of the promoter for the dimethyl sulfide dehydrogenase (Ddh) gene cluster, is constructed to detect dimethyl sulfide in the nursery of lavers based on the conversion of the carotenoid colors
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CRTA_RHOCB
Rhodobacter capsulatus (strain ATCC BAA-309 / NBRC 16581 / SB1003)
241
27004
Swiss-Prot
A0A238JQK8_9RHOB
240
26718
TrEMBL
A0A0N7J8D2_9BURK
292
31191
TrEMBL
A0A238LBV0_9RHOB
234
25298
TrEMBL
A0A2R8A9W5_9RHOB
256
28709
TrEMBL
A0A0P0MEH1_9BURK
270
29620
TrEMBL
A0A0P1EZC4_9RHOB
237
25941
TrEMBL
A0A1V0RUB6_9RHOB
262
28773
TrEMBL
A0A0D1CJK5_9RHOB
209
22754
TrEMBL
A0A136P6N6_9BACT
229
25857
TrEMBL
A0A0M6YCG5_9RHOB
237
26485
TrEMBL
A0A0E3V0Y2_9BURK
230
25009
TrEMBL
A0A1Y0EHI7_9RHOB
245
26318
TrEMBL
A0A1Y5S3Z2_9RHOB
235
26171
TrEMBL
A0A2R8BPC5_9RHOB
215
23735
TrEMBL
A0A1L9NUN2_9RHOB
251
28171
TrEMBL
A0A0S2I324_9BACT
232
27252
TrEMBL
A0A1Y2NI83_STRPT
232
25090
TrEMBL
A0A0L6CWP3_9RHOB
212
23507
TrEMBL
A0A1Y5S697_9RHOB
234
25736
TrEMBL
A0A0U1NP75_9RHOB
211
23021
TrEMBL
A0A222TPY2_GORRU
237
26288
TrEMBL
A0A2S2DUU5_9BACT
224
26018
TrEMBL
A0A1X6ZNA0_9RHOB
235
26662
TrEMBL
A0A177HBU9_9RHOB
212
23578
TrEMBL
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Lee, P.C.; Holtzapple, E.; Schmidt-Dannert, C.
Novel activity of Rhodobacter sphaeroides spheroidene monooxygenase CrtA expressed in Escherichia coli
Appl. Environ. Microbiol.
76
7328-7331
2010
Rhodobacter sphaeroides
brenda
Maeda, I.; Yamashiro, H.; Yoshioka, D.; Onodera, M.; Ueda, S.; Kawase, M.; Miyasaka, H.; Yagi, K.
Colorimetric dimethyl sulfide sensor using Rhodovulum sulfidophilum cells based on intrinsic pigment conversion by CrtA
Appl. Microbiol. Biotechnol.
70
397-402
2006
Rhodovulum sulfidophilum
brenda
Pinta, V.; Ouchane, S.; Picaud, M.; Takaichi, S.; Astier, C.; Reiss-Husson, F.
Characterization of unusual hydroxy- and ketocarotenoids in Rubrivivax gelatinosus: involvement of enzyme CrtF or CrtA
Arch. Microbiol.
179
354-362
2003
Rubrivivax gelatinosus
brenda
Gerjets, T.; Steiger, S.; Sandmann, G.
Catalytic properties of the expressed acyclic carotenoid 2-ketolases from Rhodobacter capsulatus and Rubrivivax gelatinosus
Biochim. Biophys. Acta
1791
125-131
2008
Rhodobacter capsulatus, Rubrivivax gelatinosus
brenda
Lee, P.C.; Momen, A.Z.; Mijts, B.N.; Schmidt-Dannert, C.
Biosynthesis of structurally novel carotenoids in Escherichia coli
Chem. Biol.
10
453-462
2003
Rhodobacter capsulatus
brenda
Maeda, I.; Yamashiro, H.; Yoshioka, D
Onodera, M.; Ueda, S.; Miyasaka, H.; Umeda, F.; Kawase, M.; Takaichi, S.; Yagi, K.: Unusual accumulation of demethylspheroidene in anaerobic-phototrophic growth of crtA-deleted mutants of Rhodovulum sulfidophilum
Curr. Microbiol.
51
193-197
2005
Rhodovulum sulfidophilum
brenda
Armstrong, G.A.; Schmidt, A.; Sandmann, G.; Hearst, J.E.
Genetic and biochemical characterization of carotenoid biosynthesis mutants of Rhodobacter capsulatus
J. Biol. Chem.
265
8329-8338
1990
Rhodobacter capsulatus
brenda
Armstrong, G.A.; Alberti, M.; Leach, F.; Hearst, J.E.
Nucleotide sequence, organization, and nature of the protein products of the carotenoid biosynthesis gene cluster of Rhodobacter capsulatus
Mol. Gen. Genet.
216
254-268
1989
Rhodobacter capsulatus
brenda
html completed