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Information on EC 1.14.15.3 - alkane 1-monooxygenase and Organism(s) Arabidopsis thaliana and UniProt Accession P48422

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IUBMB Comments
Some enzymes in this group are heme-thiolate proteins (P-450). Also hydroxylates fatty acids in the omega-position.
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This record set is specific for:
Arabidopsis thaliana
UNIPROT: P48422
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Word Map
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
+
reduced rubredoxin
+
+
2
=
+
oxidized rubredoxin
+
Synonyms
cyp4a, cyp4a11, methane monooxygenase, alkane hydroxylase, omega-hydroxylase, 1-hydroxylase, cyp4a14, cyp4a10, alkane monooxygenase, cyp153, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fatty acid omega-hydroxylase
-
1-hydroxylase
-
-
-
-
alkane 1-hydrolase
-
-
-
-
alkane hydroxylase
-
-
-
-
alkane monooxygenase
-
-
-
-
CYP4AII
-
-
-
-
CYPIVA1
-
-
-
-
CYPIVA11
-
-
-
-
CYPIVA2
-
-
-
-
CYPIVA3
-
-
-
-
CYPIVA5
-
-
-
-
CYPIVA6
-
-
-
-
CYPIVA7
-
-
-
-
FA omega-hydroxylase
-
-
fatty acid omega-hydrolase
-
-
-
-
Fatty acid omega-hydroxylase
Lauric acid omega-hydroxylase
-
-
-
-
omega-hydrolase
-
-
-
-
P-450 HK omega
-
-
-
-
P450-HL-omega
-
-
-
-
P452
-
-
-
-
very long chain fatty acid hydroxylase
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
-
-
-
-
reduction
-
-
-
-
hydroxylation
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
alkane,reduced-rubredoxin:oxygen 1-oxidoreductase
Some enzymes in this group are heme-thiolate proteins (P-450). Also hydroxylates fatty acids in the omega-position.
CAS REGISTRY NUMBER
COMMENTARY hide
9059-16-9
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
suberin + NADPH + H+ + O2
? + NADP+ + H2O
show the reaction diagram
-
-
-
?
fatty acid + NAD(P)H + H+ + O2
omega-hydroxy fatty acid + NAD(P)+ + H2O
show the reaction diagram
-
-
-
-
?
lauric acid + NAD(P)H + H+ + O2
12-hydroxydodecanoic acid + NAD(P)+ + H2O
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
-
non-heme iron enzyme
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
strong root specificity and a localized expression in the root endodermis, high expression levels in all three root zones from the tip to the base
Manually annotated by BRENDA team
highly expressed
Manually annotated by BRENDA team
elevated expression
Manually annotated by BRENDA team
additional information
no detectable CYP86A1 transcript levels in above-ground organs such as leaves, stems, flowers, and siliques
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
transgenic expression of CYP86A1 fused to GFP distributes CYP86A1 to the endoplasmic reticulum, indicating that suberin monomer biosynthesis takes place in this sub-cellular compartment before intermediates are exported in the apoplast
Manually annotated by BRENDA team
guard cells in the epidermis
Manually annotated by BRENDA team
predominant localization
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
is a key enzyme for aliphatic root suberin biosynthesis. In contrast to wild-type, no CYP86A1 transcript in total RNA from horst-1 seedlings (carries the T-DNA insertion in the first exon of CYP86A1) and horst-2 seedlings (T-DNA insertion is located in the second exon). Complemented CYP86A1-transformed horst-1 plants reveal a quantitative and qualitative aliphatic suberin composition similar to the wild-type
malfunction
physiological function
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
C86A1_ARATH
513
1
58553
Swiss-Prot
other Location (Reliability: 1)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
in the insertion mutant lines cyp86b1-1 and cyp86b1-2 ralph1 (root aliphatic plant hydroxylase1) and ralph2, respectively, transcript levels are at the detection limit. Transgenic lines ralph3 and ralph4 expressing RNAi constructs show significant down-regulation of CYP86B1 transcript levels
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
the 1.4 kb putative promoter region upstream of the predicted CYP86A1 start codon fused in frame to the beta-glucuronidase gene and used to transform wild-type Arabidopsis under the control of the native promoter. Transgenic expression of CYP86A1 fused to GFP, CYP86A1:GFP heterologously and transiently expressed under the control of the CaMV35S-promoter in the epidermis of Nicotiana benthamiana leaves
CYP86B1-YFP fusion protein expressed under the control of the 35S promoter in a transient expression assay in Nicotiana benthamiana leaves. ProCYP86B1::GUS transgenic plants
expressed in Saccharomyces cerevisiae strain WAT11
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Coon, M.J.
Omega oxygenases: Nonheme-iron enzymes and P450 cytochromes
Biochem. Biophys. Res. Commun.
338
378-385
2005
Arabidopsis thaliana, Candida tropicalis, Homo sapiens, Nicotiana tabacum, Pseudomonas sp., Rattus norvegicus, Vicia sativa, Zea mays
Manually annotated by BRENDA team
Benveniste, I.; Saito, T.; Wang, Y.; Kandel, S.; Huang, H.; Pinot, F.; Kahn, R.A.; Salauen, J.; Shimoji, M.
Evolutionary relationship and substrate specificity of Arabidopsis thaliana fatty acid omega-hydroxylase
Plant Sci.
170
326-338
2006
Arabidopsis thaliana
Manually annotated by BRENDA team
Hoefer, R.; Briesen, I.; Beck, M.; Pinot, F.; Schreiber, L.; Franke, R.
The Arabidopsis cytochrome P450 CYP86A1 encodes a fatty acid omega-hydroxylase involved in suberin monomer biosynthesis
J. Exp. Bot.
59
2347-2360
2008
Arabidopsis thaliana (P48422)
Manually annotated by BRENDA team
Compagnon, V.; Diehl, P.; Benveniste, I.; Meyer, D.; Schaller, H.; Schreiber, L.; Franke, R.; Pinot, F.
CYP86B1 is required for very long chain omega-hydroxyacid and alpha, omega -dicarboxylic acid synthesis in root and seed suberin polyester
Plant Physiol.
150
1831-1843
2009
Arabidopsis thaliana (Q9FMY1)
Manually annotated by BRENDA team