Any feedback?
Please rate this page
(enzyme.php)
(0/150)

BRENDA support

BRENDA Home
show all | hide all No of entries

Information on EC 1.14.14.94 - leukotriene-B4 20-monooxygenase and Organism(s) Mus musculus and UniProt Accession Q9EP75

for references in articles please use BRENDA:EC1.14.14.94
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
IUBMB Comments
A cytochrome P-450 (heme-thiolate) protein found in mammals.
Specify your search results
Select one or more organisms in this record: ?
This record set is specific for:
Mus musculus
UNIPROT: Q9EP75
Show additional data
Do not include text mining results
Include (text mining) results
Include results (AMENDA + additional results, but less precise)
Word Map
  • 1.14.14.94
  • warfarin
  • vkorc1
  • cyp2c9
  • anticoagulant
  • arachidonic
  • dosing
  • pharmacogenetic
  • 20-hete
  • 20-hydroxyeicosatetraenoic
  • cyp4fs
  • cyp4a11
  • omega-hydroxylation
  • epoxide
  • eicosanoids
  • cyp3a5
  • interindividual
  • acenocoumarol
  • pharmacogenomics
  • thromboembolic
  • non-genetic
  • cyp1b1
  • omega-oxidation
  • 17-octadecynoic
  • epoxyeicosatrienoic
  • pharmacogenes
  • genotype-guided
  • lipoxins
The taxonomic range for the selected organisms is: Mus musculus
The enzyme appears in selected viruses and cellular organisms
Synonyms
cyp4f2, cyp4f3, cyp4f12, cyp4f11, cyp4f8, cyp4f3b, ltb4 omega-hydroxylase, cytochrome p450 4f2, cyp4f3a, cyp4f4, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CYP4F13
-
-
CYP4F14
-
-
CYP4F15
-
-
CYP4F16
-
-
CYP4F17
-
-
CYP4F18
-
-
CYP4F37
-
-
CYP4F39
-
-
CYP4F40
-
-
CYPIVF2
-
-
-
-
CYPIVF3
-
-
-
-
cytochrome P-450 4F18
-
-
Cytochrome P450-LTB-omega
-
-
-
-
leukotriene B4 20-hydroxylase
-
-
-
-
leukotriene B4 omega-1/omega-2 hydroxylase
-
-
Leukotriene-B4 20-monooxygenase
-
-
-
-
leukotriene-B4 omega-hydroxylase
-
-
-
-
LTB4 20-hydroxylase
-
-
-
-
LTB4 hydroxylase
-
-
LTB4 omega-hydroxylase
-
-
-
-
oxygenase, leukotriene B4 20-mono-
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
reduction
-
-
-
-
omega-oxidation
-
-
-
-
PATHWAY SOURCE
PATHWAYS
-
-, -
SYSTEMATIC NAME
IUBMB Comments
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (20-hydroxylating)
A cytochrome P-450 (heme-thiolate) protein found in mammals.
CAS REGISTRY NUMBER
COMMENTARY hide
90119-11-2
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
leukotriene B4 + [reduced NADPH-hemoprotein reductase] + O2
20-hydroxy-leukotriene B4 + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
leukotriene B4 is identical with (6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate
-
-
?
prostaglandin A1 + [reduced NADPH-hemoprotein reductase] + O2
20-hydroxy-prostaglandin A1 + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
recombinant CYP4F14
-
-
?
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + [reduced NADPH-hemoprotein reductase] + O2
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(6Z,8E,10E,14Z)-(5S,12R)-5,12-dihydroxyicosa-6,8,10,14-tetraenoate + [reduced NADPH-hemoprotein reductase] + O2
(6Z,8E,10E,14Z)-(5S,12R)-5,12,20-trihydroxyicosa-6,8,10,14-tetraenoate + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
-
LTB4 inactivation, in vivo inhibition of CYP4F18 results in a marked increase in calcium flux and a 220% increases in the chemotactic response of mouse polymorphonuclear leukocytes to LTB4
-
-
?
additional information
?
-
-
CYP4F18 is a critical protein in the regulation of LTB4 metabolism, the enzyme is active in down-regulation of responses to leukotriene B4, i.e. LTB4, leukotriene B4 is a potent chemoattractant for polymorphonuclear leukocytes and other cells, overview
-
-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
all-trans-retinoic acid
-
after 6 days of retinoid exposure, cutaneous transcripts arising from Cyp4f13, 4f14, and 4f37 are down-regulated. No changes in transcript abundance for the Cyp4f16, 4f17, and 4f18 genes
additional information
-
in vivo inhibition of CYP4F18 results in a marked increase in calcium flux and a 220% increase in the chemotactic response of mouse PMN to LTB4
-
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
all-trans-retinoic acid
-
after 6 days of retinoid exposure, cutaneous transcripts arising from Cyp4f39 are up-regulated. No changes in transcript abundance for the Cyp4f16, 4f17, and 4f18 genes
additional information
-
CYP4F18 expression is induced in bone marrow-derived dendritic cells by bacterial lipopolysaccharide, a ligand for TLR4, and by poly(I-C), a ligand for TLR3
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
Uniprot
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
CYP4F14
Manually annotated by BRENDA team
-
bone marrow-derived, in vitro differentiation
Manually annotated by BRENDA team
-
polymorphonuclear
Manually annotated by BRENDA team
-
transcript abundance is highest for Cyp4f13 and 4f16 genes, lowest for Cyp4f14, 4f17, and 4f37, and intermediate for Cyp4f18 and 4f39. Transcripts arising from Cyp4f15 are highly variable, ranging from intermediate to undetectable levels in individual mice. Transcripts arising from Cyp4f40 are present, but levels are too low to measure reliably in most mice
Manually annotated by BRENDA team
additional information
-
tissue-specific expression, expression analysis of CYP4F18 in different cell types, tissue distribution, overview
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
from full-thickness skin
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CP4FE_MOUSE
524
0
59800
Swiss-Prot
Secretory Pathway (Reliability: 3)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
59800
CYP4F14, predicted from gene sequence
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
CYP4F14, expression in yeast cells
gene Cyp4f18, expression analysis of CYP4F18 in different cell types, recombinant expression of the enzyme in Spodoptera frugiperda Sf9 cell microsomes via baculovirus transfection system
-
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
after 6 days of retinoid exposure, cutaneous transcripts arising from Cyp4f13, 4f14, and 4f37 are down-regulated
-
after 6 days of retinoid exposure, cutaneous transcripts arising from Cyp4f39 are up-regulated
-
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Kikuta, Y.; Kasyu, H.; Kusunose, E.; Kusunose, M.
Expression and catalytic activity of mouse leukotriene B4 omega-hydroxylase, CYP4F14
Arch. Biochem. Biophys.
383
225-232
2000
Mus musculus (Q9EP75), Mus musculus
Manually annotated by BRENDA team
Christmas, P.; Tolentino, K.; Primo, V.; Berry, K.Z.; Murphy, R.C.; Chen, M.; Lee, D.M.; Soberman, R.J.
Cytochrome P-450 4F18 is the leukotriene B4 omega-1/omega-2 hydroxylase in mouse polymorphonuclear leukocytes: identification as the functional orthologue of human polymorphonuclear leukocyte CYP4F3A in the down-regulation of responses to LTB4
J. Biol. Chem.
281
7189-7196
2006
Mus musculus
Manually annotated by BRENDA team
Du, L.; Yin, H.; Morrow, J.D.; Strobel, H.W.; Keeney, D.S.
20-Hydroxylation is the CYP-dependent and retinoid-inducible leukotriene B4 inactivation pathway in human and mouse skin cells
Arch. Biochem. Biophys.
484
80-86
2009
Homo sapiens, Mus musculus, Mus musculus CD-1
Manually annotated by BRENDA team