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Information on EC 1.14.14.76 - ent-isokaurene C2/C3-hydroxylase and Organism(s) Oryza sativa and UniProt Accession A3A871

for references in articles please use BRENDA:EC1.14.14.76
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IUBMB Comments
This cytochrome P-450 (heme thiolate) enzyme has been characterized from the plant Oryza sativa (rice). It may be involved in production of oryzadione.
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This record set is specific for:
Oryza sativa
UNIPROT: A3A871
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The taxonomic range for the selected organisms is: Oryza sativa
The enzyme appears in selected viruses and cellular organisms
Synonyms
cyp71z6, os03g0594100, ent-isokaurene c2-hydroxylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CYP71Z6
-
-
ent-isokaurene C2-hydroxylase
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
hydroxylation
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-
SYSTEMATIC NAME
IUBMB Comments
ent-isokaurene,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (ent-isokaurene-2beta,3beta-diol forming)
This cytochrome P-450 (heme thiolate) enzyme has been characterized from the plant Oryza sativa (rice). It may be involved in production of oryzadione.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
ent-isokaurene + [reduced NADPH-hemoprotein reductase] + O2
ent-2alpha-hydroxyisokaurene + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
-
-
-
-
ir
additional information
?
-
-
the enzyme does not react with ent-cassadiene
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.012
ent-isokaurene
-
pH and temperature not specified in the publication
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.01
ent-isokaurene
-
pH and temperature not specified in the publication
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
-
the enzyme acts as a C2-hydroxylase involved in a latter step of phytocassane biosynthesis
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli C41 cells
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Wu, Y.; Hillwig, M.L.; Wang, Q.; Peters, R.J.
Parsing a multifunctional biosynthetic gene cluster from rice: Biochemical characterization of CYP71Z6 & 7
FEBS Lett.
585
3446-3451
2011
Oryza sativa
Manually annotated by BRENDA team