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Information on EC 1.14.14.173 - 2,4,6-trichlorophenol monooxygenase
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1.14.14.173 2,4,6-trichlorophenol monooxygenaseIUBMB Comments
The enzyme, characterized from a number of bacterial species, participates in the degradation of 2,4,6-trichlorophenol, a compound that has been used for decades as a wood preservative. The enzyme is a multifunctional flavin-dependent monooxygenase that displaces two chlorine atoms by attacking two different positions in the substrate. Each reaction catalysed by the enzyme displaces a single chlorine and results in formation of a dione, which must be reduced by FADH2 before the monooxygenase could catalyse the second reaction. The large amount of FADH2 that is required is generated by a dedicated flavin reductase (TcpB). cf. EC 1.14.14.172, 3,5,6-trichloropyridin-2-ol monooxygenase.
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Word Map
- 1.14.14.173
- tcpa
- cupriavidus
-
dechlorination
- flavin
-
dehalogenation
- 2,4-dichlorophenol
-
1,2-dioxygenase
- chlorophenols
- ralstonia
- eutropha
- maleylacetate
The expected taxonomic range for this enzyme is: Cupriavidus
Reaction Schemes
Synonyms
2,4,6-trichlorophenol monooxygenase, 2,4,6-tcp monooxygenase, tcp-4-monooxygenase, 2,4,6-trichlorophenol-4-monooxygenase, 2,4,6-tcp 4-monooxygenase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
2,4,6-TCP 4-monooxygenase
2,4,6-TCP monooxygenase
2,4,6-trichlorophenol 4-monooxygenase
2,4,6-trichlorophenol-4-monooxygenase
TCP-4-monooxygenase
tcpA
tcpA
-
-
-
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2,4,6-trichlorophenol + FADH2 + O2 = 2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
(1)
-
-
-
2,6-dichlorohydroquinone + FADH2 + O2 = 2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
(2)
-
-
-
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PATHWAY SOURCE
PATHWAYS
-
-
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SYSTEMATIC NAME
IUBMB Comments
2,4,6-trichlorophenol,FADH2:oxygen oxidoreductase (dechlorinating)
The enzyme, characterized from a number of bacterial species, participates in the degradation of 2,4,6-trichlorophenol, a compound that has been used for decades as a wood preservative. The enzyme is a multifunctional flavin-dependent monooxygenase that displaces two chlorine atoms by attacking two different positions in the substrate. Each reaction catalysed by the enzyme displaces a single chlorine and results in formation of a dione, which must be reduced by FADH2 before the monooxygenase could catalyse the second reaction. The large amount of FADH2 that is required is generated by a dedicated flavin reductase (TcpB). cf. EC 1.14.14.172, 3,5,6-trichloropyridin-2-ol monooxygenase.
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2,3-dichlorophenol + FADH2 + O2
2,3-dichlorohydroquinone + FAD + H2O
-
-
-
-
?
2,4,6-tribromophenol + FADH2 + O2
?
Cupriavidus sp. GP1
-
57% activity compared to 2,4,6-trichlorophenol
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
?
-
94% activity compared to 2,4-dichlorophenol
-
-
?
2,4,6-triiodophenol + FADH2 + O2
?
Cupriavidus sp. GP1
-
50% activity compared to 2,4,6-trichlorophenol
-
-
?
2,4-dichlorophenol + FADH2 + O2
2-chlorobenzoquinone + Cl- + FAD + H2O
-
100% activity, 96.73% degradation in 24 h
-
-
?
2,4-dichlorophenol + FADH2 + O2
?
Cupriavidus sp. GP1
-
80% activity compared to 2,4,6-trichlorophenol
-
-
?
2,5-dichlorophenol + FADH2 + O2
2,5-dichlorohydroquinone + FAD + H2O
-
-
-
-
?
2,6-dichlorohydroquinone + FADH2 + O2
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
2,6-dichlorophenol + FADH2 + O2
?
Cupriavidus sp. GP1
-
67% activity compared to 2,4,6-trichlorophenol
-
-
?
2-bromo-4-chlorophenol + FADH2 + O2
2-bromobenzoquinone + Cl- + FAD + H2O
-
87.31% degradation in 24 h
-
-
?
2-chloro-4-bromophenol + FADH2 + O2
2-chlorobenzoquinone + Br- + FAD + H2O
-
74.48% degradation in 24 h
-
-
?
2-chloro-4-nitrophenol + FADH2 + O2
2-chlorobenzoquinone + NO2- + FAD + H2O
-
98.37% degradation in 24 h
-
-
?
2-chlorophenol + FADH2 + O2
?
Cupriavidus sp. GP1
-
25% activity compared to 2,4,6-trichlorophenol
-
-
?
3,4,5-trichlorophenol + FADH2 + O2
?
Cupriavidus sp. GP1
-
25% activity compared to 2,4,6-trichlorophenol
-
-
?
3,4-dichlorophenol + FADH2 + O2
3-chlorobenzoquinone + Cl- + FAD + H2O
-
-
-
-
?
3,4-dichlorophenol + FADH2 + O2
?
Cupriavidus sp. GP1
-
50% activity compared to 2,4,6-trichlorophenol
-
-
?
3,5-dichlorophenol + FADH2 + O2
3,5-dichlorohydroquinone + FAD + H2O
-
-
-
-
?
4-chlorophenol + FADH2 + O2
?
Cupriavidus sp. GP1
-
32% activity compared to 2,4,6-trichlorophenol
-
-
?
2,3,4,5-tetrachlorophenol + FADH2 + O2
?
-
73% activity compared to 2,4-dichlorophenol
-
-
?
2,3,4,5-tetrachlorophenol + FADH2 + O2
?
-
73% activity compared to 2,4-dichlorophenol
-
-
?
2,3,4,5-tetrachlorophenol + FADH2 + O2
?
-
73% activity compared to 2,4-dichlorophenol
-
-
?
2,3,4,5-tetrachlorophenol + FADH2 + O2
?
Cupriavidus sp. GP1
-
33% activity compared to 2,4,6-trichlorophenol
-
-
?
2,3,4,5-tetrachlorophenol + FADH2 + O2
?
Cupriavidus sp. GP1 ATCC 49806
-
33% activity compared to 2,4,6-trichlorophenol
-
-
?
2,3,4,5-tetrachlorophenol + FADH2 + O2
?
Cupriavidus sp. GP1 DSM 6428
-
33% activity compared to 2,4,6-trichlorophenol
-
-
?
2,3,4,6-tetrachlorophenol + FADH2 + O2
?
-
80% activity compared to 2,4-dichlorophenol
-
-
?
2,3,4,6-tetrachlorophenol + FADH2 + O2
?
Cupriavidus sp. GP1
-
67% activity compared to 2,4,6-trichlorophenol
-
-
?
2,4,5-trichlorophenol + FADH2 + O2
?
-
87% activity compared to 2,4-dichlorophenol
-
-
?
2,4,5-trichlorophenol + FADH2 + O2
?
Cupriavidus sp. GP1
-
33% activity compared to 2,4,6-trichlorophenol
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
Cupriavidus sp. GP1
-
-
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
Cupriavidus sp. GP1
-
highest conversion rate
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
Cupriavidus sp. GP1 ATCC 49806
-
-
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
Cupriavidus sp. GP1 ATCC 49806
-
highest conversion rate
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
Cupriavidus sp. GP1 DSM 6428
-
-
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
Cupriavidus sp. GP1 DSM 6428
-
highest conversion rate
-
-
?
2,6-dichlorohydroquinone + FADH2 + O2
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
-
?
2,6-dichlorohydroquinone + FADH2 + O2
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
-
?
2,6-dichlorohydroquinone + FADH2 + O2
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
-
?
2,6-dichlorohydroquinone + FADH2 + O2
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
-
?
2,6-dichlorohydroquinone + FADH2 + O2
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
?
2,6-dichlorohydroquinone + FADH2 + O2
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
-
?
2,6-dichlorohydroquinone + FADH2 + O2
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
?
2,6-dichlorohydroquinone + FADH2 + O2
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
-
?
2,6-dichlorohydroquinone + FADH2 + O2
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
Cupriavidus sp. GP1
-
-
-
-
?
2,6-dichlorohydroquinone + FADH2 + O2
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
Cupriavidus sp. GP1 ATCC 49806
-
-
-
-
?
2,6-dichlorohydroquinone + FADH2 + O2
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
Cupriavidus sp. GP1 DSM 6428
-
-
-
-
?
2,6-dichlorophenol + FADH2 + O2
2,6-dichlorohydroquinone + FAD + H2O
-
-
-
-
?
2,6-dichlorophenol + FADH2 + O2
2,6-dichlorohydroquinone + FAD + H2O
-
-
-
-
?
2,6-dichlorophenol + FADH2 + O2
2,6-dichlorohydroquinone + FAD + H2O
-
-
-
-
?
2,6-dichlorophenol + FADH2 + O2
2,6-dichlorohydroquinone + FAD + H2O
Cupriavidus sp. GP1
-
9% activity compared to 2,4,6-trichlorophenol
-
-
?
additional information
?
-
-
no activity with 2-chloro-4-methylphenol and 2-chlorohydroquinone
-
-
-
additional information
?
-
-
no activity with 2-chloro-4-methylphenol and 2-chlorohydroquinone
-
-
-
additional information
?
-
-
no activity with 2-chloro-4-methylphenol and 2-chlorohydroquinone
-
-
-
additional information
?
-
no activity with riboflavin and flavin mononucleotide instead of FADH2
-
-
-
additional information
?
-
no activity with riboflavin and flavin mononucleotide instead of FADH2
-
-
-
additional information
?
-
Cupriavidus sp. GP1
-
no activity with 3-chlorophenol, 2,3-dichlorophenol, 2,5-dichlorophenol, 3,5-dichlorophenol, 2,3,4-trichlorophenol, 2,3,5-trichlorophenol, 2,3,6-trichlorophenol, 2,3,5,6-tetrachlorophenol, pentachlorophenol, 2,4,5-trimethylphenol, and 2,4,6-trinitrophenol
-
-
-
additional information
?
-
Cupriavidus sp. GP1 ATCC 49806
-
no activity with 3-chlorophenol, 2,3-dichlorophenol, 2,5-dichlorophenol, 3,5-dichlorophenol, 2,3,4-trichlorophenol, 2,3,5-trichlorophenol, 2,3,6-trichlorophenol, 2,3,5,6-tetrachlorophenol, pentachlorophenol, 2,4,5-trimethylphenol, and 2,4,6-trinitrophenol
-
-
-
additional information
?
-
Cupriavidus sp. GP1 DSM 6428
-
no activity with 3-chlorophenol, 2,3-dichlorophenol, 2,5-dichlorophenol, 3,5-dichlorophenol, 2,3,4-trichlorophenol, 2,3,5-trichlorophenol, 2,3,6-trichlorophenol, 2,3,5,6-tetrachlorophenol, pentachlorophenol, 2,4,5-trimethylphenol, and 2,4,6-trinitrophenol
-
-
-
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2,6-dichlorohydroquinone + FADH2 + O2
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
Cupriavidus sp. GP1
-
-
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
Cupriavidus sp. GP1 ATCC 49806
-
-
-
-
?
2,4,6-trichlorophenol + FADH2 + O2
2,6-dichloro-1,4-benzoquinone + Cl- + FAD + H2O
Cupriavidus sp. GP1 DSM 6428
-
-
-
-
?
2,6-dichlorohydroquinone + FADH2 + O2
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
-
?
2,6-dichlorohydroquinone + FADH2 + O2
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
-
?
2,6-dichlorohydroquinone + FADH2 + O2
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
-
?
2,6-dichlorohydroquinone + FADH2 + O2
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
-
?
2,6-dichlorohydroquinone + FADH2 + O2
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
?
2,6-dichlorohydroquinone + FADH2 + O2
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
-
?
2,6-dichlorohydroquinone + FADH2 + O2
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
?
2,6-dichlorohydroquinone + FADH2 + O2
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
-
-
-
-
?
2,6-dichlorohydroquinone + FADH2 + O2
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
Cupriavidus sp. GP1
-
-
-
-
?
2,6-dichlorohydroquinone + FADH2 + O2
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
Cupriavidus sp. GP1 ATCC 49806
-
-
-
-
?
2,6-dichlorohydroquinone + FADH2 + O2
2-chloro-6-hydroxy-1,4-benzoquinone + Cl- + FAD + H2O
Cupriavidus sp. GP1 DSM 6428
-
-
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
FAD
Cupriavidus sp. GP1
-
0.03 mM FAD leads to a 4-5fold activation of the DEAE-cellulose-purified enzyme. FAD cannot be replaced by flavin mononucleotide or riboflavin
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Fe2+
Cupriavidus sp. GP1
-
slight activation at 0.1 mM. The enzyme contains 0.2 mol of Fe per mol of the homotetrameric protein
additional information
Cupriavidus sp. GP1
-
Fe2+ (0.2 mM) and Mg2+ (0.8 mM) do not enhance activity of the purified enzyme. No influence on enzyme activity is observed for 1 mM Mg2+, Ca2+, and Co2+
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2,4,6-Trichlorophenol
Cupriavidus sp. GP1
-
at 2,4,6-trichlorophenol concentrations higher than 0.1 mM, substrate inhibition which amounts to 43% at 0.2 mM 2,4,6-trichlorophenol and to 72% at 0.33 mM 2,4,6-trichlorophenol is observed
additional information
Cupriavidus sp. GP1
-
not inhibited by menadione, metapyrone, EDTA, Tiron, 1,10-phenanthroline, KCN and sodium azide (1 mM each)
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
Cupriavidus sp. GP1
-
ATP (0.01 to 1 mM), ADP (0.01 to 0.1 mM), and coenzyme A (0.5 mM) do not enhance activity of the purified enzyme
-
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.0035
with 2,6-dichlorohydroquinone as substrate, at pH 7.0 and 33°C
6.87
Cupriavidus sp. GP1
-
after 38.2fold purification, pH and temperature not specified in the publication
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
22 - 45
66, 88, 85, 86, and 42% of the optimal activity is retained at 22, 27, 30, 37, and 45°C, respectively
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
metabolism
-
the enzyme has dual dehalogenation and denitration functions
metabolism
-
the enzyme has dual dehalogenation and denitration functions
-
metabolism
-
the enzyme has dual dehalogenation and denitration functions
-
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UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
homotetramer
monomer
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 20% (w/v) polyethylene glycol 3350 (pH 7.4) with 0.2 M potassium sodium tartrate tetrahydrate
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
dithiothreitol (0.5 mM), glycerol (10% (v/v)), sucrose (10% (w/v)), or bovine liver catalase (30 ng/ml), separately or in combinations, slightly enhance enzyme stability. No influence on stability is found for 10% (v/v) ethanol, isopropanol, or H2O2 (0.1 mM). FAD (0.03 mM) leads to a slight destabilization
Cupriavidus sp. GP1
-
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, Ni-NTA column chromatography, and phenyl agarose column chromatography
amylose resin column chromatography and Ni-NTA column chromatography
-
DEAE-cellulose column chromatography, DE52 cellulose anion-exchange chromatography, and Resource Q anion -exchange chromatography
Cupriavidus sp. GP1
-
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme fused to maltose-binding protein, expressed in Escherichia coli BL21(DE3) cells
-
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Matus, V.; Sanchez, M.A.; Martinez, M.; Gonzalez, B.
Efficient degradation of 2,4,6-trichlorophenol requires a set of catabolic genes related to tcp genes from Ralstonia eutropha JMP134(pJP4)
Appl. Environ. Microbiol.
69
7108-7115
2003
Cupriavidus necator, Cupriavidus necator JMP134(pJP4)
brenda
Hayes, R.P.; Webb, B.N.; Subramanian, A.K.; Nissen, M.; Popchock, A.; Xun, L.; Kang, C.
Structural and catalytic differences between two FADH2-dependent monooxygenases 2,4,5-TCP 4-monooxygenase (TftD) from Burkholderia cepacia AC1100 and 2,4,6-TCP 4-monooxygenase (TcpA) from Cupriavidus necator JMP134
Int. J. Mol. Sci.
13
9769-9784
2012
Cupriavidus necator (Q8KTD8), Cupriavidus necator JMP134 (Q8KTD8)
brenda
Wieser, M.; Wagner, B.; Eberspaecher, J.; Lingens, F.
Purification and characterization of 2,4,6-trichlorophenol-4-monooxygenase, a dehalogenating enzyme from Azotobacter sp. strain GP1
J. Bacteriol.
179
202-208
1997
Cupriavidus sp. GP1, Cupriavidus sp. GP1 DSM 6428, Cupriavidus sp. GP1 ATCC 49806
brenda
Louie, T.M.; Webster, C.M.; Xun, L.
Genetic and biochemical characterization of a 2,4,6-trichlorophenol degradation pathway in Ralstonia eutropha JMP134
J. Bacteriol.
184
3492-3500
2002
Cupriavidus necator (Q8KTD8), Cupriavidus necator JMP134 (Q8KTD8)
brenda
Xun, L.; Webster, C.M.
A monooxygenase catalyzes sequential dechlorinations of 2,4,6-trichlorophenol by oxidative and hydrolytic reactions
J. Biol. Chem.
279
6696-6700
2004
Cupriavidus necator, Cupriavidus necator JMP134
brenda
Fang, L.; Qin, H.; Shi, T.; Wu, X.; Li, Q.; Hua, R.
Ortho and para oxydehalogenation of dihalophenols catalyzed by the monooxygenase TcpA and NAD(P)H FAD reductase Fre
J. Hazard. Mater.
388
121787
2020
Cupriavidus nantongensis, Cupriavidus nantongensis X1T
brenda
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Release 2023.1 (January 2023)
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