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Information on EC 1.14.14.160 - zealexin A1 synthase and Organism(s) Zea mays and UniProt Accession B4FVP3

for references in articles please use BRENDA:EC1.14.14.160
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IUBMB Comments
A cytochrome P-450 (heme thiolate) enzyme characterized from maize. The enzyme sequentially oxidizes(S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1, a maize phytoalexin that provides biochemical protection against fungal infection.
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This record set is specific for:
Zea mays
UNIPROT: B4FVP3
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The taxonomic range for the selected organisms is: Zea mays
The expected taxonomic range for this enzyme is: Zea mays
Synonyms
cyp71z18, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CYP71Z18
-
CYP71Z18
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(S)-beta-macrocarpene,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (zealexin A1-forming)
A cytochrome P-450 (heme thiolate) enzyme characterized from maize. The enzyme sequentially oxidizes(S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1, a maize phytoalexin that provides biochemical protection against fungal infection.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(4S)-4-(5,5-dimethylcyclohex-1-en-1-yl)cyclohex-1-ene-1-carbaldehyde + O2 + [reduced NADPH-hemoprotein reductase]
zealexin A1 + H2O + [oxidized NADPH-hemoprotein reductase]
show the reaction diagram
the enzyme sequentially oxidizes(S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1
-
-
?
(S)-beta-macrocarpene + 3 O2 + 3 [reduced NADPH-hemoprotein reductase]
zealexin A1 + 4 H2O + 3 [oxidized NADPH-hemoprotein reductase]
show the reaction diagram
(S)-beta-macrocarpene + O2 + [reduced NADPH-hemoprotein reductase]
[(4S)-4-(5,5-dimethylcyclohex-1-en-1-yl)-cyclohex-1-en-1-yl]methanol + H2O + [oxidized NADPH-hemoprotein reductase]
show the reaction diagram
the enzyme sequentially oxidizes(S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1
-
-
?
[(4S)-4-(5,5-dimethylcyclohex-1-en-1-yl)-cyclohex-1-en-1-yl] methanol + O2 + [reduced NADPH-hemoprotein reductase]
(4S)-4-(5,5-dimethylcyclohex-1-en-1-yl)cyclohex-1-ene-1-carbaldehyde + 2 H2O + [oxidized NADPH-hemoprotein reductase]
show the reaction diagram
the enzyme sequentially oxidizes(S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-beta-macrocarpene + 3 O2 + 3 [reduced NADPH-hemoprotein reductase]
zealexin A1 + 4 H2O + 3 [oxidized NADPH-hemoprotein reductase]
show the reaction diagram
the enzyme sequentially oxidizes (S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1, a maize phytoalexin that provides biochemical protection against fungal infection, overall reaction
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P-450
a cytochrome P-450 (heme thiolate) enzyme
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the enzyme sequentially oxidizes (S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1, a maize phytoalexin that provides biochemical protection against fungal infection
physiological function
the enzyme sequentially oxidizes (S)-beta-macrocarpene via alcohol and aldehyde intermediates to form zealexin A1, a maize phytoalexin that provides biochemical protection against fungal infection
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
C71Z8_MAIZE
505
0
56021
Swiss-Prot
Secretory Pathway (Reliability: 5)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
CYP71Z18 is expressed in the C41 (DE3) OverExpress strain of Escherichia coli (Lucigen)
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
transcription is strongly induced by fungal infection
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mao, H.; Liu, J.; Ren, F.; Peters, R.J.; Wang, Q.
Characterization of CYP71Z18 indicates a role in maize zealexin biosynthesis
Phytochemistry
121
4-10
2016
Zea mays (B4FVP3), Zea mays
Manually annotated by BRENDA team