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Information on EC 1.14.14.157 - indolin-2-one monooxygenase and Organism(s) Zea mays and UniProt Accession Q43255

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IUBMB Comments
A cytochrome P-450 (heme-thiolate) protein. The enzyme is involved in the biosynthesis of protective and allelophatic benzoxazinoids in some plants, most commonly from the family of Poaceae (grasses).
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This record set is specific for:
Zea mays
UNIPROT: Q43255
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The taxonomic range for the selected organisms is: Zea mays
The expected taxonomic range for this enzyme is: Zea mays
Synonyms
BX3, EC 1.14.13.138, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
BX3
-
-
SYSTEMATIC NAME
IUBMB Comments
indolin-2-one,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (3-hydroxylating)
A cytochrome P-450 (heme-thiolate) protein. The enzyme is involved in the biosynthesis of protective and allelophatic benzoxazinoids in some plants, most commonly from the family of Poaceae (grasses).
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
indolin-2-one + [reduced NADPH-hemoprotein reductase] + O2
3-hydroxyindolin-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
-
-
-
?
benzoxazin-3-one + [reduced NADPH-hemoprotein reductase] + O2
2-hydroxy-1,4-benzoxazin-3-one + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
-
-
-
-
?
indolin-2-one + [reduced NADPH-hemoprotein reductase] + O2
3-hydroxyindolin-2-one + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
dependent on
-
cytochrome P450
-
-
-
NADPH
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.011 - 0.018
benzoxazin-3-one
0.01 - 0.017
indolin-2-one
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00194
-
recombinant enzyme, using indolin-2-one as substrate, at pH 7.5 and 25°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
-
-
-
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
C71C2_MAIZE
536
2
59783
Swiss-Prot
other Location (Reliability: 5)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae WAT11 cells
expressed in Saccharomyces cerevisiae strain WAT11 and in Escherichia coli strain BL21 (DE3)
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Frey, M.; Chomet, P.; Glawischnig, E.; Stettner, C.; Grun, S.; Winklmair, A.; Eisenreich, W.; Bacher, A.; Meeley, R.B.; Briggs, S.P.; Simcox, K.; Gierl, A.
Analysis of a chemical plant defense mechanism in grasses
Science
277
696-699
1997
Zea mays (Q43255)
Manually annotated by BRENDA team
Glawischnig, E.; Grn, S.; Frey, M.; Gierl, A.
Cytochrome P450 monooxygenases of DIBOA biosynthesis: Specificity and conservation among grasses
Phytochemistry
50
925-930
1999
Zea mays
Manually annotated by BRENDA team