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Information on EC 1.14.14.148 - angelicin synthase and Organism(s) Pastinaca sativa and UniProt Accession C0SJS3

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EC Tree
IUBMB Comments
This cytochrome P-450 (heme-thiolate) enzyme from wild parsnip is involved in the formation of angular furanocoumarins. Attacks its substrate by syn-elimination of hydrogen from C-3'.
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This record set is specific for:
Pastinaca sativa
UNIPROT: C0SJS3
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The taxonomic range for the selected organisms is: Pastinaca sativa
The enzyme appears in selected viruses and cellular organisms
Synonyms
angelicin synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
angelicin synthase
monooxygenase involved in angular furanocoumarin biosynthesis
SYSTEMATIC NAME
IUBMB Comments
(+)-columbianetin,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase
This cytochrome P-450 (heme-thiolate) enzyme from wild parsnip is involved in the formation of angular furanocoumarins. Attacks its substrate by syn-elimination of hydrogen from C-3'.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-columbianetin + [reduced NADPH-hemoprotein reductase] + O2
angelicin + acetone + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
syn-[3'D]columbianetin + [reduced NADPH-hemoprotein reductase] + O2
syn-[3'D](anti-3'-hydroxy)-columbianetin + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
-
-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(+)-columbianetin + [reduced NADPH-hemoprotein reductase] + O2
angelicin + acetone + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
the enzyme is involved in angular furanocoumarin pathway
negligible amounts of a hydroxylated columbianetin by-product
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
-
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0014 - 0.0021
(+)-columbianetin
0.0014 - 0.0038
syn-[3'D]columbianetin
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.32 - 1.9
(+)-columbianetin
0.06 - 3.9
syn-[3'D]columbianetin
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
227 - 920
(+)-columbianetin
30 - 967
syn-[3'D]columbianetin
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
fragment
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
ANGS_PASSA
478
0
54135
Swiss-Prot
Mitochondrion (Reliability: 5)
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in yeast
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Larbat, R.; Hehn, A.; Hans, J.; Schneider, S.; Jugde, H.; Schneider, B.; Matern, U.; Bourgaud, F.
Isolation and functional characterization of CYP71AJ4 encoding for the first P450 monooxygenase of angular furanocoumarin biosynthesis
J. Biol. Chem.
284
4776-4785
2009
Pastinaca sativa (C0SJS3), Pastinaca sativa
Manually annotated by BRENDA team