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Information on EC 1.14.14.121 - protopanaxadiol 6-hydroxylase and Organism(s) Panax ginseng and UniProt Accession I7C6E8

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IUBMB Comments
A cytochrome P-450 (heme-thiolate) protein isolated from the rhizomes of ginseng (Panax ginseng). Involved in the biosynthetic pathway of ginsenosides.
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Panax ginseng
UNIPROT: I7C6E8
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The taxonomic range for the selected organisms is: Panax ginseng
The enzyme appears in selected viruses and cellular organisms
Synonyms
cyp716a53v2, protopanaxadiol 6-hydroxylase, cyp6h, protopanaxatriol synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CYP716A53v2
-
CYP716A53v2
P6H
-
-
-
-
protopanaxatriol synthase
PATHWAY SOURCE
PATHWAYS
-
-
SYSTEMATIC NAME
IUBMB Comments
protopanaxadiol,[reduced NADPH-hemoprotein reductase]:oxygen oxidoreductase (6alpha-hydroxylating)
A cytochrome P-450 (heme-thiolate) protein isolated from the rhizomes of ginseng (Panax ginseng). Involved in the biosynthetic pathway of ginsenosides.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
protopanaxadiol + [reduced NADPH-hemoprotein reductase] + O2
protopanaxatriol + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
-
-
-
ir
protopanaxadiol + [reduced NADPH-hemoprotein reductase] + O2
protopanaxatriol + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
protopanaxadiol + [reduced NADPH-hemoprotein reductase] + O2
protopanaxatriol + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
-
-
-
ir
protopanaxadiol + [reduced NADPH-hemoprotein reductase] + O2
protopanaxatriol + [oxidized NADPH-hemoprotein reductase] + H2O
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
-
-
NADPH-hemoprotein reductase
A flavoprotein containing both FMN and FAD. This enzyme catalyses the transfer of electrons from NADPH, an obligatory two-electron donor, to microsomal P-450 monooxygenases, EC 1.14.14._
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
highest expression
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
protopanaxadiol 6-hydroxylase produces protopanaxatriol from protopanaxadiol, which is an important step in the formation of dammarane-type triterpene aglycones in ginseng saponin biosynthesis
metabolism
two cytochrome P450 (CYP) genes, CYP716A47 and CYP716A53v2, are critical for the production of protopanaxadiol and protopanaxatriol aglycones, respectively. CYP716A53v2 is a protopanaxadiol 6-hydroxylase that catalyzes protopanaxatriol production from protopanaxadiol in Panax ginseng, biosynthetic pathway for ginsenosides in Panax ginseng, overview
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
C7A52_PANGI
481
1
53955
Swiss-Prot
-
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
55300
x * 55300, calculated from amino acid sequence
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 55300, calculated from amino acid sequence
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Saccharomyces cerevisiae WAT21 cells
expressed in Saccharomyces cerevisiae strain GY-1
gene CYP716A53v2, recombinant overexpression in Panx ginseng roots using the Agrobacterium tumefaciens strain GV3101 transformation method, expression under control of CaMV 35S promoter, subcloning in Escherichia coli strain DH5alpha
EXPRESSION
ORGANISM
UNIPROT
LITERATURE
the enzyme is not affected by methyl jasmonate treatment
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Han, J.Y.; Hwang, H.S.; Choi, S.W.; Kim, H.J.; Choi, Y.E.
Cytochrome P450 CYP716A53v2 catalyzes the formation of protopanaxatriol from protopanaxadiol during ginsenoside biosynthesis in Panax ginseng
Plant Cell Physiol.
53
1535-1545
2012
Panax ginseng (I7C6E8), Panax ginseng
Manually annotated by BRENDA team
Dai, Z.; Wang, B.; Liu, Y.; Shi, M.; Wang, D.; Zhang, X.; Liu, T.; Huang, L.; Zhang, X.
Producing aglycons of ginsenosides in bakers yeast
Sci. Rep.
4
3698
2014
Panax ginseng (I7CT85), Panax ginseng
Manually annotated by BRENDA team
Park, S.B.; Chun, J.H.; Ban, Y.W.; Han, J.Y.; Choi, Y.E.
Alteration of Panax ginseng saponin composition by overexpression and RNA interference of the protopanaxadiol 6-hydroxylase gene (CYP716A53v2)
J. Ginseng Res.
40
47-54
2016
Panax ginseng (I7CT85), Panax ginseng
Manually annotated by BRENDA team