Information on EC 1.14.13.71 - N-methylcoclaurine 3'-monooxygenase

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The expected taxonomic range for this enzyme is: Nelumbo nucifera

EC NUMBER
COMMENTARY hide
1.14.13.71
transferred to EC 1.14.14.102
RECOMMENDED NAME
GeneOntology No.
N-methylcoclaurine 3'-monooxygenase
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Isoquinoline alkaloid biosynthesis
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Metabolic pathways
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Biosynthesis of secondary metabolites
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
isozyme 2; 2 isozymes, 2 cultivars
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
the enzyme is involved in the common pathway of benzylisoquinoline alkaloid biosynthesis and the aporphine alkaloid bisynthesis pathway in lotus, overview. A series of enzymes, norcoclaurine 6-O-methyltransferase (6OMT), coclaurineN-methyltransferase (CNMT), (S)-N-methylcoclaurine-3-hydroxylase (NMCH), and 3-hydroxy-N-methylcoclaurine 4'-O-methyltransferase(4'OMT), exhibit catalytic activity to yield the central branchpoint intermediate (S)-reticuline
physiological function
the expression of isozyme 1 is low in the two cultivars, so it may have little effect on alkaloid contents. The expression of CYP80B, NNU08355, has a positive effect on aporphine alkaloids content in lotus, but exhibits phenotypic lag, which might be because of NMCH which acts early in the benzylisoquinoline alkaloid biosynthetic pathway
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-N-methylcoclaurine + NADPH + H+ + O2
(S)-3'-hydroxy-N-methylcoclaurine + NADP+ + H2O
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
(S)-N-methylcoclaurine + NADPH + H+ + O2
(S)-3'-hydroxy-N-methylcoclaurine + NADP+ + H2O
show the reaction diagram
A0A1U7Z269
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene LOC104585940 encoding isozyme 2, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis