Information on EC 1.14.13.53 - 4'-methoxyisoflavone 2'-hydroxylase

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The expected taxonomic range for this enzyme is: Slackia sp. AUH-JLC159

EC NUMBER
COMMENTARY hide
1.14.13.53
transferred to EC 1.14.14.89
RECOMMENDED NAME
GeneOntology No.
4'-methoxyisoflavone 2'-hydroxylase
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Isoflavonoid biosynthesis
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-
Biosynthesis of secondary metabolites
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-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
daidzein + NADPH + O2
2'-hydroxydaidzein + NADP+ + H2O
show the reaction diagram
-
-
-
?
genistein + NADPH + O2
2'-hydroxygenistein + NADP+ + H2O
show the reaction diagram
-
-
-
?
additional information
?
-
reduction of soy isoflavones daidzein and genistein under aerobic conditions by use of the recombinant Escherichia coli whole-cell expressing isoflavone reductase
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-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
daidzein + NADPH + O2
2'-hydroxydaidzein + NADP+ + H2O
show the reaction diagram
A0A0U1WKA6
-
-
-
?
genistein + NADPH + O2
2'-hydroxygenistein + NADP+ + H2O
show the reaction diagram
A0A0U1WKA6
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene dgr, recombinant enzyme expression in Escherichia coli strain BL21(DE3), aerobic bioconversion capacity of the recombinant Escherichia coli whole-cell under the optimized conditions, method evaluation and optimization, overview
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
reduction of soy isoflavones by use of Escherichia coli whole-cell biocatalyst expressing isoflavone reductase under aerobic conditions, optimization of the aerobic bioconversion conditions, overview. Addition of chloramphenicol significantly increases the bioconversion capacity of the recombinant Escherichia coli whole-cell, but addition of chloramphenicol does not increase the expression of S-DGR irrespective of whether the concentration of chloramphenicol is high. The maximal concentration of daidzein that the recombinant Escherichia coli whole-cell is able to convert efficiently is 0.8 mmol/l. The average bioconversion rate for daidzein is 85.0% and the yield of 2'-hydroxydaidzein is 0.545 mmol/l