Information on EC 1.14.13.47 - (S)-limonene 3-monooxygenase

for references in articles please use BRENDA:EC1.14.13.47
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The expected taxonomic range for this enzyme is: Mentha x piperita

EC NUMBER
COMMENTARY hide
1.14.13.47
transferred to EC 1.14.14.99
RECOMMENDED NAME
GeneOntology No.
(S)-limonene 3-monooxygenase
PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
Monoterpenoid biosynthesis
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Biosynthesis of secondary metabolites
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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UniProt
Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(S)-limonene + NADPH + H+ + O2
(-)-trans-isopiperitenol + NADP+ + H2O
show the reaction diagram
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?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome P450
a heme-thiolate protein (P-450)
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
the enzyme shows significantly enhanced activity upon RAD52 over-expression and cultivation optimization. (-)-Limonene hydroxylation is 2fold increased by RAD52 overexpression in strains PpPM17/AtCPR, expressing limonene-3-hydroxylase (PM17 isoform) and Arabidopsis thaliana cytochrome P450 reductase (CPR), while cultivation at pH 8 yields in about 55% increased isopiperitenol formation. The two effects are largely additive leading to an about 2.5fold enhanced isopiperitenol production in PpPM17/CPR-RAD52 over the starting strain/condition
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
overexpression in Pichia pastoris. The enzyme shows significantly enhanced activity upon RAD52 over-expression and cultivation optimization. (-)-Limonene hydroxylation is 2fold increased by RAD52 overexpression in strains PpPM17/AtCPR, expressing limonene-3-hydroxylase (PM17 isoform) and Arabidopsis thaliana cytochrome P450 reductase (CPR), while cultivation at pH 8 yields in about 55% increased isopiperitenol formation. The two effects are largely additive leading to an about 2.5fold enhanced isopiperitenol production in PpPM17/CPR-RAD52 over the starting strain/condition