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Information on EC 1.14.13.24 - 3-hydroxybenzoate 6-monooxygenase and Organism(s) Corynebacterium glutamicum and UniProt Accession Q8NLB6

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IUBMB Comments
A flavoprotein (FAD). Acts also on a number of analogues of 3-hydroxybenzoate substituted in the 2, 4, 5 and 6 positions; NADPH can act instead of NADH, but more slowly.
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This record set is specific for:
Corynebacterium glutamicum
UNIPROT: Q8NLB6
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Word Map
The taxonomic range for the selected organisms is: Corynebacterium glutamicum
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
3-hydroxybenzoate 6-hydroxylase, 3hb6h, 3-hydroxybenzoate-6-hydroxylase, 3-hba-6-hydroxylase, ncgl2923, mnx2p, 3-hydroxybenzoate 6-monooxygenase, m-hydroxybenzoate 6-hydroxylase, 3-hydroxybenzoic acid-6-hydroxylase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3-hydroxybenzoate 6-hydroxylase
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3-HBA-6-hydroxylase
-
-
-
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3-hydroxybenzoate 6-hydroxylase
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-
-
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3-hydroxybenzoic acid-6-hydroxylase
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-
-
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m-hydroxybenzoate 6-hydroxylase
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-
-
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oxygenase, 3-hydroxybenzoate 6-mono-
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-
-
-
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
3-hydroxybenzoate + NADH + H+ + O2 = 2,5-dihydroxybenzoate + NAD+ + H2O
show the reaction diagram
catalytic mechanism involving FAD, overview
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
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oxidation
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-
-
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reduction
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-
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SYSTEMATIC NAME
IUBMB Comments
3-hydroxybenzoate,NADH:oxygen oxidoreductase (6-hydroxylating)
A flavoprotein (FAD). Acts also on a number of analogues of 3-hydroxybenzoate substituted in the 2, 4, 5 and 6 positions; NADPH can act instead of NADH, but more slowly.
CAS REGISTRY NUMBER
COMMENTARY hide
51570-26-4
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
3-hydroxybenzoate + NADH + H+ + O2
2,5-dihydroxybenzoate + NAD+ + H2O
show the reaction diagram
-
i.e. gentisate
-
?
3-hydroxybenzoate + NADPH + H+ + O2
2,5-dihydroxybenzoate + NADP+ + H2O
show the reaction diagram
-
i.e. gentisate
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
3-hydroxybenzoate + NADH + H+ + O2
2,5-dihydroxybenzoate + NAD+ + H2O
show the reaction diagram
-
i.e. gentisate
-
?
3-hydroxybenzoate + NADPH + H+ + O2
2,5-dihydroxybenzoate + NADP+ + H2O
show the reaction diagram
-
i.e. gentisate
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
FAD
3fold activation at 0.015 mM. Sequence analysis of FAD/NADPH-binding domain of Ncgl2923, overview
NADH
dependent on, preferred cofactor
NADPH
less active than NADH
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.0534
3-hydroxybenzoate
recombinant enzyme, with NADH, pH 7.5, 25°C
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.92
purified recombinant enzyme, with NADH, pH 7.5, 25°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6 - 8
high activity range
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene ncgl2923
UniProt
Manually annotated by BRENDA team
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
strain ATCC 13032 metabolizes 3-hydroxybenzoate, the 3-hydroxybenzoate 6-hydroxylase is involved in the initial step of 3-hydroxybenzoate catabolismate via gentisate
physiological function
the enzyme catalyzes the NADH-dependent conversion of 3-hydroxybenzoate to gentisate
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
125000
recombinant Ncgl2923, gel filtration
49000
3 * 49000, recombinant Ncgl2923, SDS-PAGE
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trimer
3 * 49000, recombinant Ncgl2923, SDS-PAGE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant N-terminally His-tagged Ncgl2923 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene ncgl2923, sequence determination, analysis, and comparisons, functional overexpression in of N-terminally His-tagged Ncgl2923 in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yang, Y.F.; Zhang, J.J.; Wang, S.H.; Zhou, N.Y.
Purification and characterization of the ncgl2923-encoded 3-hydroxybenzoate 6-hydroxylase from Corynebacterium glutamicum
J. Basic Microbiol.
50
599-604
2010
Corynebacterium glutamicum (Q8NLB6), Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032 (Q8NLB6), Corynebacterium glutamicum ATCC 13032
Manually annotated by BRENDA team