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Enzyme Nomenclature
Information on EC 1.14.13.229 - tert-butyl alcohol monooxygenase
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The enzyme appears in viruses and cellular organisms
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EC NUMBER 
COMMENTARY 
1.14.13.229
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RECOMMENDED NAME
GeneOntology No.
tert-butyl alcohol monooxygenase
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
tert-butyl alcohol + NADPH + H+ + O2 = 2-methylpropane-1,2-diol + NADP+ + H2O
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
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SYSTEMATIC NAME
IUBMB Comments
tert-butyl alcohol,NADPH:oxygen oxidoreductase
The enzyme, characterized from the bacterium Aquincola tertiaricarbonis, is a Rieske nonheme mononuclear iron oxygenase. It can also act, with lower efficiency, on propan-2-ol, converting it to propane-1,2-diol. Depending on the substrate, the enzyme also catalyses EC 1.14.19.48, tert-amyl alcohol desaturase.
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
physiological function
physiological function
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degradation of tert-butyl alcohol leads to formation of isobutene. Both tert-alkyl alcohol degradation and alkene production are strictly oxygen dependent, the relative contribution of the dehydration reaction to total alcohol conversion increases with decreasing oxygen concentrations
physiological function
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degradation of tert-butyl alcohol leads to formation of isobutene. Both tert-alkyl alcohol degradation and alkene production are strictly oxygen dependent, the relative contribution of the dehydration reaction to total alcohol conversion increases with decreasing oxygen concentrations
physiological function
degradation of tert-butyl alcohol leads to formation of isobutene. Both tert-alkyl alcohol degradation and alkene production are strictly oxygen dependent, the relative contribution of the dehydration reaction to total alcohol conversion increases with decreasing oxygen concentrations
physiological function
Mycobacterium austroafricanum grows on methyl tert-butyl ether, on tert-butyl alcohol , and also grows on a broad range of n-alkanes (C2 to C16)
physiological function
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MdpJ knockout mutants are not able to grow on the tertiary alcohols tert-butanol and tert-amyl alcohol, whereas 2-methylpropan-1,2-diol, the putative product of tert-butanol hydroxylation by MdpJ catalysis can still be used as the sole source of energy and carbon. The postulated 2-methylpropan-1,2-diol intermediate 2-hydroxyisobutanoate is metabolized by wild-type and mutant strains at the same rates
physiological function
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degradation of tert-butyl alcohol leads to formation of isobutene. Both tert-alkyl alcohol degradation and alkene production are strictly oxygen dependent, the relative contribution of the dehydration reaction to total alcohol conversion increases with decreasing oxygen concentrations; MdpJ knockout mutants are not able to grow on the tertiary alcohols tert-butanol and tert-amyl alcohol, whereas 2-methylpropan-1,2-diol, the putative product of tert-butanol hydroxylation by MdpJ catalysis can still be used as the sole source of energy and carbon. The postulated 2-methylpropan-1,2-diol intermediate 2-hydroxyisobutanoate is metabolized by wild-type and mutant strains at the same rates
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physiological function
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Mycobacterium austroafricanum grows on methyl tert-butyl ether, on tert-butyl alcohol , and also grows on a broad range of n-alkanes (C2 to C16)
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
tert-butyl alcohol + NADPH + H+ + O2
2-methylpropan-1,2-diol + NADP+ + 2 H2O
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?
tert-butyl alcohol + NADPH + H+ + O2
2-methylpropan-1,2-diol + NADP+ + 2 H2O
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product is further metabolized to 2-hydroxyisobutanoate
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?
tert-butyl alcohol + NADPH + H+ + O2
2-methylpropan-1,2-diol + NADP+ + 2 H2O
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?
tert-butyl alcohol + NADPH + H+ + O2
2-methylpropan-1,2-diol + NADP+ + 2 H2O
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product is further metabolized to 2-hydroxyisobutanoate
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?
tert-butyl alcohol + NADPH + H+ + O2
2-methylpropane-1,2-diol + NADP+ + H2O
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?
tert-butyl alcohol + NADPH + H+ + O2
2-methylpropane-1,2-diol + NADP+ + H2O
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?
additional information
?
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the enzyme also catalyses the reaction of EC 1.14.19.48, tert-amyl alcohol desaturase
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additional information
?
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the enzyme also catalyses the reaction of EC 1.14.19.48, tert-amyl alcohol desaturase
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additional information
?
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the enzyme also catalyses the reaction of EC 1.14.19.48, tert-amyl alcohol desaturase
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additional information
?
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the enzyme also catalyses the reaction of EC 1.14.19.48, tert-amyl alcohol desaturase
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-
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additional information
?
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the enzyme also catalyses the reaction of EC 1.14.19.48, tert-amyl alcohol desaturase
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
[2Fe-2S]-center
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sequence contains conserved amino acid motif -R-x12-CxHRxxxLxxG-x8-CxYHR-x6-G- for the Rieske [2Fe-2S] binding domain
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Iron
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sequence contains conserved amino acid motif (-D/E)xxxDxxHxxxxH- for a mononuclear iron binding domain
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SUBUNITS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
expression is induced after growth on n-propane, n-hexane, n-hexadecane and on tert-butyl alcohol
expression is induced after growth on n-propane, n-hexane, n-hexadecane and on tert-butyl alcohol
expression is induced after growth on n-propane, n-hexane, n-hexadecane and on tert-butyl alcohol
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LINKS TO OTHER DATABASES (specific for EC-Number 1.14.13.229)
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