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IUBMB Comments The enzyme, characterized from the bacterium Rhodococcus jostii RHA1, is a single-component hydroxylase. The enzyme has no activity with NADPH. cf . EC 1.14.13.219 , resorcinol 4-hydroxylase (NADPH), and EC 1.14.14.27 , resorcinol 4-hydroxylase (FADH2
Synonyms tsdB ,
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resorcinol + NADH + H+ + O2 = hydroxyquinol + NAD+ + H2O
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MetaCyc
gamma-resorcylate degradation II
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resorcinol,NADH:oxygen oxidoreductase (4-hydroxylating)
The enzyme, characterized from the bacterium Rhodococcus jostii RHA1, is a single-component hydroxylase. The enzyme has no activity with NADPH. cf. EC 1.14.13.219, resorcinol 4-hydroxylase (NADPH), and EC 1.14.14.27, resorcinol 4-hydroxylase (FADH2).
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resorcinol + NADH + H+ + O2
hydroxyquinol + NAD+ + H2O
resorcinol + NADH + H+ + O2
hydroxyquinol + NAD+ + H2O
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Substrates: the enzyme gamma-resorcylate catabolic-pathway
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resorcinol + NADH + H+ + O2
hydroxyquinol + NAD+ + H2O
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Substrates: resorcinol i.e. benzene-1,3-diol. The enzyme has no activity with NADPH
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resorcinol + NADH + H+ + O2
hydroxyquinol + NAD+ + H2O
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Substrates: the enzyme gamma-resorcylate catabolic-pathway
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NADH
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the enzyme has no activity with NADPH
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Highest Expressing Human Cell Lines
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Cell Line Links
Gene Links
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metabolism
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the enzyme gamma-resorcylate catabolic-pathway
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TSDB_RHOJR
Rhodococcus jostii (strain RHA1)
530
0
57772
Swiss-Prot
other Location (Reliability: 3 )
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59000
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1 * 59000, SDS-PAGE
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monomer
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1 * 59000, SDS-PAGE
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recombinant His-tagged protein
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expression in Escherichia coli BL21
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transcription of tsdB is activated when cells are grown on gamma-resorcylate
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Kasai, D.; Araki, N.; Motoi, K.; Yoshikawa, S.; Iino, T.; Imai, S.; Masai, E.; Fukuda, M.
gamma-Resorcylate catabolic-pathway genes in the soil actinomycete Rhodococcus jostii RHA1
Appl. Environ. Microbiol.
81
7656-7665
2015
Rhodococcus jostii
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