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Information on EC 1.14.13.122 - chlorophyllide-a oxygenase and Organism(s) Chlamydomonas reinhardtii and UniProt Accession Q9ZWM5

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IUBMB Comments
Chlorophyll b is required for the assembly of stable light-harvesting complexes (LHCs) in the chloroplast of green algae, cyanobacteria and plants [2,3]. Contains a mononuclear iron centre . The enzyme catalyses two successive hydroxylations at the 7-methyl group of chlorophyllide a. The second step yields the aldehyde hydrate, which loses H2O spontaneously to form chlorophyllide b . Chlorophyll a and protochlorophyllide a are not substrates .
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Chlamydomonas reinhardtii
UNIPROT: Q9ZWM5
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Word Map
The taxonomic range for the selected organisms is: Chlamydomonas reinhardtii
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Reaction Schemes
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Synonyms
chlorophyllide a oxygenase, chlorophyll a oxygenase, oscao1, zmcao1, atptc52, pchlide a oxygenase, chlorophyllide a oxygenase 1, chlorophyll a oxygenase/chlorophyll b synthase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
chlorophyll a oxygenase
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chlorophyllide a oxygenase
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-
-
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
chlorophyllide a + O2 + NADPH + H+ = 71-hydroxychlorophyllide a + H2O + NADP+
show the reaction diagram
reaction mechanism
chlorophyllide a + 2 O2 + 2 NADPH + 2 H+ = chlorophyllide b + 3 H2O + 2 NADP+
show the reaction diagram
reaction mechanism
chlorophyllide a + O2 + NADPH + H+ = 71-hydroxychlorophyllide a + H2O + NADP+
show the reaction diagram
reaction mechanism, the catalytic site harbors a stable radical formed by Tyr422 which is involved in the catalytic reaction
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chlorophyllide a + 2 O2 + 2 NADPH + 2 H+ = chlorophyllide b + 3 H2O + 2 NADP+
show the reaction diagram
reaction mechanism, the catalytic site harbors a stable radical formed by Tyr422 which is involved in the catalytic reaction
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SYSTEMATIC NAME
IUBMB Comments
chlorophyllide-a:oxygen 7-oxidoreductase
Chlorophyll b is required for the assembly of stable light-harvesting complexes (LHCs) in the chloroplast of green algae, cyanobacteria and plants [2,3]. Contains a mononuclear iron centre [3]. The enzyme catalyses two successive hydroxylations at the 7-methyl group of chlorophyllide a. The second step yields the aldehyde hydrate, which loses H2O spontaneously to form chlorophyllide b [2]. Chlorophyll a and protochlorophyllide a are not substrates [2].
CAS REGISTRY NUMBER
COMMENTARY hide
216503-73-0
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
7-hydroxychlorophyllide a + O2 + NADPH
chlorophyllide b + H2O + NADP+
show the reaction diagram
-
-
-
?
chlorophyllide a + O2 + NADPH + H+
7-hydroxychlorophyllide a + H2O + NADP+
show the reaction diagram
-
-
-
?
7-hydroxychlorophyllide a + O2 + NADPH
chlorophyllide b + H2O + NADP+
show the reaction diagram
-
-
-
-
?
chlorophyllide a + O2 + NADPH + H+
7-hydroxychlorophyllide a + H2O + NADP+
show the reaction diagram
-
-
-
-
?
additional information
?
-
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
7-hydroxychlorophyllide a + O2 + NADPH
chlorophyllide b + H2O + NADP+
show the reaction diagram
-
-
-
?
chlorophyllide a + O2 + NADPH + H+
7-hydroxychlorophyllide a + H2O + NADP+
show the reaction diagram
-
-
-
?
7-hydroxychlorophyllide a + O2 + NADPH
chlorophyllide b + H2O + NADP+
show the reaction diagram
-
-
-
-
?
chlorophyllide a + O2 + NADPH + H+
7-hydroxychlorophyllide a + H2O + NADP+
show the reaction diagram
-
-
-
-
?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Fe2+
the enzyme might contain a [2Fe-2S] Rieske cluster
Fe2+
-
the enzyme contains a [2Fe-2S] Rieske cluster and a mononuclear non-hem iron-binding site
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
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envelope inner membrane, in light and dark grown cells
Manually annotated by BRENDA team
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only in light grown cells
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CAO_CHLRE
645
0
72106
Swiss-Prot
Mitochondrion (Reliability: 3)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
construction of six chlorophyll-b less mutants by insertional mutagenesis, analysis of DNA rearrangements, overview
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
construction of a genomic library, DNA and amino acid sequence determination
expression of GFP-tagged enzyme in Chlamydomonas reinhardtii cells, phylogenetic tree
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Eggink, L.L.; LoBrutto, R.; Brune, D.C.; Brusslan, J.; Yamasato, A.; Tanaka, A.; Hoober, J.K.
Synthesis of chlorophyll b: localization of chlorophyllide a oxygenase and discovery of a stable radical in the catalytic subunit
BMC Plant Biol.
4
5
2004
Arabidopsis thaliana, Chlamydomonas reinhardtii, Chlamydomonas reinhardtii cw15
Manually annotated by BRENDA team
Tanaka, A.; Ito, H.; Tanaka, R.; Tanaka, N.K.; Yoshida, K.; Okada, K.
Chlorophyll a oxygenase (CAO) is involved in chlorophyll b formation from chlorophyll a
Proc. Natl. Acad. Sci. USA
95
12719-12723
1998
Chlamydomonas reinhardtii (Q9ZWM5), Chlamydomonas reinhardtii 5D (Q9ZWM5)
Manually annotated by BRENDA team