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Information on EC 1.14.12.25 - p-cumate 2,3-dioxygenase and Organism(s) Pseudomonas putida and UniProt Accession Q51974

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IUBMB Comments
The enzyme, characterized from several Pseudomonas strains, is involved in the degradation of p-cymene and p-cumate. It comprises four components: a ferredoxin, a ferredoxin reductase, and two subunits of a catalytic component. The enzyme can also act on indole, transforming it to the water-insoluble blue dye indigo.
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This record set is specific for:
Pseudomonas putida
UNIPROT: Q51974 not found.
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The taxonomic range for the selected organisms is: Pseudomonas putida
The enzyme appears in selected viruses and cellular organisms
Synonyms
p-cumate dioxygenase, cmtab, cmtad, p-cumate 2,3-dioxygenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
CmtAa
Q51973; Q51974; Q51975; Q51978
-
CmtAb
Q51973; Q51974; Q51975; Q51978
-
CmtAc
Q51973; Q51974; Q51975; Q51978
-
CmtAd
Q51973; Q51974; Q51975; Q51978
-
SYSTEMATIC NAME
IUBMB Comments
4-isopropylbenzoate:oxygen 2,3-oxidoreductase
The enzyme, characterized from several Pseudomonas strains, is involved in the degradation of p-cymene and p-cumate. It comprises four components: a ferredoxin, a ferredoxin reductase, and two subunits of a catalytic component. The enzyme can also act on indole, transforming it to the water-insoluble blue dye indigo.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
indole-2-carboxylate + NADH + H+ + O2
2,3-dihydroxy-2,3-dihydro-1H-indole-2-carboxylic acid + NAD+
show the reaction diagram
-
product rearranges spontaneously forming indigo, indirubin, and isatin
-
-
?
indole-3-carboxylate + NADH + H+ + O2
2,3-dihydroxy-2,3-dihydro-1H-indole-3-carboxylic acid + NAD+
show the reaction diagram
-
-
-
-
?
m-toluate + NADH + H+ + O2
1,6-dihydroxy-5-methylcyclohexa-2,4-diene-1-carboxylic acid + NAD+
show the reaction diagram
-
-
-
-
?
p-cumate + NADH + H+ + O2
2,3-dihydroxy-2,3-dihydro-p-cumate + NAD+
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
p-cumate + NADH + H+ + O2
2,3-dihydroxy-2,3-dihydro-p-cumate + NAD+
show the reaction diagram
-
-
the substrate for the ring cleavage of 2,3-dihydroxy-p-cumate is formed from p-cumate in two reactions via a dihydrodiol intermediate (2,3-dihydroxy-4-isopropylcyclohexa-4,6-dienoate). Both hydroxyl atoms of the dihydrodiol are derived from the same molecule of 02
-
?
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
-
a mutant of Pseudomonas putida, which is unable to grow with p-cumate, accumulates 2,3-dihydroxy-4-isoproplycyclohexa-4,6-dienoate. Both hydroxyl atoms of the dihydrodiol are derived from the same molecule of 02
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
CMTAB_PSEPU
434
0
48968
Swiss-Prot
-
SUBUNIT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
-
enrichment of mutants defective in the utilization of p-cymene as sole carbon source by incubation of cells in minimal growth media containing p-cymene, p-chlorotoluene, p-bromotoluene, alpha-chloro-p-xylene, or p-iodobenzoate. Several classes of mutants can be isolated, p-cymene hydroxylase and p-cumate 2,3-dioxygenase are likely to be multicomponent enzymes
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
DeFrank, J.; Ribbons, D.
p-Cymene pathway in Pseudomonas putida: initial reactions
J. Bacteriol.
129
1356-1364
1977
Pseudomonas putida
Manually annotated by BRENDA team
Wigmore, G.; Ribbons, D.
p-Cymene pathway in Pseudomonas putida: selective enrichment of defective mutants by using halogenated substrate analogs
J. Bacteriol.
143
816-824
1980
Pseudomonas putida
Manually annotated by BRENDA team
Eaton, R.; Chapman, P.
Formation of indigo and related compounds from indolecarboxylic acids by aromatic acid-degrading bacteria: Chromogenic reactions for cloning genes encoding dioxygenases that act on aromatic acids
J. Bacteriol.
177
6983-6988
1995
Pseudomonas putida
Manually annotated by BRENDA team
Eaton, R.W.
p-Cumate catabolic pathway in Pseudomonas putida Fl: cloning and characterization of DNA carrying the cmt operon
J. Bacteriol.
178
1351-1362
1996
Pseudomonas putida (Q51973 and Q51974 and Q51975 and Q51978), Pseudomonas putida, Pseudomonas putida DSM 6899 (Q51973 and Q51974 and Q51975 and Q51978)
Manually annotated by BRENDA team