Any feedback?
No. of entries
Information on EC 1.14.12.24 - 2,4-dinitrotoluene dioxygenase
for references in articles please use BRENDA:EC1.14.12.24
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree 1 Oxidoreductases
1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen
1.14.12 With NADH or NADPH as one donor, and incorporation of two atoms of oxygen into the other donor
1.14.12.24 2,4-dinitrotoluene dioxygenase
IUBMB Comments
This enzyme, characterized from the bacterium Burkholderia sp. strain DNT, is a member of the naphthalene family of bacterial Rieske non-heme iron dioxygenases. It comprises a multicomponent system, containing a Rieske [2Fe-2S] ferredoxin, an NADH-dependent flavoprotein reductase (EC 1.18.1.3, ferredoxin—NAD+ reductase), and an α3β3 oxygenase. The enzyme forms a cis-dihydroxylated product that spontaneously rearranges to form a catechol with accompanying release of nitrite. It does not act on nitrobenzene. cf. EC 1.14.12.23, nitroarene dioxygenase.
The enzyme appears in viruses and cellular organisms
- 1.14.12.24
- burkholderia
- 4-methyl-5-nitrocatechol
- cepacia
- monooxygenase
- naphthalene
- 2-nitrotoluene
-
fission
-
dinitrotoluenes
- sphingomonas
-
nitroaromatic
-
extradiol
- nahac
- vitreoscilla
-
regiospecificity
-
enantiomeric
-
priority
showSynonyms
2,4-dnt dioxygenase, dnt dioxygenase, dntac, 2,4-dntdo, 2,4-dinitrotoluene dioxygenase, more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
2,4-dinitrotoluene dioxygenase
2,4-DNT dioxygenase
2,4-DNTDO
DDO
DNT dioxygenase
dntA
DntAc
DNTDO
dntA
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
2,4-dinitrotoluene + NADH + O2 = 4-methyl-5-nitrocatechol + nitrite + NAD+
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
PATHWAY SOURCE
PATHWAYS
MetaCyc
2,4-dinitrotoluene degradation
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
2,4-dinitrotoluene,NADH:oxygen oxidoreductase (4,5-hydroxylating, nitrite-releasing)
This enzyme, characterized from the bacterium Burkholderia sp. strain DNT, is a member of the naphthalene family of bacterial Rieske non-heme iron dioxygenases. It comprises a multicomponent system, containing a Rieske [2Fe-2S] ferredoxin, an NADH-dependent flavoprotein reductase (EC 1.18.1.3, ferredoxin---NAD+ reductase), and an alpha3beta3 oxygenase. The enzyme forms a cis-dihydroxylated product that spontaneously rearranges to form a catechol with accompanying release of nitrite. It does not act on nitrobenzene. cf. EC 1.14.12.23, nitroarene dioxygenase.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2-amino-5-nitrotoluene + NADH + H+ + O2
2-amino-5-nitrobenzyl alcohol + aminonitrocresol + NAD+ + H2O
2-amino-5-nitrotoluene + NADH + O2
2-amino-5-nitrobenzylalcohol + aminonitrocresol + nitrite + NAD+
2-amino-6-nitrotoluene + NADH + H+ + O2
2-amino-4-nitro-3-cresol + 3-amino-5-nitro-4-cresol + NAD+ + H2O
2-amino-6-nitrotoluene + NADH + O2
2-amino-4-nitro-m-cresol + 3-amino-5-nitro-p-cresol + nitrite + NAD+
3-amino-4-nitrotoluene + NADH + H+ + O2
3-amino-4-nitrobenzyl alcohol + aminonitrocresol + NAD+ + H2O
Substrates: -
Products: -
Products: -
?
3-amino-4-nitrotoluene + NADH + O2
3-amino-4-nitrobenzylalcohol + aminonitrocresol + nitrite + NAD+
4-amino-2-nitrotoluene + NADH + H+ + O2
aminonitrocresol + NAD+ + H2O
Substrates: -
Products: -
Products: -
?
4-amino-2-nitrotoluene + NADH + O2
aminonitrocresol + nitrite + NAD+
Substrates: 13% yield
Products: -
Products: -
?
4-amino-3-nitrotoluene + NADH + O2
4-amino-3-nitrobenzylalcohol + aminonitrocresol + ?
Substrates: -
Products: -
Products: -
?
4-amino-3-nitrotoluene + NADH + O2
4-amino-3-nitrobenzylalcohol + aminonitrocresol + nitrite + NAD+
Substrates: 19% yield
Products: -
Products: -
?
4-methyl-5-nitrocatechol + nitrite + NAD+
2,4-dinitrotoluene + NADH + O2
-
Substrates: -
Products: -
Products: -
r
4-nitrotoluene + NADH + O2
4-methylcatechol + nitrite + NAD+
Substrates: -
Products: -
Products: -
?
5-amino-2-nitrotoluene + NADH + H+ + O2
2-amino-5-nitro-o-cresol + NAD+ + H2O
Substrates: -
Products: -
Products: -
?
5-amino-2-nitrotoluene + NADH + O2
2-amino-5-nitro-o-cresol + nitrite + NAD+
Substrates: -
Products: 24% yield
Products: 24% yield
?
acenaphthene + NADH + H+ + O2
1-acenaphthenol + NAD+ + ?
Substrates: -
Products: -
Products: -
?
indan + NADH + H+ + O2
1-indanol + 1-indanone + indene + NAD+
Substrates: -
Products: -
Products: -
?
indene + NADH + H+ + O2
1-indenol + 1,2-dihydroxyindan + NAD+
Substrates: -
Products: -
Products: -
?
indole + NADH + H+ + O2
indigo + NAD+ + ?
Substrates: -
Products: -
Products: -
?
indoline + NADH + H+ + O2
indole + NAD+ + ?
Substrates: -
Products: -
Products: -
?
naphthalene + NADH + H+ + O2
(+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+
Substrates: -
Products: -
Products: -
?
naphthalene + NADH + O2
(1R,3S)-cis-1,2-dihydro-1,2-dihydroxynaphthalene + NAD+ + H2O
-
Substrates: -
Products: -
Products: -
?
o-cresol + NADH + H+ + O2
methylhydroquinone + 2-hydroxybenzyl alcohol + nitrite + NAD+
Substrates: -
Products: mutant V350F produces methylhydroquinone at 1.52 nmol/min/mg of protein and 2-hydroxybenzyl alcohol at 0.74 nmol/min/mg of protein. Mutant V350M produces methylhydroquinone at 1.97 nmol/min/mg of protein and 2-hydroxybenzyl alcohol at 0.11 nmol/min/mg of protein
Products: mutant V350F produces methylhydroquinone at 1.52 nmol/min/mg of protein and 2-hydroxybenzyl alcohol at 0.74 nmol/min/mg of protein. Mutant V350M produces methylhydroquinone at 1.97 nmol/min/mg of protein and 2-hydroxybenzyl alcohol at 0.11 nmol/min/mg of protein
?
o-methoxyphenol + NADH + H+ + O2
methoxyhydroquinone + nitrite + NAD+
Substrates: -
Products: mutant V350F produces methoxyhydroquinone at 2.5 nmol/min/mg of protein. Mutant V350M produces methoxyhydroquinone at 2.4 mol/min/mg of protein
Products: mutant V350F produces methoxyhydroquinone at 2.5 nmol/min/mg of protein. Mutant V350M produces methoxyhydroquinone at 2.4 mol/min/mg of protein
?
o-nitrophenol + NADH + H+ + O2
nitrohydroquinone + 3-nitrocatechol + nitrite + NAD+
Substrates: -
Products: product of wild-type. Mutant V350F produces both nitrohydroquinone at a rate of 0.75 nmol/min/mg of protein and 3-nitrocatechol at a rate of 0.069 nmol/min/mg of protein from o-nitrophenol. Mutant V350M produces both nitrohydroquinone at 0.33 nmol/min/mg of protein and 3-nitrocatechol at 0.089 nmol/min/mg of protein
Products: product of wild-type. Mutant V350F produces both nitrohydroquinone at a rate of 0.75 nmol/min/mg of protein and 3-nitrocatechol at a rate of 0.069 nmol/min/mg of protein from o-nitrophenol. Mutant V350M produces both nitrohydroquinone at 0.33 nmol/min/mg of protein and 3-nitrocatechol at 0.089 nmol/min/mg of protein
?
phenetole + NADH + H+ + O2
phenol + NAD+ + ?
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + H+ + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + H+ + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + H+ + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + H+ + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + H+ + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + H+ + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + H+ + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + H+ + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
Substrates: highest activity
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
Substrates: highest activity
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
Substrates: -
Products: formation of 9.54 nmol 3-methylcatechol per mg protein plus 1.06 nmol 2-nitrobenzyl alcohol
Products: formation of 9.54 nmol 3-methylcatechol per mg protein plus 1.06 nmol 2-nitrobenzyl alcohol
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
Substrates: best substrate
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
Substrates: -
Products: formation of 9.54 nmol 3-methylcatechol per mg protein plus 1.06 nmol 2-nitrobenzyl alcohol
Products: formation of 9.54 nmol 3-methylcatechol per mg protein plus 1.06 nmol 2-nitrobenzyl alcohol
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
Substrates: best substrate
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
Substrates: -
Products: -
Products: -
r
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
Substrates: -
Products: -
Products: -
r
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
Substrates: -
Products: -
Products: -
?
2,6-dinitrotoluene + NADH + O2
3-methyl-4-nitrocatechol + nitrite + NAD+
Substrates: -
Products: -
Products: -
?
2,6-dinitrotoluene + NADH + O2
3-methyl-4-nitrocatechol + nitrite + NAD+
Substrates: -
Products: -
Products: -
?
2-amino-3-nitrotoluene + NADH + H+ + O2
2-amino-3-nitrobenzylalcohol + NAD+ + H2O
Substrates: -
Products: -
Products: -
?
2-amino-3-nitrotoluene + NADH + H+ + O2
2-amino-3-nitrobenzylalcohol + NAD+ + H2O
Substrates: -
Products: -
Products: -
?
2-amino-3-nitrotoluene + NADH + O2
2-amino-3-nitrobenzylalcohol + nitrite + NAD+
Substrates: -
Products: 27% yield
Products: 27% yield
?
2-amino-3-nitrotoluene + NADH + O2
2-amino-3-nitrobenzylalcohol + nitrite + NAD+
Substrates: -
Products: 27% yield
Products: 27% yield
?
2-amino-5-nitrotoluene + NADH + H+ + O2
2-amino-5-nitrobenzyl alcohol + aminonitrocresol + NAD+ + H2O
Substrates: -
Products: -
Products: -
?
2-amino-5-nitrotoluene + NADH + H+ + O2
2-amino-5-nitrobenzyl alcohol + aminonitrocresol + NAD+ + H2O
Substrates: -
Products: -
Products: -
?
2-amino-5-nitrotoluene + NADH + O2
2-amino-5-nitrobenzylalcohol + aminonitrocresol + nitrite + NAD+
Substrates: -
Products: 34.4% yield
Products: 34.4% yield
?
2-amino-5-nitrotoluene + NADH + O2
2-amino-5-nitrobenzylalcohol + aminonitrocresol + nitrite + NAD+
Substrates: -
Products: 34.4% yield
Products: 34.4% yield
?
2-amino-6-nitrotoluene + NADH + H+ + O2
2-amino-4-nitro-3-cresol + 3-amino-5-nitro-4-cresol + NAD+ + H2O
Substrates: -
Products: -
Products: -
?
2-amino-6-nitrotoluene + NADH + H+ + O2
2-amino-4-nitro-3-cresol + 3-amino-5-nitro-4-cresol + NAD+ + H2O
Substrates: -
Products: -
Products: -
?
2-amino-6-nitrotoluene + NADH + O2
2-amino-4-nitro-m-cresol + 3-amino-5-nitro-p-cresol + nitrite + NAD+
Substrates: -
Products: 20% yield
Products: 20% yield
?
2-amino-6-nitrotoluene + NADH + O2
2-amino-4-nitro-m-cresol + 3-amino-5-nitro-p-cresol + nitrite + NAD+
Substrates: -
Products: 20% yield
Products: 20% yield
?
2-methoxyphenol + NADH + O2
methoxyhydroquinone + NAD+ + H2O
-
Substrates: -
Products: -
Products: -
?
2-methoxyphenol + NADH + O2
methoxyhydroquinone + NAD+ + H2O
-
Substrates: -
Products: -
Products: -
?
2-nitrophenol + NADH + O2
nitrohydroquinone + 3-nitrocatechol + NAD+ + H2O
-
Substrates: -
Products: -
Products: -
?
2-nitrophenol + NADH + O2
nitrohydroquinone + 3-nitrocatechol + NAD+ + H2O
-
Substrates: -
Products: -
Products: -
?
2-nitrotoluene + NADH + H+ + O2
2-nitrobenzyl alcohol + NAD+ + ?
Substrates: -
Products: -
Products: -
?
2-nitrotoluene + NADH + H+ + O2
2-nitrobenzyl alcohol + NAD+ + ?
Substrates: -
Products: -
Products: -
?
2-nitrotoluene + NADH + H+ + O2
2-nitrobenzyl alcohol + NAD+ + ?
Substrates: -
Products: -
Products: -
?
2-nitrotoluene + NADH + H+ + O2
2-nitrobenzyl alcohol + NAD+ + ?
Substrates: -
Products: -
Products: -
?
2-nitrotoluene + NADH + H+ + O2
2-nitrobenzyl alcohol + NAD+ + H2O
Substrates: -
Products: -
Products: -
?
2-nitrotoluene + NADH + H+ + O2
2-nitrobenzyl alcohol + NAD+ + H2O
Substrates: -
Products: -
Products: -
?
3-amino-4-nitrotoluene + NADH + O2
3-amino-4-nitrobenzylalcohol + aminonitrocresol + nitrite + NAD+
Substrates: -
Products: 9% yield
Products: 9% yield
?
3-amino-4-nitrotoluene + NADH + O2
3-amino-4-nitrobenzylalcohol + aminonitrocresol + nitrite + NAD+
Substrates: -
Products: 9% yield
Products: 9% yield
?
3-nitrophenol + NADH + O2
4-nitrocatechol + NAD+ + H2O
-
Substrates: -
Products: -
Products: -
?
3-nitrophenol + NADH + O2
4-nitrocatechol + NAD+ + H2O
-
Substrates: -
Products: -
Products: -
?
3-nitrotoluene + NADH + H+ + O2
3-nitrobenzyl alcohol + NAD+ + ?
Substrates: -
Products: -
Products: -
?
3-nitrotoluene + NADH + H+ + O2
3-nitrobenzyl alcohol + NAD+ + ?
Substrates: -
Products: -
Products: -
?
3-nitrotoluene + NADH + O2
4-methylcatechol + nitrite + NAD+
-
Substrates: -
Products: formation of 0.06 nmol 4-methylcatechol per mg protein plus 0.03 nmol 3-nitrobenzyl alcohol
Products: formation of 0.06 nmol 4-methylcatechol per mg protein plus 0.03 nmol 3-nitrobenzyl alcohol
?
3-nitrotoluene + NADH + O2
4-methylcatechol + nitrite + NAD+
-
Substrates: -
Products: formation of 0.06 nmol 4-methylcatechol per mg protein plus 0.03 nmol 3-nitrobenzyl alcohol
Products: formation of 0.06 nmol 4-methylcatechol per mg protein plus 0.03 nmol 3-nitrobenzyl alcohol
?
4-nitrotoluene + NADH + H+ + O2
4-nitrobenzyl alcohol + NAD+ + ?
Substrates: -
Products: -
Products: -
?
4-nitrotoluene + NADH + H+ + O2
4-nitrobenzyl alcohol + NAD+ + ?
Substrates: -
Products: -
Products: -
?
indan + NADH + H+ + O2
1-indanol + ? + NAD+
Substrates: -
Products: no formation of 1,2-dihydroxyindan
Products: no formation of 1,2-dihydroxyindan
?
indan + NADH + H+ + O2
1-indanol + ? + NAD+
Substrates: -
Products: no formation of 1,2-dihydroxyindan
Products: no formation of 1,2-dihydroxyindan
?
indoline + NADH + H+ + O2
indigo + ? + NAD+
Substrates: -
Products: -
Products: -
?
indoline + NADH + H+ + O2
indigo + ? + NAD+
Substrates: -
Products: -
Products: -
?
m-cresol + NADH + H+ + O2
methylhydroquinone + nitrite + NAD+
Substrates: -
Products: no substrate for wild-type. Mutant V350F produces methylhydroquinone at 0.43 nmol/min/mg of protein
Products: no substrate for wild-type. Mutant V350F produces methylhydroquinone at 0.43 nmol/min/mg of protein
?
m-cresol + NADH + H+ + O2
methylhydroquinone + nitrite + NAD+
Substrates: -
Products: no substrate for wild-type. Mutant V350F produces methylhydroquinone at 0.43 nmol/min/mg of protein
Products: no substrate for wild-type. Mutant V350F produces methylhydroquinone at 0.43 nmol/min/mg of protein
?
m-methoxyphenol + NADH + H+ + O2
methoxyhydroquinone + nitrite + NAD+
Substrates: -
Products: no substrate for wild-type. Mutant V350F produces methoxyhydroquinone at 0.55 nmol/min/mg of protein
Products: no substrate for wild-type. Mutant V350F produces methoxyhydroquinone at 0.55 nmol/min/mg of protein
?
m-methoxyphenol + NADH + H+ + O2
methoxyhydroquinone + nitrite + NAD+
Substrates: -
Products: no substrate for wild-type. Mutant V350F produces methoxyhydroquinone at 0.55 nmol/min/mg of protein
Products: no substrate for wild-type. Mutant V350F produces methoxyhydroquinone at 0.55 nmol/min/mg of protein
?
m-nitrophenol + NADH + H+ + O2
4-nitrocatechol + nitrite + NAD+
Substrates: -
Products: no substrate for wild-type. Mutant V350F produces 4-nitrocatechol at 0.29 nmol/min/mg of protein
Products: no substrate for wild-type. Mutant V350F produces 4-nitrocatechol at 0.29 nmol/min/mg of protein
?
m-nitrophenol + NADH + H+ + O2
4-nitrocatechol + nitrite + NAD+
Substrates: -
Products: no substrate for wild-type. Mutant V350F produces 4-nitrocatechol at 0.29 nmol/min/mg of protein
Products: no substrate for wild-type. Mutant V350F produces 4-nitrocatechol at 0.29 nmol/min/mg of protein
?
naphthalene + NADH + H+ + O2
(+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+ + ?
Substrates: -
Products: -
Products: -
?
naphthalene + NADH + H+ + O2
(+)-cis-(1R,2S)-dihydroxy-1,2-dihydronaphthalene + NAD+ + ?
Substrates: -
Products: -
Products: -
?
naphthalene + NADH + H+ + O2
(1R,2S)-cis-1,2-dihydroxy-1,2-dihydronaphthalene + NAD+
Substrates: -
Products: -
Products: -
?
naphthalene + NADH + H+ + O2
(1R,2S)-cis-1,2-dihydroxy-1,2-dihydronaphthalene + NAD+
Substrates: -
Products: mutants V350F and V350M exhibit 10fold enhanced activity towards naphthalene
Products: mutants V350F and V350M exhibit 10fold enhanced activity towards naphthalene
?
naphthalene + NADH + H+ + O2
(1R,2S)-cis-1,2-dihydroxy-1,2-dihydronaphthalene + NAD+
Substrates: -
Products: mutants V350F and V350M exhibit 10fold enhanced activity towards naphthalene
Products: mutants V350F and V350M exhibit 10fold enhanced activity towards naphthalene
?
naphthalene + NADH + H+ + O2
(1R,2S)-cis-1,2-dihydroxy-1,2-dihydronaphthalene + NAD+
Substrates: -
Products: -
Products: -
?
naphthalene + O2
cis-1,2-dihydroxy-1,2-dihydronaphthalene
-
Substrates: -
Products: formation of 0.83 nmol 3-methylcatechol per mg protein
Products: formation of 0.83 nmol 3-methylcatechol per mg protein
?
naphthalene + O2
cis-1,2-dihydroxy-1,2-dihydronaphthalene
Substrates: -
Products: product is formed in 92% enantiomeric excess
Products: product is formed in 92% enantiomeric excess
?
naphthalene + O2
cis-1,2-dihydroxy-1,2-dihydronaphthalene
-
Substrates: -
Products: formation of 0.83 nmol 3-methylcatechol per mg protein
Products: formation of 0.83 nmol 3-methylcatechol per mg protein
?
naphthalene + O2
cis-1,2-dihydroxy-1,2-dihydronaphthalene
Substrates: -
Products: product is formed in 92% enantiomeric excess
Products: product is formed in 92% enantiomeric excess
?
additional information
?
-
-
Substrates: no activity with 2-cresol, 3-methoxyphenol
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with 2-cresol, 3-methoxyphenol
Products: -
Products: -
?
additional information
?
-
Substrates: no activity with 2,3-dinitrotoluene and 2,5-dinitrotoluene
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with 2,3-dinitrotoluene and 2,5-dinitrotoluene
Products: -
Products: -
?
additional information
?
-
-
Substrates: 2-amino-4-nitrotoluene is not a substrate
Products: -
Products: -
?
additional information
?
-
Substrates: 2-amino-4-nitrotoluene is not a substrate
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with anisole, carbazole, and nitrobenzene
Products: -
Products: -
?
additional information
?
-
Substrates: no activity with anisole, carbazole, and nitrobenzene
Products: -
Products: -
?
additional information
?
-
-
Substrates: no substrate: 2-amino-4-nitrotoluene
Products: -
Products: -
?
additional information
?
-
Substrates: no substrate: 2-amino-4-nitrotoluene
Products: -
Products: -
?
additional information
?
-
-
Substrates: no substrate: 2-nitrotoluene, 3-nitrotoluene
Products: -
Products: -
?
additional information
?
-
-
Substrates: 2-amino-4-nitrotoluene is not a substrate
Products: -
Products: -
?
additional information
?
-
Substrates: 2-amino-4-nitrotoluene is not a substrate
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with anisole, carbazole, and nitrobenzene
Products: -
Products: -
?
additional information
?
-
Substrates: no activity with anisole, carbazole, and nitrobenzene
Products: -
Products: -
?
additional information
?
-
-
Substrates: no substrate: 2-nitrotoluene, 3-nitrotoluene
Products: -
Products: -
?
additional information
?
-
-
Substrates: no substrate: 2-amino-4-nitrotoluene
Products: -
Products: -
?
additional information
?
-
Substrates: no substrate: 2-amino-4-nitrotoluene
Products: -
Products: -
?
additional information
?
-
Substrates: no activity with 2,3-dinitrotoluene and 2,5-dinitrotoluene
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with 3-nitrotoluene, 4-nitrotoluene, catechol, 3-nitrocatechol, 3-methylcatechol, and 4-methylcatechol
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with 3-nitrotoluene, 4-nitrotoluene, catechol, 3-nitrocatechol, 3-methylcatechol, and 4-methylcatechol
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2,4-dinitrotoluene + NADH + H+ + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + H+ + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + H+ + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + H+ + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + H+ + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + H+ + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
Substrates: highest activity
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
Substrates: highest activity
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
Substrates: -
Products: -
Products: -
?
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
Substrates: -
Products: -
Products: -
r
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
Substrates: -
Products: -
Products: -
r
2,4-dinitrotoluene + NADH + O2
4-methyl-5-nitrocatechol + nitrite + NAD+
-
Substrates: -
Products: -
Products: -
?
additional information
?
-
Substrates: no activity with 2,3-dinitrotoluene and 2,5-dinitrotoluene
Products: -
Products: -
?
additional information
?
-
-
Substrates: no activity with 2,3-dinitrotoluene and 2,5-dinitrotoluene
Products: -
Products: -
?
additional information
?
-
Substrates: no activity with 2,3-dinitrotoluene and 2,5-dinitrotoluene
Products: -
Products: -
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
2-mercaptoethanol
-
required for maximal activity, 292.3% activity at 1% (v/v)
phosphatidylethanolamine
-
144% activity at 0.1 mg per g wet weight of cells
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.38
with naphthalene as substrate, pH and temperature not specified in the publication
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.