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Information on EC 1.14.12.23 - nitroarene dioxygenase

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IUBMB Comments
This enzyme is a member of the naphthalene family of bacterial Rieske non-heme iron dioxygenases. It comprises a multicomponent system, containing a Rieske [2Fe-2S] ferredoxin, an NADH-dependent flavoprotein reductase (EC 1.18.1.3, ferredoxin---NAD+ reductase), and an alpha3beta3 oxygenase. The enzyme forms of a cis-dihydroxylated product that spontaneously rearranges to form a catechol with accompanying release of nitrite. It can typically act on many different nitroaromatic compounds, including chlorinated species. Enzymes found in different strains may have different substrate preferences. Requires Fe2+.
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UNIPROT: Q8RTL3
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Word Map
The enzyme appears in viruses and cellular organisms
Reaction Schemes
Synonyms
2ntdo, nitroarene dioxygenase, mntab, nbzaa, nbzab, nbzac, nbzad, cnbaa, cnbab, cnbac, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cnbA
-
-
-
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SYSTEMATIC NAME
IUBMB Comments
nitrobenzene,NADH:oxygen oxidoreductase (1,2-hydroxylating, denitrifying)
This enzyme is a member of the naphthalene family of bacterial Rieske non-heme iron dioxygenases. It comprises a multicomponent system, containing a Rieske [2Fe-2S] ferredoxin, an NADH-dependent flavoprotein reductase (EC 1.18.1.3, ferredoxin---NAD+ reductase), and an alpha3beta3 oxygenase. The enzyme forms of a cis-dihydroxylated product that spontaneously rearranges to form a catechol with accompanying release of nitrite. It can typically act on many different nitroaromatic compounds, including chlorinated species. Enzymes found in different strains may have different substrate preferences. Requires Fe2+.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
[2Fe-2S]-center
alpha subunit contains a Rieske [2Fe–2S]-center
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
Iron
active site of alpha subunit contains a mononuclear iron atom
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
beta-subunit of oxidase
UniProt
Manually annotated by BRENDA team
beta-subunit of oxidase
UniProt
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q8RTL3_9BURK
194
0
23086
TrEMBL
Secretory Pathway (Reliability: 1)
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme and in complex with nitrobenzene and 3-nitrotoluene, to 1.2 A, 1.55 A and 1.5 A resolution, respectively. Enzyme shows a mushroom-shaped alpha3beta3 hexamer. The catalytic subunit contains a Rieske iron-sulfur center and an active-site mononuclear iron atom. The substrate pocket contains an asparagine residue that forms a hydrogen bond to the nitro-group of the substrate, and orients the substrate in relation to the active-site mononuclear iron atom
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Friemann, R.; Ivkovic-Jensen, M.M.; Lessner, D.J.; Yu, C.L.; Gibson, D.T.; Parales, R.E.; Eklund, H.; Ramaswamy, S.
Structural insight into the dioxygenation of nitroarene compounds: the crystal structure of nitrobenzene dioxygenase
J. Mol. Biol.
348
1139-1151
2005
Comamonas sp. (Q8RTL3), Comamonas sp. JS7650 (Q8RTL3)
Manually annotated by BRENDA team