Information on EC 1.14.11.75 - 31-hydroxy-L-isoleucine 4-dioxygenase

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EC Tree 1 Oxidoreductases 1.14 Acting on paired donors, with incorporation or reduction of molecular oxygen 1.14.11 With 2-oxoglutarate as one donor, and incorporation of one atom of oxygen into each donor 1.14.11.75 31-hydroxy-L-isoleucine 4-dioxygenase

IUBMB Comments

Requires Fe²⁺ and ascorbate. The enzyme has been characterized from the bacterium Pantoea ananatis.

The expected taxonomic range for this enzyme is: Pantoea ananatis

Reaction Schemes

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31-hydroxy-L-isoleucine

2-oxoglutarate

(4S)-31,4-dihydroxy-L-isoleucine

succinate

CO2

Synonyms
4'-hydroxy-L-isoleucine 4-dioxygenase, HilB, L-4'-hydroxyisoleucine, 2-oxoglutarate: oxygen oxidoreductase (4-hydroxylating), L-isoleucine-4'-dioxygenase, more

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SYNONYM

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

4'-hydroxy-L-isoleucine 4-dioxygenase

incorrect

HilB

3 entries

L-4'-hydroxyisoleucine, 2-oxoglutarate: oxygen oxidoreductase (4-hydroxylating)

2 entries

L-isoleucine-4'-dioxygenase

2 entries

HilB

Pantoea ananatis

730346, 754661

HilB

Pantoea ananatis AJ13355

L-4'-hydroxyisoleucine, 2-oxoglutarate: oxygen oxidoreductase (4-hydroxylating)

Pantoea ananatis

730346

L-4'-hydroxyisoleucine, 2-oxoglutarate: oxygen oxidoreductase (4-hydroxylating)

Pantoea ananatis AJ13355

L-isoleucine-4'-dioxygenase

Pantoea ananatis

730346

L-isoleucine-4'-dioxygenase

Pantoea ananatis AJ13355

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REACTION

REACTION DIAGRAM

COMMENTARY

ORGANISM

UNIPROT

LITERATURE

31-hydroxy-L-isoleucine + 2-oxoglutarate + O2 = (4S)-31,4-dihydroxy-L-isoleucine + succinate + CO2

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SYSTEMATIC NAME

IUBMB Comments

31-hydroxy-L-isoleucine, 2-oxoglutarate:oxygen oxidoreductase (4S-hydroxylating)

Requires Fe2+ and ascorbate. The enzyme has been characterized from the bacterium Pantoea ananatis.

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SUBSTRATE

PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

Reversibility
r=reversible
ir=irreversible
?=not specified

(3S)-3'-hydroxy-L-isoleucine + 2-oxoglutarate + O2

(3S,4R)-3',4-dihydroxy-L-isoleucine + succinate + CO2

2 entries

31-hydroxy-L-isoleucine + 2-oxoglutarate + O2

(4S)-31,4-dihydroxy-L-isoleucine + succinate + CO2

2 entries

additional information

2 entries

(3S)-3'-hydroxy-L-isoleucine + 2-oxoglutarate + O2

(3S,4R)-3',4-dihydroxy-L-isoleucine + succinate + CO2

Pantoea ananatis

730346

Substrates: -
Products: -

(3S)-3'-hydroxy-L-isoleucine + 2-oxoglutarate + O2

(3S,4R)-3',4-dihydroxy-L-isoleucine + succinate + CO2

Pantoea ananatis AJ13355

730346

Substrates: -
Products: -

31-hydroxy-L-isoleucine + 2-oxoglutarate + O2

(4S)-31,4-dihydroxy-L-isoleucine + succinate + CO2

Pantoea ananatis

754661

Substrates: -
Products: -

31-hydroxy-L-isoleucine + 2-oxoglutarate + O2

(4S)-31,4-dihydroxy-L-isoleucine + succinate + CO2

Pantoea ananatis AJ13355

754661

Substrates: -
Products: -

additional information

Pantoea ananatis

730346

Substrates: L-isoleucine 3'-hydroxylase HilA and 3'-hydroxy-L-isoleucine 4-hydroxylase HilB catalyze the consecutive hydroxylation of L-isoleucine at the C-3' and C-4 positions, respectively, to synthesize L-3',4-dihydroxyisoleucine. When expressed without HilA, HilB acts as a minimally effective L-isoleucine-4-dioxygenase
Products: -

additional information

Pantoea ananatis AJ13355

730346

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NATURAL SUBSTRATE

NATURAL PRODUCT

REACTION DIAGRAM

ORGANISM

UNIPROT

LITERATURE

COMMENTARY

REVERSIBILITY
r=reversible
ir=irreversible
?=not specified

(3S)-3'-hydroxy-L-isoleucine + 2-oxoglutarate + O2

(3S,4R)-3',4-dihydroxy-L-isoleucine + succinate + CO2

2 entries

additional information

2 entries

(3S)-3'-hydroxy-L-isoleucine + 2-oxoglutarate + O2

(3S,4R)-3',4-dihydroxy-L-isoleucine + succinate + CO2

Pantoea ananatis

730346

Substrates: -
Products: -

(3S)-3'-hydroxy-L-isoleucine + 2-oxoglutarate + O2

(3S,4R)-3',4-dihydroxy-L-isoleucine + succinate + CO2

Pantoea ananatis AJ13355

730346

Substrates: -
Products: -

additional information

Pantoea ananatis

730346

additional information

Pantoea ananatis AJ13355

730346

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METALS and IONS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Fe2+

Fe2+

required

Fe2+

dependent on

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only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks include all textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks + FRENDAco-occurrence of enzyme names and organism names (less precise)

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ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

Pantoea ananatis

730346, 754661

brenda

Pantoea ananatis AJ13355

730346, 754661

brenda

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GENERAL INFORMATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

physiological function

2 entries

physiological function

Pantoea ananatis

enzymes HilA and HilB catalyze the consecutive hydroxylation of L-isoleucine at the C-31 and C-4 positions, respectively, to synthesize L-31,4-dihydroxyisoleucine

754661

physiological function

Pantoea ananatis AJ13355

enzymes HilA and HilB catalyze the consecutive hydroxylation of L-isoleucine at the C-31 and C-4 positions, respectively, to synthesize L-31,4-dihydroxyisoleucine

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UNIPROT

ENTRY NAME

ORGANISM

NO. OF AA

NO. OF TRANSM. HELICES

MOLECULAR WEIGHT[Da]

SOURCE

SEQUENCE

LOCALIZATION PREDICTION

The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).

A0A0H3L116 pBLAST

HILB_PANAA

Pantoea ananatis (strain AJ13355)

247

28263

Swiss-Prot

Show Sequence

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MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

30000

Pantoea ananatis

x * 30000, SDS-PAGE

730346

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SUBUNIT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

2 entries

Pantoea ananatis

x * 30000, SDS-PAGE

730346

Pantoea ananatis AJ13355

x * 30000, SDS-PAGE

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CLONED (Commentary)

ORGANISM

UNIPROT

LITERATURE

expressed in Escherichia coli BL21(DE3) cells

Pantoea ananatis

730346

expression in Escherichia coli

Pantoea ananatis

754661

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REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

730346

Smirnov, S.; Sokolov, P.; Kotlyarova, V.; Samsonova, N.; Kodera, T.; Sugiyama, M.; Torii, T.; Hibi, M.; Shimizu, S.; Yokozeki, K.; Ogawa, J.

A novel L-isoleucine-4'-dioxygenase and L-isoleucine dihydroxylation cascade in Pantoea ananatis

MicrobiologyOpen

471-481

2013

Pantoea ananatis, Pantoea ananatis AJ13355

23554367

brenda

754661

Smirnov, S.; Sokolov, P.; Kotlyarova, V.; Samsonova, N.; Kodera, T.; Sugiyama, M.; Torii, T.; Hibi, M.; Shimizu, S.; Yokozeki, K.; Ogawa, J.

A novel L-isoleucine-4'-dioxygenase and L-isoleucine dihydroxylation cascade in Pantoea ananatis

MicrobiologyOpen

471-481

2013

Pantoea ananatis, Pantoea ananatis AJ13355

23554367

brenda

Entries 1 - 20 of 2

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EXTERNAL LINKS (specific for EC-Number 1.14.11.75)

ExplorEnz (official portal for IUBMB Enzyme Nomenclature)

Expasy Enzyme Nomenclature Database

KEGG

SABIO-RK

NCBI: PubMed, Protein, Nucleotide, Structure, Gene, OMIM

Enzyme Nomenclature (alternative site)

UniProt

PDB

PROSITE Database of protein families and domains

InterPro (database of protein families, domains and functional sites)

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