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Information on EC 1.14.11.15 - gibberellin 3beta-dioxygenase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9ZT84
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1.14.11.15 gibberellin 3beta-dioxygenaseIUBMB Comments
Requires Fe2+ and ascorbate.
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Word Map
- 1.14.11.15
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20-oxidase
- ga1
- dellas
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phytochrome
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ga-deficient
- paclobutrazol
- ent-kaurene
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atga20ox1
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ga-dependent
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ent-copalyl
- ga2ox2
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ent-kaurenoic
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ga-mediated
The taxonomic range for the selected organisms is: Arabidopsis thaliana
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
ga3ox, ga 3-oxidase, ga3ox1, atga3ox1, ga 3beta-hydroxylase, ga3-oxidase, osga3ox1, gibberellin 3-oxidase, zmga3ox2, mtga3ox1, 
more
topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
(giberrellin-20),2-oxoglutarate: oxygen oxidoreductase (3beta-hydroxylating)
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GA 3-oxidase
gibberellin 3beta dioxygenase
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gibberellin 3beta-hydroxylase
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oxygenase, gibberellin 3beta-di-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
redox reaction
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oxidation
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reduction
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SYSTEMATIC NAME
IUBMB Comments
(gibberellin-20),2-oxoglutarate:oxygen oxidoreductase (3beta-hydroxylating)
Requires Fe2+ and ascorbate.
CAS REGISTRY NUMBER
COMMENTARY
116036-68-1
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
gibberellin a20 + 2-oxoglutarate + O2
gibberellin a1 + succinate + CO2
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?
gibberellin 20 + 2-oxoglutarate + O2
gibberellin 1 + succinate + CO2
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?
additional information
?
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gibberellin 3-oxidase expression in cold-imbimbed seeds is significantly lower in the dark than in white light, 22°C
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?
gibberellin a20 + 2-oxoglutarate + O2
gibberellin a1 + succinate + CO2
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?
gibberellin a20 + 2-oxoglutarate + O2
gibberellin a1 + succinate + CO2
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-
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?
gibberellin a9 + 2-oxoglutarate + O2
gibberellin a4 + succinate + CO2
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-
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?
gibberellin a9 + 2-oxoglutarate + O2
gibberellin a4 + succinate + CO2
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-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
gibberellin a20 + 2-oxoglutarate + O2
gibberellin a1 + succinate + CO2
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-
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?
gibberellin 20 + 2-oxoglutarate + O2
gibberellin 1 + succinate + CO2
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?
additional information
?
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gibberellin 3-oxidase expression in cold-imbimbed seeds is significantly lower in the dark than in white light, 22°C
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?
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
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150 mM NaCl represses the gibberellin 3-oxidase 1 gene in the wild-type and in the NTL8-deficient mutant (NTL8 acts downstream of the gibberellin biosynthesis), repression is present but less significant in aba3-1 mutant, deficient in the stress hormone abscisic acid (independent pathway dominates), expression of GA3ox1 is recovered after a longer incubation in NaCl and leads to germination
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
additional information
the enzyme is inhibited by Bacillus subtilis strain IJ-31 and hydrocinnamic acid, which is one of the allelochemicals produced by Bacillus subtilis strain IJ-31
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.00082
gibberellin a20
in 100 mM Tris-HCl pH 7.9 containing 4 mM ascorbate, 4 mM DTT, 4 mM 2-oxoglutarate, 0.5 mM FeSO4, 2 mg/ml bovine serum albumin and 1 mg/ml catalase
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.0216
wild type enzyme from soluble cell fraction, in 100 mM Tris-HCl pH 7.9 containing 4 mM ascorbate, 4 mM DTT, 4 mM 2-oxoglutarate, 0.5 mM FeSO4, 2 mg/ml bovine serum albumin and 1 mg/ml catalase
0.259
wild type enzyme after 12fold purification, in 100 mM Tris-HCl pH 7.9 containing 4 mM ascorbate, 4 mM DTT, 4 mM 2-oxoglutarate, 0.5 mM FeSO4, 2 mg/ml bovine serum albumin and 1 mg/ml catalase
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
treatment with either Bacillus subtilis strain IJ-31 culture extract or its allelochemicals, like hydrocinnamic acid, results in significant down-regulation of XTR9 gene expression, encoding xyloglucan endotransglucosylase/hydrolase protein 14, which is involved in plant cell elongation by cleavage of loadbearing cross-links between cellulose microfibrils, leading to wall-loosening and enabling elongation to occur, in Arabidopsis thaliana, as well as inhibition of gibberellin 3beta-hydroxxylase
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable).
The reliability class of the localization prediction ranges from 1 (very reliable) to 5 (not reliable). G3OX2_ARATH
347
0
38782
Swiss-Prot
other Location (Reliability: 4)
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
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transgenic plant lines with green fluorescence protein-anti gibberellin24 antibody fusion construct (GFP-scFv), called A24-1, 2, and 3, up-regulation in the transgenic plant line A24-2 enhances biosynthesis of gibberellin 24 which accumulates compared to wild-type. GFP-scFv reduces rosette leaf development, delays flower induction, reduces stem elongation of main culm, especially in the early stage of inflorescence growth, later stem elongation of the main culm and lateral shoot elongation is much less affected
additional information
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transgenic plants overexpressing an active NTL8 form and T-DNA insertional ntl8-1 mutant, under standard conditions, germinate with the same rate, under high salinity stress (150 mM NaCl) the germination of the NTL8 overexpressing mutant is considerably reduced while the NTL8-deficient mutant is less sensitive, independent of absiasic acid (ABA), NTL8-deficient mutant is not impaired by gibberellin biosynthetic inhibitor paclabutrazol (PAC), gibberellin downregulates NTL8 lowering its threshold for germination
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
immobilised metal affinity chromatography and Superdex 200 gel filtration
recombinant maltose-binding protein-fused enzyme from Escherichia coli by amylose affinity chromatography
rosette leaves are frozen in liquid nitrogen and ground to a fine powder, protein extracted with 50 mM Tris-HCl, pH 8.0, containing 200 mM NaCl, 5 mM EDTA, 0.1% Tween 20 and a proteinase inhibitor mixture, SDS-PAGE
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transgenic plants are ground in liquid nitrogen, total cellular extracts suspended in 1X SDS-PAGE sample loading buffer
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
for the NTL8 complementation test, a sequence containing the NTL8 gene with its promoter is subcloned into pKGWFS7 Gateway vector, for histochemical staining an NTL8 promoter region is transcriptionally fused to the GUS-coding sequence, the fusion product transformed into Arabidopsis
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gene GA4, expression as maltose-binding protein-fusion protein in Escherichia coli
the anti gibberellin 24 single monoclonal antibody gene (anti-GA24 scFv) is PCR-amplified with plasmid pH1L24B and introduced into the binary vector pBI-ER-D, transferred into Agrobacterium tumefaciens strain LBA4404 to transform Arabidospsis thaliana, the antibody is fused with the green fluorescence protein (GFP), a c-myc epitope tag and KDEL ER-retention signal
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yamaguchi, S.; Smith, M.W.; Brown, R.G.S.; Kamiya, Y.; Sun, T.P.
Phytochrome regulation and differential expression of gibberellin 3beta-hydroxylase genes in germinating Arabidopsis seeds
Plant Cell
10
2115-2126
1998
Arabidopsis thaliana (Q9ZT84)
Lester, D.R.; Phillips, A.; Hedden, P.; Andersson, I.
Purification and kinetic studies of recombinant gibberellin dioxygenases
BMC Plant Biol.
5
19
2005
Arabidopsis thaliana (Q9C971), Arabidopsis thaliana
Mitchum, M.G.; Yamaguchi, S.; Hanada, A.; Kuwahara, A.; Yoshioka, Y.; Kato, T.; Tabata, S.; Kamiya, Y.; Sun, T.P.
Distinct and overlapping roles of two gibberellin 3-oxidases in Arabidopsis development
Plant J.
45
804-818
2006
Arabidopsis thaliana
Kim, S.G.; Lee, A.K.; Yoon, H.K.; Park, C.M.
A membrane-bound NAC transcription factor NTL8 regulates gibberellic acid-mediated salt signaling in Arabidopsis seed germination
Plant J.
55
77-88
2008
Arabidopsis thaliana
Urakami, E.; Yamaguchi, I.; Asami, T.; Conrad, U.; Suzuki, Y.
Immunomodulation of gibberellin biosynthesis using an anti-precursor gibberellin antibody confers gibberellin-deficient phenotypes
Planta
228
863-873
2008
Arabidopsis thaliana
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