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Information on EC 1.14.11.15 - gibberellin 3beta-dioxygenase and Organism(s) Triticum aestivum and UniProt Accession Q3I410

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IUBMB Comments
Requires Fe2+ and ascorbate.
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This record set is specific for:
Triticum aestivum
UNIPROT: Q3I410
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The taxonomic range for the selected organisms is: Triticum aestivum
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
ga3ox, ga 3-oxidase, ga3ox1, atga3ox1, ga3-oxidase, ga 3beta-hydroxylase, osga3ox1, gibberellin 3-oxidase, zmga3ox2, mtga3ox1, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
GA 3-oxidase 2
-
gibberellin 3-beta-dioxygenase 2-2
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gibberellin 3-oxidase 2
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(giberrellin-20),2-oxoglutarate: oxygen oxidoreductase (3beta-hydroxylating)
-
-
-
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gibberellin 3-beta-dioxygenase 2-1
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gibberellin 3-beta-dioxygenase 2-3
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gibberellin 3-oxidase 2
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gibberellin 3beta dioxygenase
-
-
-
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gibberellin 3beta-hydroxylase
-
-
-
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oxygenase, gibberellin 3beta-di-
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
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oxidation
-
-
-
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reduction
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
(gibberellin-20),2-oxoglutarate:oxygen oxidoreductase (3beta-hydroxylating)
Requires Fe2+ and ascorbate.
CAS REGISTRY NUMBER
COMMENTARY hide
116036-68-1
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
gibberellin a20 + 2-oxoglutarate + O2
gibberellin a1 + succinate + CO2
show the reaction diagram
-
-
-
?
gibberellin a9 + 2-oxoglutarate + O2
gibberellin a4 + succinate + CO2
show the reaction diagram
-
-
-
?
gibberellin a20 + 2-oxoglutarate + O2
gibberellin a1 + succinate + CO2
show the reaction diagram
-
-
-
?
gibberellin a9 + 2-oxoglutarate + O2
gibberellin a4 + succinate + CO2
show the reaction diagram
-
-
-
?
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.002024
gibberellin a20
in 100 mM Tris-HCl at pH 7.5
0.001
gibberellin a9
in 100 mM Tris-HCl at pH 7.5
0.002024
gibberellin a20
in 100 mM Tris-HCl at pH 7.5
0.001
gibberellin a9
in 100 mM Tris-HCl at pH 7.5
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gibberellin 3-beta-dioxygenase 2-2
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
in internodes, as well as nodes and the ear of the elongating stem
Manually annotated by BRENDA team
in internodes, as well as nodes and the ear of the elongating stem
Manually annotated by BRENDA team
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
G3O22_WHEAT
370
0
40355
Swiss-Prot
other Location (Reliability: 5)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
high performance liquid chromatography
high performance liquid chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli strain BL21
expressed in Escherichia coli strain BL21
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Appleford, N.E.; Evans, D.J.; Lenton, J.R.; Gaskin, P.; Croker, S.J.; Devos, K.M.; Phillips, A.L.; Hedden, P.
Function and transcript analysis of gibberellin-biosynthetic enzymes in wheat
Planta
223
568-582
2006
Triticum aestivum (Q3I409), Triticum aestivum (Q3I410), Triticum aestivum (Q3I411)
Manually annotated by BRENDA team