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Information on EC 1.14.11.13 - gibberellin 2beta-dioxygenase and Organism(s) Oryza sativa and UniProt Accession P93771
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1.14.11.13 gibberellin 2beta-dioxygenaseIUBMB Comments
Also acts on a number of other gibberellins.
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Word Map
- 1.14.11.13
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dwarf
- ga1
- ga20ox
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3-oxidase
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ga-deficient
- dellas
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psga3ox1
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semi-dwarfism
- paclobutrazol
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2beta-hydroxylation
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ga-deactivating
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ga-biosynthesis
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2-oxidation
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ent-kaurenoic
- agriculture
- biofuel production
The taxonomic range for the selected organisms is: Oryza sativa
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota
Synonyms
2-oxoglutarate-dependent dioxygenase, ga2ox, ga 2-oxidase, psga2ox1, psga2ox2, gibberellin 2-oxidase, ga2ox1, ga2ox7, ga2ox2, ga2ox3, 
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topSYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
gibberellin 2-oxidase
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4 partially characterized gibberellin 2-oxidases (GA2ox) (GA2ox1 to GA2ox4), 2 uncharacterized GA2oxs (GA2ox5 and GA2ox6), 4 other (GA2oxs GA2ox7 to GA2ox9, GA2ox10 is probably a pseudogene)
gibberellin 2beta-hydroxylase
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
redox reaction
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-
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oxidation
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-
-
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reduction
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SYSTEMATIC NAME
IUBMB Comments
(gibberellin-1),2-oxoglutarate:oxygen oxidoreductase (2beta-hydroxylating)
Also acts on a number of other gibberellins.
CAS REGISTRY NUMBER
COMMENTARY
85713-20-8
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
gibberellin 1 + 2-oxoglutarate + O2
2beta-hydroxygibberellin 1 + succinate + CO2
-
-
-
?
gibberellin 20 + 2-oxoglutarate + O2
gibberellin 29 + succinate + CO2
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-
?
gibberellin 4 + 2-oxoglutarate + O2
gibberellin 34 + succinate + CO2
-
-
?
gibberellin 44 + 2-oxoglutarate + O2
gibberellin 98 + succinate + CO2
-
-
?
gibberellin 9 + 2-oxoglutarate + O2
gibberellin 51 + succinate + CO2
-
-
?
gibberellin + 2-oxoglutarate + O2
2beta-hydroxygibberellin + succinate + CO2
-
catabolization of biologically active gibberellin
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-
?
gibberellin 1 + 2-oxoglutarate + O2
gibberellin 8 + succinate + CO2
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-
-
?
gibberellin 12 + 2-oxoglutarate + O2
gibberellin 110 + succinate + CO2
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GA2ox5 and GA2ox6
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-
?
gibberellin 20 + 2-oxoglutarate + O2
gibberellin 29 + succinate + CO2
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-
-
?
gibberellin 53 + 2-oxoglutarate + O2
gibberellin 97 + succinate + CO2
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GA2ox5 and GA2ox6
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-
?
gibberellin 9 + 2-oxoglutarate + O2
gibberellin 51 + succinate + CO2
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-
-
?
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
gibberellin + 2-oxoglutarate + O2
2beta-hydroxygibberellin + succinate + CO2
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catabolization of biologically active gibberellin
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-
?
ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
SOURCE
the enzyme is primarily expressed in rice culm basal internodes
the enzyme is primarily expressed in rice culm basal internodes
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
metabolism
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gibberellin 2-oxidase plays a key role in the GA catabolic pathway through 2beta-hydroxylation
physiological function
physiological function
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gibberellin 2-oxidase plays a key role in the GA catabolic pathway through 2beta-hydroxylation, activation of gibberellin 2-oxidase 6 decreases active gibberellin levels and creates a dominant semi-dwarf phenotype in rice, overview. GA biosynthesis inhibitor, paclobutrazol, positively regulates the OsGA2ox6 gene. The H032 mutant retains normal flowering and seed production, H032 mutant phenotype, overview
physiological function
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key enzyme that inactivates endogenous gibberellins and their precursors. The enzyme GA2ox6 regulates plant architecture
physiological function
the enzyme is primarily expressed in rice culm basal internodes, regulating the basal internode length
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
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mutants overexpressing GA2ox9ACT, GA2ox5delta335-341ACT, and GA2ox6, motif III of the conserved motifs characteristic for C20 GA2oxs is necessary for C20 GA2ox activity, motif III is present in rice GA2ox5, GA2ox6, and GAsox9
additional information
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isolation of a CaMV 35S-enhancer activation tagged mutant, H032, showing an increased level of OsGA2ox6 mRNA compared to the wild-type plants. RNAi and ectopic expression analysis of OsGA2ox6 show that the dwarf trait and the decreased levels of bioactive GAs in the H032 mutant are a result of the upregulation of the OsGA2ox6 gene. The H032 mutant retains normal flowering and seed production
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Escherichia coli with recombinant enzyme are centrifuged, resuspended in a BugBuster Protein Extraction Reagent, centrifuged, supernatants eluted through a GST-Bind resin with 50 mM Tris buffer, pH 8.0, containing 10 mM reduced glutathione, storage at -80°C
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA isolation of OsGA2ox1, heterologously expressed in Escherichia coli, ectopic expression in transgenic rice
gene OsGA2ox6, DNA and amino acid sequence determination and analysis, sequence comparison, semi-quantitative real-time RT-PCR analysis
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PCR-amplifiction of cDNA, inserted via pGEM-T Easy cloning vector and pAHC18 into pCAMBIA1301 and transferred into Agrobacterium tumefaciens (strain EHA105) and used to transform rice and tobacco (Nicotiana tabacum), or for activity assays subcloned into pGEX-5X expression vector to transform Escherichia coli BL21(DE3)
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
GA biosynthesis inhibitor, paclobutrazol, positively regulates the OsGA2ox6 gene
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
agriculture
agriculture
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breeding plants with reduced height, increased root biomass, normal flowering and seed production by overexpression of C20 gibberellin 2-oxidases: first overexpression of GA2ox9ACT mutant generates semidwarf rice with only slightly reduced grain weight and fertility, increased tiller number (by 22%) compared to wild-type, second overexpression of C20 GA2oxs with defective motif III, such as GA2ox5delta335-341ACT mutant, generates a semidwarf rice variety with reduced grain weight (by 16%) and fertility (by 12%) and twofold increased tiller number, third overexpression of a selected C20 GA2ox gene, such as GA2ox6 with less effect on plant growth under the control of a weak promoter could be beneficial without sacrificing seed production
agriculture
applications of the SBI(SV14) allele in rice breeding are an efficient strategy to develop elite rice varieties with improved lodging resistance and increased yield
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Sakamoto, T.; Kobayashi, M.; Itoh, H.; Tagiri, A.; Kayano, T.; Tanaka, H.; Iwahori, S.; Matsuoka, M.
Expression of a gibberellin 2-oxidase gene around the shoot apex is related to phase transition in rice
Plant Physiol.
125
1508-1516
2001
Arabidopsis sp., Oryza sativa (P93771), Oryza sativa, Phaseolus coccineus, Pisum sativum
Lo, S.F.; Yang, S.Y.; Chen, K.T.; Hsing, Y.I.; Zeevaart, J.A.; Chen, L.J.; Yu, S.M.
A novel class of gibberellin 2-oxidases control semidwarfism, tillering, and root development in rice
Plant Cell
20
2603-2618
2008
Oryza sativa
Huang, J.; Tang, D.; Shen, Y.; Qin, B.; Hong, L.; You, A.; Li, M.; Wang, X.; Yu, H.; Gu, M.; Cheng, Z.
Activation of gibberellin 2-oxidase 6 decreases active gibberellin levels and creates a dominant semi-dwarf phenotype in rice (Oryza sativa L.)
J. Genet. Genomics
37
23-36
2010
Oryza sativa
Liu, C.; Zheng, S.; Gui, J.; Fu, C.; Yu, H.; Song, D.; Shen, J.; Qin, P.; Liu, X.; Han, B.; Yang, Y.; Li, L.
Shortened basal internodes encodes a gibberellin 2-oxidase and contributes to lodging resistance in rice
Mol. Plant
11
288-299
2018
Oryza sativa (A0A2H5BXP6), Oryza sativa (A0A2H5BXP8), Oryza sativa
Lo, S.F.; Ho, T.D.; Liu, Y.L.; Jiang, M.J.; Hsieh, K.T.; Chen, K.T.; Yu, L.C.; Lee, M.H.; Chen, C.Y.; Huang, T.P.; Kojima, M.; Sakakibara, H.; Chen, L.J.; Yu, S.M.
Ectopic expression of specific GA2 oxidase mutants promotes yield and stress tolerance in rice
Plant Biotechnol. J.
15
850-864
2017
Oryza sativa
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