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IUBMB Comments Isolated from the bacterium Streptomyces roseochromogenes DS 12976. A bifunctional enzyme involved in clorobiocin biosynthesis that also catalyses the activity of EC 1.13.11.83 , 4-hydroxy-3-prenylphenylpyruvate oxygenase.
Reaction Schemes
4-hydroxy-3-prenylmandelate
+
=
4-hydroxy-3-prenylbenzoate
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Synonyms novR ,
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3-dimethylallyl-4-hydroxymandelate:oxygen oxidoreductase (3-dimethylallyl-4-hydroxybenzoate forming)
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CloR
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novR
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4-hydroxy-3-prenylmandelate + O2 = 4-hydroxy-3-prenylbenzoate + CO2 + H2O
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MetaCyc
4-hydroxy-3-prenylbenzoate biosynthesis
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4-hydroxy-3-prenylmandelate:oxygen oxidoreductase (4-hydroxy-3-prenylbenzoate forming)
Isolated from the bacterium Streptomyces roseochromogenes DS 12976. A bifunctional enzyme involved in clorobiocin biosynthesis that also catalyses the activity of EC 1.13.11.83, 4-hydroxy-3-prenylphenylpyruvate oxygenase.
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3-dimethylallyl-4-hydroxymandelate + O2
3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
3-dimethylallyl-4-hydroxymandelate + O2
3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
Substrates: -
?
3-dimethylallyl-4-hydroxymandelate + O2
3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
Substrates: -
?
3-dimethylallyl-4-hydroxymandelate + O2
3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
Substrates: -
?
3-dimethylallyl-4-hydroxymandelate + O2
3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
Substrates: -
?
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3-dimethylallyl-4-hydroxymandelate + O2
3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
3-dimethylallyl-4-hydroxymandelate + O2
3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
Substrates: -
?
3-dimethylallyl-4-hydroxymandelate + O2
3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
Substrates: -
?
3-dimethylallyl-4-hydroxymandelate + O2
3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
Substrates: -
?
3-dimethylallyl-4-hydroxymandelate + O2
3-dimethylallyl-4-hydroxybenzoate + CO2 + H2O
Substrates: -
?
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Fe2+
a non-heme iron enzyme, Fe2+ is required for enzyme activity. Replacement of Fe2+ with other monovalent or divalent metal ions (1 mM Cu+, Zn2+, Mg2+, or Mn2+) results in almost complete (95-98%) loss of enzymatic activity
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additional information
the enzyme is not stimulated by addition of 2-oxoglutarate
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UniProt
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UniProt
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UniProt
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UniProt
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Highest Expressing Human Cell Lines
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CLOR_STRRC
277
0
30496
Swiss-Prot
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x * 30500, calculated from amino acid sequence
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x * 30500, calculated from amino acid sequence
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glutathione-Sepharose column chromatography
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expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli XL-1 Blue MRF' cells
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Pojer, F.; Kahlich, R.; Kammerer, B.; Li, S.M.; Heide, L.
CloR, a bifunctional non-heme iron oxygenase involved in clorobiocin biosynthesis
J. Biol. Chem.
278
30661-30668
2003
Streptomyces roseochromogenus (Q8GHB1), Streptomyces roseochromogenus DS 12976 (Q8GHB1)
brenda
Pojer, F.; Li, S.M.; Heide, L.
Molecular cloning and sequence analysis of the clorobiocin biosynthetic gene cluster new insights into the biosynthesis of aminocoumarin antibiotics
Microbiology
148
3901-3911
2002
Streptomyces roseochromogenus subsp. oscitans (Q8GHB1), Streptomyces roseochromogenus subsp. oscitans DS 12.976 (Q8GHB1)
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