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Information on EC 1.13.11.58 - linoleate 9S-lipoxygenase and Organism(s) Oryza sativa and UniProt Accession Q76I22

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IUBMB Comments
Contains nonheme iron. A common plant lipoxygenase that oxidizes linoleate and alpha-linolenate, the two most common polyunsaturated fatty acids in plants, by inserting molecular oxygen at the C9 position with (S)-configuration. The enzyme plays a physiological role during the early stages of seedling growth. The enzyme from Arabidopsis thaliana shows comparable activity towards linoleate and linolenate . EC 1.13.11.12 (linoleate 13S-lipoxygenase) catalyses a similar reaction at another position of these fatty acids.
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Oryza sativa
UNIPROT: Q76I22
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Word Map
The taxonomic range for the selected organisms is: Oryza sativa
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria
Synonyms
9-lipoxygenase, zmlox3, r9-lox1, zmlox5, calox1, atlox1, epidermal lipoxygenase-3, ghlox1, nb-9-lox, linoleate 9s-lipoxygenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
linoleate 9S-lipoxygenase 2
UniProt
PATHWAY SOURCE
PATHWAYS
-
-, -, -, -, -, -
SYSTEMATIC NAME
IUBMB Comments
linoleate:oxygen 9-oxidoreductase
Contains nonheme iron. A common plant lipoxygenase that oxidizes linoleate and alpha-linolenate, the two most common polyunsaturated fatty acids in plants, by inserting molecular oxygen at the C9 position with (S)-configuration. The enzyme plays a physiological role during the early stages of seedling growth. The enzyme from Arabidopsis thaliana shows comparable activity towards linoleate and linolenate [4]. EC 1.13.11.12 (linoleate 13S-lipoxygenase) catalyses a similar reaction at another position of these fatty acids.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
alpha-linolenate + O2
(9S,10E,12Z,15Z)-9-hydroperoxy-10,12,15-octadecatrienoate
show the reaction diagram
comparable oxygenase activity with either linoleic acid or linolenic acid
more than 96% of the substrate is converted to the the 9-positional hydroperoxide
-
?
linoleate + O2
(9S,10E,12Z)-9-hydroperoxy-10,12-octadecadienoate
show the reaction diagram
comparable oxygenase activity with either linoleic acid or linolenic acid
-
-
?
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5.3 - 6.5
pH 5.3: about 80% of maximal activity, pH 6.5: about 40% of maximal activity
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
15 - 30
15°C: about 90% of maximal activity, 30°C: about 40% of maximal activity
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
L. cv. Nipponbare
SwissProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
the expression level of the r9-LOX1 gene is higher in imbibed seeds rather than developing seeds
Manually annotated by BRENDA team
additional information
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene LOX1.1, DNA and amino acid sequence determination and analysis, realtime PCR expression analysis, the gene shows a cultivar/tissue specific expression
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
the enzyme could be useful for in vitro synthesis of 9,10-ketol-octadecadienoic acid which is expected to be an ornamentally useful flower inducer when applied exogenously
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Mizuno, K.; Iida, T.; Takano, A.; Yokoyama, M.; Fujimura, T.
A new 9-lipoxygenase cDNA from developing rice seeds
Plant Cell Physiol.
44
1168-1175
2003
Oryza sativa (Q76I22), Oryza sativa
Manually annotated by BRENDA team
Roychowdhury, M.; Li, X.; Qi, H.; Li, W.; Sun, J.; Huang, C.; Wu, D.
Functional characterization of 9-/13-LOXs in rice and silencing their expressions to improve grain qualities
BioMed Res. Int.
2016
4275904
2016
Oryza sativa (P29250), Oryza sativa, Oryza sativa Japonica Group (Q76I22)
Manually annotated by BRENDA team