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Information on EC 1.13.11.4 - gentisate 1,2-dioxygenase

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EC Tree
IUBMB Comments
Requires Fe2+.
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This record set is specific for:
UNIPROT: Q8NLB9
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Word Map
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
gentisate 1,2-dioxygenase, salicylate 1,2-dioxygenase, gentisate dioxygenase, gdosp, gdo-ii, gentisate-1,2-dioxygenase, gentisate oxygenase, 2,5-dihydroxybenzoate dioxygenase, gentisate:oxygen 1,2-oxidoreductase (decyclizing), p25x gentisate 1,2-dioxygenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
2,5-dihydroxybenzoate dioxygenase
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gentisate dioxygenase
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gentisate oxygenase
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gentisic acid oxidase
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oxygenase, gentisate 1,2-di-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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oxidation
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reduction
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SYSTEMATIC NAME
IUBMB Comments
gentisate:oxygen 1,2-oxidoreductase (decyclizing)
Requires Fe2+.
CAS REGISTRY NUMBER
COMMENTARY hide
9029-48-5
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2,5-dihydroxybenzoate + O2
maleylpyruvate
show the reaction diagram
the enzyme is highly specific for 2,5-dihydroxybenzoate
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-
?
5-aminosalicylate + O2
?
show the reaction diagram
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-
?
5-fluorosalicylate + O2
?
show the reaction diagram
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-
-
?
additional information
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2,5-dihydroxybenzoate + O2
maleylpyruvate
show the reaction diagram
the enzyme is highly specific for 2,5-dihydroxybenzoate
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-
?
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.13
2,5-Dihydroxybenzoate
at pH 8.0 and 25°C
2.7
5-Aminosalicylate
at pH 8.0 and 25°C
0.99
5-Fluorosalicylate
at pH 8.0 and 25°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
2310
2,5-Dihydroxybenzoate
at pH 8.0 and 25°C
1.1
5-Aminosalicylate
at pH 8.0 and 25°C
0.4
5-Fluorosalicylate
at pH 8.0 and 25°C
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q8NLB9_CORGL
Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / BCRC 11384 / JCM 1318 / LMG 3730 / NCIMB 10025)
375
0
41375
TrEMBL
other Location (Reliability: 3)
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A112D
the mutant gains the ability to oxidize salicylate and several other monohydroxylated substrates
A112G
the mutant gains the ability to oxidize salicylate and several other monohydroxylated substrates
A112I
the mutant gains the ability to oxidize salicylate and several other monohydroxylated substrates
A112S
the mutant gains the ability to oxidize salicylate and several other monohydroxylated substrates
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Ni-NTA column chromatography
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Eppinger, E.; Stolz, A.
Expansion of the substrate range of the gentisate 1,2-dioxygenase from Corynebacterium glutamicum for the conversion of monohydroxylated benzoates
Protein Eng. Des. Sel.
30
57-65
2017
Corynebacterium glutamicum (Q8NLB9), Corynebacterium glutamicum, Corynebacterium glutamicum ATCC 13032 (Q8NLB9)
Manually annotated by BRENDA team