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Information on EC 1.11.1.11 - L-ascorbate peroxidase and Organism(s) Hordeum vulgare and UniProt Accession Q945R5

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EC Tree
     1 Oxidoreductases
         1.11 Acting on a peroxide as acceptor
             1.11.1 Peroxidases
                1.11.1.11 L-ascorbate peroxidase
IUBMB Comments
A heme protein. Oxidizes ascorbate and low molecular weight aromatic substrates. The monodehydroascorbate radical produced is either directly reduced back to ascorbate by EC 1.6.5.4 [monodehydroascorbate reductase (NADH)] or undergoes non-enzymic disproportionation to ascorbate and dehydroascorbate.
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This record set is specific for:
Hordeum vulgare
UNIPROT: Q945R5
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Word Map
The taxonomic range for the selected organisms is: Hordeum vulgare
The enzyme appears in selected viruses and cellular organisms
Reaction Schemes
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Synonyms
ascorbate peroxidase, apex2, cytosolic ascorbate peroxidase, lmapx, ascorbate peroxidase 2, l-ascorbate peroxidase, ascorbate peroxidase 1, ascorbic acid peroxidase, osapx8, osapx2, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ascorbate peroxidase
-
ascorbate peroxidase
ascorbic acid peroxidase
-
-
-
-
HvAPX1
-
-
L-ascorbic acid peroxidase
-
-
-
-
L-ascorbic acid-specific peroxidase
-
-
-
-
peroxidase, ascorbate
-
-
-
-
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
-
-
-
-
oxidation
reduction
peroxidation
-
-
-
-
SYSTEMATIC NAME
IUBMB Comments
L-ascorbate:hydrogen-peroxide oxidoreductase
A heme protein. Oxidizes ascorbate and low molecular weight aromatic substrates. The monodehydroascorbate radical produced is either directly reduced back to ascorbate by EC 1.6.5.4 [monodehydroascorbate reductase (NADH)] or undergoes non-enzymic disproportionation to ascorbate and dehydroascorbate.
CAS REGISTRY NUMBER
COMMENTARY hide
72906-87-7
-
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
L-ascorbate + H2O2
dehydroascorbate + H2O
show the reaction diagram
-
-
-
?
L-ascorbate + H2O2
dehydroascorbate + H2O
show the reaction diagram
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
L-ascorbate + H2O2
dehydroascorbate + H2O
show the reaction diagram
-
-
-
-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
7
-
activity assay
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
-
UniProt
Manually annotated by BRENDA team
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
additional information
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
Q945R5_HORVU
256
0
27639
TrEMBL
other Location (Reliability: 4)
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Arabidopsis thaliana leaf extracts are prepared
-
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
transgenic Arabidopsis thaliana constitutively overexpressing HvAPX1 under control of the CaMv 35S promoter
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
no ascorbate peroxidase activity is present in mature seeds but activity is detected after 24 h post imbibition and increases 14fold up to 144 h post imbibition
HvAPX1-overexpressing transgenic plants have higher APX activity in leaves under zinc stress
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no ascorbate peroxidase activity is present in mature seeds but activity is detected after 24 h post imbibition and increases 14fold up to 144 h post imbibition
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
agriculture
-
the results suggest that HvAPX1 plays an important role in zinc and cadmium tolerance, and might be a candidate gene for developing high-biomass tolerant plants for phytoremediation of zinc- and cadmium-polluted environments
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Xu, W.; Shi, W.; Liu, F.; Ueda, A.; Takabe, T.
Enhanced zinc and cadmium tolerance and accumulation in transgenic Arabidopsis plants constitutively overexpressing a barley gene (HvAPX1) that encodes a peroxisomal ascorbate peroxidase
Botany
86
567-575
2008
Hordeum vulgare
-
Manually annotated by BRENDA team
Bonsager, B.C.; Shahpiri, A.; Finnie, C.; Svensson, B.
Proteomic and activity profiles of ascorbate-glutathione cycle enzymes in germinating barley embryo
Phytochemistry
71
1650-1656
2010
Hordeum vulgare (O23983), Hordeum vulgare (Q945R5), Hordeum vulgare
Manually annotated by BRENDA team