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Information on EC 1.10.3.16 - dihydrophenazinedicarboxylate synthase and Organism(s) Pseudomonas fluorescens and UniProt Accession Q51793

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IUBMB Comments
Requires FMN. The enzyme, isolated from the bacteria Pseudomonas fluorescens 2-79 and Burkholderia lata 383, is involved in biosynthesis of the reduced forms of phenazine, phenazine-1-carboxylate, and phenazine-1,6-dicarboxylate, where it catalyses multiple reactions.
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This record set is specific for:
Pseudomonas fluorescens
UNIPROT: Q51793
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The taxonomic range for the selected organisms is: Pseudomonas fluorescens
The enzyme appears in selected viruses and cellular organisms
Synonyms
Bcep18194_B1572, phzG, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
phzG
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SYSTEMATIC NAME
IUBMB Comments
1,4,5a,6,9,10a-hexahydrophenazine-1,6-dicarboxylate:oxygen oxidoreductase
Requires FMN. The enzyme, isolated from the bacteria Pseudomonas fluorescens 2-79 and Burkholderia lata 383, is involved in biosynthesis of the reduced forms of phenazine, phenazine-1-carboxylate, and phenazine-1,6-dicarboxylate, where it catalyses multiple reactions.
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
(1R,10aS)-1,4,10,10a-tetrahydrophenazine-1,6-dicarboxylic acid + O2
(5aS)-5,5a-dihydrophenazine-1,6-dicarboxylic acid + H2O2
show the reaction diagram
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?
(1R,10aS)-1,4,10,10a-tetrahydrophenazine-1-carboxylic acid + O2
(10aS)-10,10a-dihydrophenazine-1-carboxylic acid + H2O2
show the reaction diagram
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-
?
(1R,5aS,6R)-1,4,5,5a,6,9-hexahydrophenazine-1,6-dicarboxylic acid + O2
(1R,10aS)-1,4,10,10a-tetrahydrophenazine-1,6-dicarboxylic acid + H2O2
show the reaction diagram
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?
additional information
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
UNIPROT
ENTRY NAME
ORGANISM
NO. OF AA
NO. OF TRANSM. HELICES
MOLECULAR WEIGHT[Da]
SOURCE
SEQUENCE
LOCALIZATION PREDICTION?
PHZG_PSEFL
222
0
24935
Swiss-Prot
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
complex with excess FMN and with the phenazine-biosynthesis intermediates hexahydrophenazine-1,6-dicarboxylate and tetrahydrophenazine-1-carboxylate generated in situ
structure of PhzG solved in an unliganded state at 1.9 and A resolution
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Parsons, J.F.; Calabrese, K.; Eisenstein, E.; Ladner, J.E.
Structure of the phenazine biosynthesis enzyme PhzG
Acta Crystallogr. Sect. D
60
2110-2113
2004
Pseudomonas aeruginosa (O69755), Pseudomonas fluorescens (Q51793)
Manually annotated by BRENDA team
Xu, N.; Ahuja, E.G.; Janning, P.; Mavrodi, D.V.; Thomashow, L.S.; Blankenfeldt, W.
Trapped intermediates in crystals of the FMN-dependent oxidase PhzG provide insight into the final steps of phenazine biosynthesis
Acta Crystallogr. Sect. D
69
1403-1413
2013
Burkholderia lata (Q396C5), Burkholderia lata ATCC 17760 (Q396C5), Pseudomonas fluorescens (Q51793), Pseudomonas fluorescens 2-79 (Q51793)
Manually annotated by BRENDA team