Information on EC 1.1.99.B9 - (S)-2-hydroxycarboxylate dehydrogenase

additional information

Sinorhizobium sp. ZJB1101

additional information

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COFACTOR

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

FMN

740300, 740783

additional information

does not require NAD(P)+ as cofactors to catalyze the oxidation reaction

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

81.2

(2S)-(2-chlorophenyl)(hydroxy)acetic acid

pH 8.0, 30°C

11.5

(2S)-(2-fluorophenyl)(hydroxy)acetic acid

pH 8.0, 30°C

4.9

(2S)-(3-chlorophenyl)(hydroxy)acetic acid

pH 8.0, 30°C

3.6

(2S)-(4-bromophenyl)(hydroxy)acetic acid

pH 8.0, 30°C

3.7

(2S)-(4-chlorophenyl)(hydroxy)acetic acid

pH 8.0, 30°C

66.5

(2S)-(4-fluorophenyl)(hydroxy)acetic acid

pH 8.0, 30°C

15.2

(2S)-hydroxy(2,4-difluorophenyl)acetic acid

pH 8.0, 30°C

8.6

(2S)-hydroxy(3,5-difluorophenyl)acetic acid

pH 8.0, 30°C

73.5

(2S)-hydroxy(4-hydroxyphenyl)acetic acid

pH 8.0, 30°C

65.6

(2S)-hydroxy(4-methylphenyl)acetic acid

pH 8.0, 30°C

64.9

(2S)-hydroxy(phenyl)acetic acid

pH 8.0, 30°C

68.5

(2S)-hydroxy-3-phenylpropanoic acid

pH 8.0, 30°C

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only manually annotated data include textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks include all textmining data from AMENDAenzyme occurrence in organism, localization and source tissue with reliability ranks + FRENDAco-occurrence of enzyme names and organism names (less precise)

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ORGANISM

COMMENTARY

LITERATURE

UNIPROT

SEQUENCE DB

SOURCE

Pseudomonas aeruginosa CCTCC M2011394

740300, 740783

brenda

740300

brenda

brenda

Sinorhizobium sp. ZJB1101

brenda

brenda

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EXPRESSION

ORGANISM

UNIPROT

LITERATURE

presence of 2-chloromandelic acid induces enzyme synthesis efficiently

2 entries

presence of 2-chloromandelic acid induces enzyme synthesis efficiently

presence of 2-chloromandelic acid induces enzyme synthesis efficiently

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APPLICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

synthesis

4 entries

synthesis

coupling of asymmetric oxidation of racemic 2-hydroxyacids with the opposite stereoselective reduction of 2-ketoacids for the stereoselective oxidoreductive deracemization to (R)-2-hydroxyacids, using a one-pot biocatalysis by resting cells of Saccharomyces cerevisiae strain ZJB5074 and Pseudomonas aeruginosa CCTCC M 2011394. Substituted racemic hydroxy(phenyl)acetic acids are converted to (R)-2-hydroxy-2-phenyl acetic acids with 55-98% conversion rate and 99.9% enantiomeric excess

740300

synthesis

enzyme can be used for the concurrent obtaining of aromatic (R)-2-hydroxyacids and aromatic 2-ketoacids by oxidation of aromatic 2-hydroxyacids in one-step biotransformation

Pseudomonas aeruginosa CCTCC M2011394

synthesis

synthesis

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enzyme can be used for the concurrent obtaining of aromatic (R)-2-hydroxyacids and aromatic 2-ketoacids by oxidation of aromatic 2-hydroxyacids in one-step biotransformation

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REF.

AUTHORS

TITLE

JOURNAL

VOL.

PAGES

YEAR

ORGANISM (UNIPROT)

PUBMED ID

SOURCE

Xue, Y.; Zhang, Y.; Wang, W.; Wang, Y.; Liu, Z.; Zou, S.; Zheng, Y.; Shen, Y.

Highly enantioselective oxidation of alpha-hydroxyacids bearing a substituent with an aryl group: Co-production of optically active alpha-hydroxyacids and alpha-ketoacids

Biores. Technol.

132

391-394

2013

Sinorhizobium sp., Sinorhizobium sp. ZJB1101

23273376

brenda

740300

Xue, Y.; Zheng, Y.; Zhang, Y.; Sun, J.; Liu, Z.; Shen, Y.

One-pot, single-step deracemization of 2-hydroxyacids by tandem biocatalytic oxidation and reduction

Chem. Commun. (Camb.)

10706-10708

2013

Pseudomonas aeruginosa, Pseudomonas aeruginosa CCTCC M2011394

24100587

brenda