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IUBMB Comments The enzyme, characterized in the yeast Saccharomyces cerevisiae , also functions as EC 1.1.2.4 , D -lactate dehydrogenase (cytochrome), and is active with oxaloacetate as electron acceptor forming (R )-malate.
Synonyms DLD3 ,
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D-2-hydroxyglutarate-pyruvate transhydrogenase
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DLD3
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(R)-2-hydroxyglutarate + pyruvate = 2-oxoglutarate + (R)-lactate
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(R)-2-hydroxyglutarate:pyruvate oxidoreductase [(R)-lactate-forming]
The enzyme, characterized in the yeast Saccharomyces cerevisiae, also functions as EC 1.1.2.4, D-lactate dehydrogenase (cytochrome), and is active with oxaloacetate as electron acceptor forming (R)-malate.
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2-oxoglutarate + D-lactate
D-2-hydroxyglutarate + pyruvate
Substrates: -
r
D-2-hydroxyglutarate + oxaloacetate
2-oxoglutarate + malate
Substrates: -
r
D-2-hydroxyglutarate + pyruvate
2-oxoglutarate + D-lactate
Substrates: -
r
additional information
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Substrates: no substrate: L-lactate. The enzyme displays dehydrogenase activity with artificial acceptor dichlorophenolindophenol
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FAD
enzyme is a FAD-dependent transhydrogenase using pyruvate as a hydrogen acceptor
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Co2+
0.005 mM, about 3fold stimulation
Zn2+
0.005 mM, about 3fold stimulation
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0.111
D-2-hydroxyglutarate
pH 9.5, 30°C
0.45
pyruvate
pH 9.5, 30°C
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4
D-2-hydroxyglutarate
pH 9.5, 30°C
4.9
pyruvate
pH 9.5, 30°C
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36
D-2-hydroxyglutarate
pH 9.5, 30°C
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UniProt
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Highest Expressing Human Cell Lines
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Cell Line Links
Gene Links
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physiological function
a mutant strain lacking cytosolic transhydrogenase Dld3 activity accumulates millimolar levels of D-2-hydroxyglutarate
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DLD2_YEAST
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
530
0
59268
Swiss-Prot
Mitochondrion (Reliability: 2 )
DLD3_YEAST
Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
496
0
55225
Swiss-Prot
other Location (Reliability: 3 )
A0A8J8WJ64_9EURO
543
0
60017
TrEMBL
Mitochondrion (Reliability: 1 )
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recombinant protein. Purified fractions show a yellow color
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Becker-Kettern, J.; Paczia, N.; Conrotte, J.F.; Kay, D.P.; Guignard, C.; Jung, P.P.; Linster, C.L.
Saccharomyces cerevisiae forms D-2-hydroxyglutarate and couples its degradation to D-lactate formation via a cytosolic transhydrogenase
J. Biol. Chem.
291
6036-6058
2016
Saccharomyces cerevisiae (P39976)
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