Information on EC 1.1.99.4 - dehydrogluconate dehydrogenase

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The enzyme appears in viruses and cellular organisms

EC NUMBER
COMMENTARY hide
1.1.99.4
-
RECOMMENDED NAME
GeneOntology No.
dehydrogluconate dehydrogenase
REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
2-dehydro-D-gluconate + acceptor = 2,5-didehydro-D-gluconate + reduced acceptor
show the reaction diagram
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
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redox reaction
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-
-
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reduction
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-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
L-ascorbate biosynthesis VI (engineered pathway)
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ketogluconate metabolism
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SYSTEMATIC NAME
IUBMB Comments
2-dehydro-D-gluconate:acceptor 2-oxidoreductase
A flavoprotein.
CAS REGISTRY NUMBER
COMMENTARY hide
9028-82-4
-
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
IFO 3245
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-
Manually annotated by BRENDA team
cytochrome c subunit and large subunit and small subunit
A0A0B6VQG4 and A0A0B6VN56 and A0A0B6VKP6
UniProt
Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
-
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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Manually annotated by BRENDA team
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-
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Manually annotated by BRENDA team
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-dehydro-D-gluconate + 2,6-dichlorophenolindophenol
2,5-didehydro-D-gluconate + ?
show the reaction diagram
2-dehydro-D-gluconate + acceptor
?
show the reaction diagram
2-dehydro-D-gluconate + ferricyanide
2,5-didehydro-D-gluconate + ferrocyanide
show the reaction diagram
2-dehydro-D-gluconate + methylene blue
2,5-didehydro-D-gluconate + ?
show the reaction diagram
-
methylene blue is not as effective as 2,6-dichlorophenolindophenol
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-
?
2-dehydro-D-gluconate + nitro blue tetrazolium
2,5-didehydro-D-gluconate + ?
show the reaction diagram
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-
-
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?
2-dehydro-D-gluconate + phenazine methosulfate
2,5-didehydro-D-gluconate + ?
show the reaction diagram
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-
-
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?
2-dehydro-D-gluconate + pyocyanine
2,5-didehydro-D-gluconate + ?
show the reaction diagram
-
pyocyanine is not as effective as 2,6-dichlorophenolindophenol
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?
additional information
?
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
2-dehydro-D-gluconate + acceptor
?
show the reaction diagram
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
cytochrome
-
flavin
INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
-
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
50
2-dehydro-D-gluconate
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reaction with ferricyanide
SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25000
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1 * 61000, flavoprotein + 1 * 47000, cytochrome component, + 1 * 25000, function unclear, SDS-PAGE
47000
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1 * 61000, flavoprotein + 1 * 47000, cytochrome component, + 1 * 25000, function unclear, SDS-PAGE
61000
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1 * 61000, flavoprotein + 1 * 47000, cytochrome component, + 1 * 25000, function unclear, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
trimer
pH STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8
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stable from pH 5.0 to pH 8.0
389926, 389927
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
Triton X-100 stabilizes
-
Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expressed in Gluconobacter japonicas strain NBRC3271
A0A0B6VQG4 and A0A0B6VN56 and A0A0B6VKP6
APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
analysis
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enzymic microdetermination of 2-keto-D-gluconate