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Information on EC 1.1.99.4 - dehydrogluconate dehydrogenase for references in articles please use BRENDA:EC1.1.99.4
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EC Tree
The enzyme appears in viruses and cellular organisms
Synonyms
2kgdh, 2-keto-d-gluconate dehydrogenase, 2-keto-gluconate dehydrogenase,
more
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2-keto-D-gluconate dehydrogenase
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2-keto-gluconate dehydrogenase
2-oxogluconate dehydrogenase
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alpha-ketogluconate dehydrogenase
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dehydrogenase, ketogluconate
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ketogluconate dehydrogenase
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2-keto-gluconate dehydrogenase
A0A0B6VQG4; A0A0B6VN56; A0A0B6VKP6
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2-keto-gluconate dehydrogenase
A0A0B6VQG4; A0A0B6VN56; A0A0B6VKP6
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2KGDH
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2KGDH
A0A0B6VQG4; A0A0B6VN56; A0A0B6VKP6
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2KGDH
A0A0B6VQG4; A0A0B6VN56; A0A0B6VKP6
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2-dehydro-D-gluconate + acceptor = 2,5-didehydro-D-gluconate + reduced acceptor
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2-dehydro-D-gluconate:acceptor 2-oxidoreductase
A flavoprotein.
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2-dehydro-D-gluconate + 2,6-dichlorophenolindophenol
2,5-didehydro-D-gluconate + ?
2-dehydro-D-gluconate + acceptor
?
2-dehydro-D-gluconate + ferricyanide
2,5-didehydro-D-gluconate + ferrocyanide
2-dehydro-D-gluconate + methylene blue
2,5-didehydro-D-gluconate + ?
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methylene blue is not as effective as 2,6-dichlorophenolindophenol
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-
?
2-dehydro-D-gluconate + nitro blue tetrazolium
2,5-didehydro-D-gluconate + ?
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?
2-dehydro-D-gluconate + phenazine methosulfate
2,5-didehydro-D-gluconate + ?
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-
-
?
2-dehydro-D-gluconate + pyocyanine
2,5-didehydro-D-gluconate + ?
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pyocyanine is not as effective as 2,6-dichlorophenolindophenol
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?
additional information
?
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2-dehydro-D-gluconate + 2,6-dichlorophenolindophenol
2,5-didehydro-D-gluconate + ?
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?
2-dehydro-D-gluconate + 2,6-dichlorophenolindophenol
2,5-didehydro-D-gluconate + ?
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?
2-dehydro-D-gluconate + 2,6-dichlorophenolindophenol
2,5-didehydro-D-gluconate + ?
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?
2-dehydro-D-gluconate + 2,6-dichlorophenolindophenol
2,5-didehydro-D-gluconate + ?
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?
2-dehydro-D-gluconate + acceptor
?
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enzyme of the D-glucose oxidation pathway
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?
2-dehydro-D-gluconate + acceptor
?
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enzyme is the primary dehydrogenase of the 2-keto-D-gluconate oxidizing system
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?
2-dehydro-D-gluconate + acceptor
?
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enzyme of the D-glucose oxidation pathway
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?
2-dehydro-D-gluconate + acceptor
?
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enzyme of the D-glucose oxidation pathway
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?
2-dehydro-D-gluconate + acceptor
?
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enzyme of the D-glucose oxidation pathway
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?
2-dehydro-D-gluconate + acceptor
?
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enzyme of the D-glucose oxidation pathway
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-
?
2-dehydro-D-gluconate + acceptor
?
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enzyme of the D-glucose oxidation pathway
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-
?
2-dehydro-D-gluconate + acceptor
?
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enzyme of the D-glucose oxidation pathway
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-
?
2-dehydro-D-gluconate + acceptor
?
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enzyme of the D-glucose oxidation pathway
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-
?
2-dehydro-D-gluconate + ferricyanide
2,5-didehydro-D-gluconate + ferrocyanide
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?
2-dehydro-D-gluconate + ferricyanide
2,5-didehydro-D-gluconate + ferrocyanide
A0A0B6VQG4; A0A0B6VN56; A0A0B6VKP6
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-
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?
2-dehydro-D-gluconate + ferricyanide
2,5-didehydro-D-gluconate + ferrocyanide
A0A0B6VQG4; A0A0B6VN56; A0A0B6VKP6
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-
-
?
2-dehydro-D-gluconate + ferricyanide
2,5-didehydro-D-gluconate + ferrocyanide
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?
additional information
?
-
A0A0B6VQG4; A0A0B6VN56; A0A0B6VKP6
the enzyme is completely inert toward 5-oxoglutarate, D-gluconate, D-glucose, D-sorbitol, L-lactate, L-malate, and D-fructose
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additional information
?
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the enzyme is completely inert toward 5-oxoglutarate, D-gluconate, D-glucose, D-sorbitol, L-lactate, L-malate, and D-fructose
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additional information
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A0A0B6VQG4; A0A0B6VN56; A0A0B6VKP6
the enzyme is completely inert toward 5-oxoglutarate, D-gluconate, D-glucose, D-sorbitol, L-lactate, L-malate, and D-fructose
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additional information
?
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the enzyme is completely inert toward 5-oxoglutarate, D-gluconate, D-glucose, D-sorbitol, L-lactate, L-malate, and D-fructose
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2-dehydro-D-gluconate + acceptor
?
2-dehydro-D-gluconate + acceptor
?
-
enzyme of the D-glucose oxidation pathway
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-
?
2-dehydro-D-gluconate + acceptor
?
-
enzyme is the primary dehydrogenase of the 2-keto-D-gluconate oxidizing system
-
-
?
2-dehydro-D-gluconate + acceptor
?
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enzyme of the D-glucose oxidation pathway
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-
?
2-dehydro-D-gluconate + acceptor
?
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enzyme of the D-glucose oxidation pathway
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?
2-dehydro-D-gluconate + acceptor
?
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enzyme of the D-glucose oxidation pathway
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-
?
2-dehydro-D-gluconate + acceptor
?
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enzyme of the D-glucose oxidation pathway
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-
?
2-dehydro-D-gluconate + acceptor
?
-
enzyme of the D-glucose oxidation pathway
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-
?
2-dehydro-D-gluconate + acceptor
?
-
enzyme of the D-glucose oxidation pathway
-
-
?
2-dehydro-D-gluconate + acceptor
?
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enzyme of the D-glucose oxidation pathway
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-
?
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cytochrome
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contains a cytochrome component
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cytochrome
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contains 0.91 mol of tightly bound c-type cytochrome per mol of enzyme
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flavin
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contains 8alpha-(N3-histidyl) riboflavin in the dinucleotide form; flavoprotein
flavin
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contains 0.44 mol of covalently bound flavin per mol of enzyme; flavoprotein
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CN-
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1 mM, 40% inhibition
additional information
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not: sulfhydryl reagents
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additional information
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metal chelators; not: sulfhydryl reagents
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50
2-dehydro-D-gluconate
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reaction with ferricyanide
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brenda
IFO 3245
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brenda
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brenda
cytochrome c subunit and large subunit and small subunit
A0A0B6VQG4; A0A0B6VN56; A0A0B6VKP6
UniProt
brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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brenda
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-
brenda
cytochrome c subunit and large subunit and small subunit
A0A0B6VQG4; A0A0B6VN56; A0A0B6VKP6
UniProt
brenda
i.e. Acetobacter melanogenus
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brenda
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outer surface of cytoplasmic membrane
brenda
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brenda
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brenda
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brenda
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A0A0T9NEF0_9GAMM
169
0
18445
TrEMBL
A0A0U1KI37_YERMO
544
0
60068
TrEMBL
A0A0T9KJ06_9GAMM
534
0
59215
TrEMBL
A0A0A1HRU1_9PSED
184
0
19845
TrEMBL
A0A0A1HSK0_9PSED
393
1
42585
TrEMBL
I0K9K1_9BACT
572
0
64048
TrEMBL
A0A085JPG3_9GAMM
546
0
60322
TrEMBL
A0A085JEP8_9GAMM
195
0
21456
TrEMBL
A0A0H3NXJ5_YERE1
Yersinia enterocolitica subsp. palearctica serotype O:3 (strain DSM 13030 / CIP 106945 / Y11)
422
1
46398
TrEMBL
A0A0U1KID2_YERMO
169
0
18356
TrEMBL
A0A0A1HRN6_9PSED
544
0
59764
TrEMBL
A0A177GGB7_9PROT
184
0
19610
TrEMBL
A0A085GM69_9GAMM
535
0
59796
TrEMBL
A0A085JPG2_9GAMM
177
0
19364
TrEMBL
A0A0T9TMZ2_YERKR
169
1
18392
TrEMBL
M4URU0_RALSL
539
1
59693
TrEMBL
A0A087PX77_9PROT
545
0
60997
TrEMBL
A0A094YI55_9PROT
206
0
22487
TrEMBL
A3RYA9_RALSL
431
0
46336
TrEMBL
A3RYA8_RALSL
540
1
59793
TrEMBL
A0A0A1FKE9_9BURK
533
0
58765
TrEMBL
A0A0T9UEV2_YEREN
169
1
18375
TrEMBL
A0A0E1NK72_YEREN
539
0
59619
TrEMBL
A0A0T7NQP9_YEREN
544
0
60046
TrEMBL
A0A0T9NF95_9GAMM
544
1
59941
TrEMBL
A0A0A0BIW3_9GAMM
558
0
60581
TrEMBL
A0A0T9NEP0_YERKR
169
0
18525
TrEMBL
A0A0T9S541_YEREN
539
0
59559
TrEMBL
A0A380PSI5_YERFR
169
0
18376
TrEMBL
A0A177GDU6_9PROT
206
0
22709
TrEMBL
A0A0T9NEH3_YERKR
539
0
59577
TrEMBL
A0A094ZEG1_9PROT
545
0
60592
TrEMBL
A0A1B7KFJ3_9GAMM
449
1
47439
TrEMBL
A0A085JD98_9GAMM
189
0
20387
TrEMBL
A0A0L0MEH6_9BURK
188
1
20118
TrEMBL
A0A0U1HBP2_YEREN
169
1
18511
TrEMBL
A0A060QCX4_9PROT
204
0
22041
TrEMBL
A0A0H3C822_CAUVN
Caulobacter vibrioides (strain NA1000 / CB15N)
579
0
64397
TrEMBL
A0A0T9UFU5_YERFR
539
0
59554
TrEMBL
A0A380PSI1_YERFR
539
0
59515
TrEMBL
A0A0T9UGM8_YERFR
169
0
18373
TrEMBL
A0A0T9TUW5_YERIN
539
0
59565
TrEMBL
A0A060QDI6_9PROT
485
1
53066
TrEMBL
A0A0T9SY60_YERAL
169
0
18452
TrEMBL
A0A0T9L9D9_YERKR
539
0
59588
TrEMBL
M4UCD4_RALSL
429
0
46451
TrEMBL
W8FL89_AGRT4
486
1
52208
TrEMBL
A0A0T9TUY4_YERIN
169
1
18526
TrEMBL
W0HNP6_9GAMM
447
0
47629
TrEMBL
A0A085G9H8_9GAMM
469
1
50255
TrEMBL
A0A085JDA0_9GAMM
474
1
51085
TrEMBL
A0A0T9S523_YEREN
169
1
18525
TrEMBL
A0A060QC92_9PROT
536
0
59309
TrEMBL
A0A0H3NSN4_YERE1
Yersinia enterocolitica subsp. palearctica serotype O:3 (strain DSM 13030 / CIP 106945 / Y11)
531
0
58797
TrEMBL
G0AFT0_COLFT
Collimonas fungivorans (strain Ter331)
206
0
21996
TrEMBL
A0A0T9SX35_YERAL
539
0
59454
TrEMBL
A0A143GBU4_9PSED
529
0
58020
TrEMBL
A0A143GC18_9PSED
480
1
51199
TrEMBL
A0A094YG46_9PROT
435
0
46238
TrEMBL
A0A085JPG1_9GAMM
450
1
48129
TrEMBL
A0A085JEP6_9GAMM
543
0
59808
TrEMBL
A0A0K9K153_9BURK
120
0
12212
TrEMBL
A0A0A1FH91_9BURK
204
0
21808
TrEMBL
A0A0T7NQS0_YEREN
169
1
18444
TrEMBL
A0A0U1HBM7_YEREN
102
0
11094
TrEMBL
A0A177GDU2_9PROT
159
1
16329
TrEMBL
M4UAQ1_RALSL
142
0
14840
TrEMBL
A0A0U1I9U2_YERMO
169
1
18375
TrEMBL
A0A0U1I9Z1_YERMO
544
0
60073
TrEMBL
A0A085GM68_9GAMM
169
0
18322
TrEMBL
A0A0U1HBI0_YEREN
539
0
59581
TrEMBL
A0A0H3P0D0_YERE1
Yersinia enterocolitica subsp. palearctica serotype O:3 (strain DSM 13030 / CIP 106945 / Y11)
169
1
18513
TrEMBL
A0A177GDT2_9PROT
211
0
22706
TrEMBL
A0A085G9H7_9GAMM
551
0
60217
TrEMBL
A0A085G9H6_9GAMM
205
0
21772
TrEMBL
A0A0T9UEZ9_YEREN
544
0
60068
TrEMBL
A0A0T9P006_YERKR
539
0
59544
TrEMBL
A0A0A1F6U0_9BURK
532
0
58072
TrEMBL
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25000
-
1 * 61000, flavoprotein + 1 * 47000, cytochrome component, + 1 * 25000, function unclear, SDS-PAGE
47000
-
1 * 61000, flavoprotein + 1 * 47000, cytochrome component, + 1 * 25000, function unclear, SDS-PAGE
61000
-
1 * 61000, flavoprotein + 1 * 47000, cytochrome component, + 1 * 25000, function unclear, SDS-PAGE
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trimer
-
1 * 61000, flavoprotein + 1 * 47000, cytochrome component, + 1 * 25000, function unclear, SDS-PAGE
trimer
A0A0B6VQG4; A0A0B6VN56; A0A0B6VKP6
a small subunit, a large subunit, and a cytochrome c subunit
trimer
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a small subunit, a large subunit, and a cytochrome c subunit
-
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5 - 8
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stable from pH 5.0 to pH 8.0
389926, 389927
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Triton X-100 stabilizes
-
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expressed in Gluconobacter japonicas strain NBRC3271
A0A0B6VQG4; A0A0B6VN56; A0A0B6VKP6
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analysis
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enzymic microdetermination of 2-keto-D-gluconate
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Datta, A.G.; Katznelson, H.
The oxidation of 2-ketogluconate by a partially purified enzyme from Acetobacter melanogenum
Arch. Biochem. Biophys.
65
576-578
1956
Gluconobacter oxydans
brenda
Bouvet, O.M.M.; Lenormand, P.; Grimont, P.A.D.
Taxonomic diversity of the D-glucose oxidation pathway in the Enterobacteriaceae
Int. J. Syst. Bacteriol.
39
61-67
1989
Ewingella americana, Pantoea cypripedii, Rahnella aquatilis, Serratia grimesii, Serratia liquefaciens, Serratia marcescens, Shimwellia blattae, Tatumella ptyseos
-
brenda
McIntire, W.; Singer, T.P.; Ameyama, M.; Adachi, O.; Matsushita, K.; Shinagawa, E.
Identification of the covalently bound flavins of D-gluconate dehydrogenases from Pseudomonas aeruginosa and Pseudomonas fluorescens and of 2-keto-D-gluconate dehydrogenase from Gluconobacter melanogenus
Biochem. J.
231
651-654
1985
Gluconobacter oxydans
brenda
Ameyama, M.
Enzymic microdetermination of D-glucose, D-fructase, D-gluconate, 2-keto-D-gluconate, aldehyde, and alcohol with membrane-bound dehydrogenase
Methods Enzymol.
89
20-29
1982
Gluconobacter sp.
-
brenda
Shinagawa, E.; Ameyama, M.
2-Keto-D-gluconate dehydrogenase from Gluconobacter melanogenum, membrane-bound
Methods Enzymol.
89
194-198
1982
Gluconobacter oxydans
-
brenda
Shinagawa, E.; Matsushita, K.; Adachi, O.; Ameyama, M.
Purification and characterization of 2-keto-D-gluconate dehydrogenase from Gluconobacter melanogenus
Agric. Biol. Chem.
45
1079-1085
1981
Gluconobacter oxydans
-
brenda
Shinagawa, E.; Chiyonobu, T.; Adachi, O.; Ameyama, M.
Distribution and solubilization of particulate gluconate dehydrogenase in acetic acid bacteria
Agric. Biol. Chem.
40
475-483
1976
Frateuria aurantia, Gluconacetobacter liquefaciens, Gluconobacter oxydans, Gluconobacter sphaericus
-
brenda
Kataoka, N.; Matsutani, M.; Yakushi, T.; Matsushita, K.
Efficient production of 2,5-diketo-D-gluconate via heterologous expression of 2-ketogluconate dehydrogenase in Gluconobacter japonicus
Appl. Environ. Microbiol.
81
3552-3560
2015
Gluconobacter oxydans (A0A0B6VQG4 and A0A0B6VN56 and A0A0B6VKP6), Gluconobacter oxydans, Gluconobacter oxydans NBRC3293 (A0A0B6VQG4 and A0A0B6VN56 and A0A0B6VKP6), Gluconobacter oxydans NBRC3293
brenda
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