Any feedback?
Information on EC 1.1.3.8 - L-gulonolactone oxidase
for references in articles please use BRENDA:EC1.1.3.8Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
1.1.3.8 L-gulonolactone oxidaseIUBMB Comments
A microsomal flavoprotein (FAD). The product spontaneously isomerizes to L-ascorbate. While most higher animals can synthesize asborbic acid, primates and guinea pigs cannot .
Specify your search results
Word Map
- 1.1.3.8
-
l-ascorbic
- scurvy
-
scurvy-prone
-
levoglucosenone
-
scorbutic
- dibenzothiophene
-
shionogi
-
lemongrass
- l-galactono-1,4-lactone
- nutrition
- medicine
- molecular biology
The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea
Synonyms
lgo, l-gulonolactone oxidase, l-gulono-gamma-lactone oxidase, gulonolactone oxidase, l-gulono-1,4-lactone oxidase, gullo, gloase, atgullo5, atgullo2, l-gulono-1,4-lactone dehydrogenase, 
more
topPlease wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
GLO
GLOase
GulLO5
GULO
gulonolactone oxidase
L-gulono-1,4-lactone dehydrogenase
L-gulono-1,4-lactone oxidase
L-gulono-gamma-lactone oxidase
L-gulono-gamma-lactone: O2 oxidoreductase
-
-
-
-
L-gulono-gamma-lactone:oxidoreductase
-
-
-
-
LGO
-
-
-
-
L-gulono-gamma-lactone oxidase
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2
product isomerizes spontaneously to L-ascorbate
-
L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2
product isomerizes spontaneously to L-ascorbate
-
L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2
product isomerizes spontaneously to L-ascorbate
-
L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2
product isomerizes spontaneously to L-ascorbate
-
L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2
product isomerizes spontaneously to L-ascorbate
-
L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2
product isomerizes spontaneously to L-ascorbate
-
L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2
product isomerizes spontaneously to L-ascorbate
-
L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2
product isomerizes spontaneously to L-ascorbate
-
L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2
product isomerizes spontaneously to L-ascorbate
-
L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2
product isomerizes spontaneously to L-ascorbate
-
L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2
product isomerizes spontaneously to L-ascorbate
-
L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2
product isomerizes spontaneously to L-ascorbate
-
L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2
product isomerizes spontaneously to L-ascorbate
-
L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2
product isomerizes spontaneously to L-ascorbate
-
L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2
product isomerizes spontaneously to L-ascorbate
-
L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2
product isomerizes spontaneously to L-ascorbate
-
L-gulono-1,4-lactone + O2 = L-xylo-hex-2-ulono-1,4-lactone + H2O2
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SYSTEMATIC NAME
IUBMB Comments
L-gulono-1,4-lactone:oxygen 3-oxidoreductase
A microsomal flavoprotein (FAD). The product spontaneously isomerizes to L-ascorbate. While most higher animals can synthesize asborbic acid, primates and guinea pigs cannot [3].
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
CAS REGISTRY NUMBER
COMMENTARY
9028-78-8
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
creatol + O2
methylguanidine + ?
-
creatone A and creatone B are intermediates
-
-
?
D-glucose + phenazine methosulfate + O2
D-glucosone + ?
-
23.4% as active as L-gulono-1,4-lactone
-
-
?
D-mannose + phenazine methosulfate + O2
?
-
6.4% as active as L-gulono-1,4-lactone
-
-
?
D-xylose + phenazine methosulfate + O2
D-xylosone + ?
-
6.4% as active as L-gulono-1,4-lactone
-
-
?
L-glucurono-1,4-lactone + phenazine methosulfate + O2
?
-
6.4% as active as L-gulono-1,4-lactone
-
-
?
L-gulono-1,4-lactone + cytochrome C
L-ascorbate + ?
-
-
-
?
L-gulono-1,4-lactone + O2
L-xylo-hex-2-ulono-1,4-lactone + H2O2
-
-
-
-
r
L-galactono-1,4-lactone + O2
L-ascorbic acid + H2O2
-
-
-
?
L-galactono-1,4-lactone + O2
L-ascorbic acid + H2O2
-
90% as active as L-gulono-1,4-lactone
-
-
?
L-galactono-1,4-lactone + O2
L-ascorbic acid + H2O2
-
2% as active as L-gulono-1,4-lactone
-
-
?
L-galactono-1,4-lactone + O2
L-ascorbic acid + H2O2
-
-
-
-
?
L-glucono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
-
-
r
L-gulono-1,4-lactone + 2,6-dichlorophenolindophenol + O2
L-ascorbic acid + ?
-
enzyme is a dehydrogenase, not an oxidase
-
-
?
L-gulono-1,4-lactone + 2,6-dichlorophenolindophenol + O2
L-ascorbic acid + ?
-
-
-
-
?
L-gulono-1,4-lactone + O2
L-ascorbate + H2O2
-
-
-
?
L-gulono-1,4-lactone + O2
L-ascorbate + H2O2
during the catalytic reaction by Gulo, flavin accepts 2 electrons from the substrate l-gulono-gamma-lactone, thus undergoing a reduction, followed by oxidation through molecular oxygen, with the reaction presumably involving the ALO domain. Through this process, 2-oxo-l-gulono-gamma-lactone and hydrogen peroxide are produced, and L-ascorbic acid is formed by nonenzymatic isomerisation of the former product
-
-
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
-
-
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
the enzyme has a configurational specificity for the hydroxyl group at C-2
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
the enzyme has a configurational specificity for the hydroxyl group at C(2)
-
-
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
-
-
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
-
-
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
-
-
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
636305, 636306, 636307, 636309, 636310, 636311, 636315, 636317, 636319, 636320, 636321, 636322, 636323, 636324, 636325
-
-
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
the enzyme has a configurational specificity for the hydroxyl group at C-2
-
-
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
the enzyme has a configurational specificity for the hydroxyl group at C(2)
-
-
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
key enzyme for L-ascorbic acid biosynthesis
-
-
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
product isomerizes spontaneously to L-ascorbate
?
L-gulono-1,4-lactone + phenazine methosulfate + O2
L-ascorbic acid + ?
-
enzyme is a dehydrogenase, not an oxidase
-
-
?
L-gulono-1,4-lactone + phenazine methosulfate + O2
L-ascorbic acid + ?
-
-
-
-
?
L-gulono-1,4-lactone + phenazine methosulfate + O2
L-ascorbic acid + ?
-
reaction also takes place under anaerobic conditions
-
-
?
additional information
?
-
the enzyme catalyzes the reaction of L-gulonolactone oxidase, EC 1.1.3.8, producting L-ascorbate, but has sequence similarities to D-arabinono-1,4-lactone oxidase, EC 1.1.3.37
-
-
?
additional information
?
-
the enzyme catalyzes the reaction of L-gulonolactone oxidase, EC 1.1.3.8, producting L-ascorbate, but has sequence similarities to D-arabinono-1,4-lactone oxidase, EC 1.1.3.37
-
-
?
additional information
?
-
the enzyme catalyzes the reaction of L-gulonolactone oxidase, EC 1.1.3.8, producting L-ascorbate, but has sequence similarities to D-arabinono-1,4-lactone oxidase, EC 1.1.3.37
-
-
?
additional information
?
-
no dehydrogenase or oxidase activity with L-galactono-1,4-lactone, D-galactono-1,4-lactone, and D-gluconic acid-gamma-lactone. No oxidase activity with L-gulono-1,4-lactone
-
-
-
additional information
?
-
no dehydrogenase or oxidase activity with L-galactono-1,4-lactone, D-galactono-1,4-lactone, and D-gluconic acid-gamma-lactone. No oxidase activity with L-gulono-1,4-lactone
-
-
-
additional information
?
-
no substrates: raffinose, mannose, sucrose, arabinose, and ascorbate
-
-
-
additional information
?
-
-
no substrates: raffinose, mannose, sucrose, arabinose, and ascorbate
-
-
-
additional information
?
-
examination of metabolic role of vitamin C synthesis in cells or animals that, during evolution, have lost the ability to make ascorbic acid
-
-
?
additional information
?
-
-
examination of metabolic role of vitamin C synthesis in cells or animals that, during evolution, have lost the ability to make ascorbic acid
-
-
?
additional information
?
-
-
presence of a alternative pathway for vitamin C production in plants which can circumvent steps of the D-mannose pathway
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
L-gulono-1,4-lactone + O2
L-ascorbate + H2O2
-
-
-
?
L-gulono-1,4-lactone + O2
L-ascorbate + H2O2
during the catalytic reaction by Gulo, flavin accepts 2 electrons from the substrate l-gulono-gamma-lactone, thus undergoing a reduction, followed by oxidation through molecular oxygen, with the reaction presumably involving the ALO domain. Through this process, 2-oxo-l-gulono-gamma-lactone and hydrogen peroxide are produced, and L-ascorbic acid is formed by nonenzymatic isomerisation of the former product
-
-
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
-
-
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
the enzyme has a configurational specificity for the hydroxyl group at C(2)
-
-
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
-
-
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
-
-
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
-
-
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
-
636305, 636306, 636307, 636309, 636310, 636311, 636315, 636317, 636319, 636320, 636321, 636322, 636323, 636324, 636325
-
-
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
the enzyme has a configurational specificity for the hydroxyl group at C(2)
-
-
?
L-gulono-1,4-lactone + O2
L-xylo-hex-3-ulonolactone + H2O2
-
key enzyme for L-ascorbic acid biosynthesis
-
-
?
additional information
?
-
the enzyme catalyzes the reaction of L-gulonolactone oxidase, EC 1.1.3.8, producting L-ascorbate, but has sequence similarities to D-arabinono-1,4-lactone oxidase, EC 1.1.3.37
-
-
?
additional information
?
-
the enzyme catalyzes the reaction of L-gulonolactone oxidase, EC 1.1.3.8, producting L-ascorbate, but has sequence similarities to D-arabinono-1,4-lactone oxidase, EC 1.1.3.37
-
-
?
additional information
?
-
the enzyme catalyzes the reaction of L-gulonolactone oxidase, EC 1.1.3.8, producting L-ascorbate, but has sequence similarities to D-arabinono-1,4-lactone oxidase, EC 1.1.3.37
-
-
?
additional information
?
-
examination of metabolic role of vitamin C synthesis in cells or animals that, during evolution, have lost the ability to make ascorbic acid
-
-
?
additional information
?
-
-
examination of metabolic role of vitamin C synthesis in cells or animals that, during evolution, have lost the ability to make ascorbic acid
-
-
?
additional information
?
-
-
presence of a alternative pathway for vitamin C production in plants which can circumvent steps of the D-mannose pathway
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
FAD
-
8alpha-(N1-histidyl)riboflavin is the cofactor structure determined by high-voltage paper electrophoresis
FAD
-
8alpha-(N1-histidyl)riboflavin is the cofactor structure determined by high-voltage paper electrophoresis
FAD
-
8alpha-(N1-histidyl)riboflavin is the cofactor structure determined by high-voltage paper electrophoresis
FAD
the flavin adenine dinucleotide (FAD) binding domain comprises amino acids 21-155. FAD binds to the FAD binding domain via an 8-alpha-(N3-histidyl)-riboflavin linkage at the conserved histidine residue at position 54. During the catalytic reaction by Gulo, flavin accepts 2 electrons from the substrate l-gulono-gamma-lactone, thus undergoing a reduction, followed by oxidation through molecular oxygen, with the reaction presumably involving the ALO domain
flavin
-
covalently bound, flavoenzyme
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
Cu(NO3)2
-
0.19 mM concentration 12% inhibition, 0.38 mM concentration 76% inhibition and 0.89 mM concentration 100% inhibition
CuCl2 * 2H2O
-
0.19 mM concentration 16% inhibition, 0.38 mM concentration 92% inhibition and 0.89 mM concentration 100% inhibition
CuSO4
-
0.19 mM concentration 20% inhibition, 0.38 mM concentration 80% inhibition and 0.89 mM concentration 100% inhibition
L-gulono-1,4-lactone
enzyme activity is inhibited when L-gulono-1,4-lactone concentration is increased to 400 mM
Na2S
-
2 mM concentration 76% inhibition, 20 mM concentration 94% concentration
Na2SO3
-
5 mM concentration, 33% inhibition, 20 mM concentration 61% inhibition
NaCl
inhibitory (20-70% residual activity) between 5 and 300 mM
additional information
-
not inhibitory are: EDTA, sodium azide, monoiodoacetate, p-chloromercuribenzoate, NaHAsO4, sodium fluoride, KCN, hydroxyl ammonium sulfate, hydroxylamine, hydrazine monohydrate, hydrazinium dichloride, and 1,4-benzoquinone
-