Information on EC 1.1.1.81 - hydroxypyruvate reductase and Organism(s) Arabidopsis thaliana and UniProt Accession Q9LE33

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Arabidopsis thaliana
UNIPROT: Q9LE33
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The expected taxonomic range for this enzyme is: Eukaryota, Bacteria, Archaea


The taxonomic range for the selected organisms is: Arabidopsis thaliana

EC NUMBER
COMMENTARY hide
1.1.1.81
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RECOMMENDED NAME
GeneOntology No.
hydroxypyruvate reductase
REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
formaldehyde assimilation I (serine pathway)
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L-serine biosynthesis II
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serine metabolism
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Glycine, serine and threonine metabolism
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Glyoxylate and dicarboxylate metabolism
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Metabolic pathways
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Biosynthesis of secondary metabolites
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SYSTEMATIC NAME
IUBMB Comments
D-glycerate:NADP+ 2-oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY hide
9059-44-3
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
physiological function
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deletion of isoform HPR3 results in slightly altered leaf concentrations of the photorespiratory intermediates HP, glycerate, and glycine, indicating a disrupted photorespiratory flux, but not in visible alteration of the phenotype.The combined deletion of of isoforms HPR1, HPR2, and HPR3 causes increased growth retardation, decreased photochemical efficiency, and reduced oxygen-dependent gas exchange in comparison with the hpr1hpr2 double mutant. Isoform HPR3 could provide a compensatory bypass for the reduction of hydroxypyruvate and glyoxylate within the chloroplast
evolution
the enzyme belongs to the beta-HAD (beta-hydroxyacid dehydrogenase) protein family. AtHPR2 and AtHPR3 are 45% identical to each other at the amino acid level, but only 19-25% identical to AtHPR1, the NADH-dependent form, and 8-9% identical to the AtGLYRs. None of the AtHPRs contains the active-site residues conserved in AtGLYR1 and AtGLYR2, indicating that the sites responsible for reducing glyoxylate differ greatly between the AtGLYRs and AtHPRs
physiological function
SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
glyoxylate + NADPH + H+
glycolate + NADP+
show the reaction diagram
-
-
-
?
hydroxypyruvate + NADH + H+
D-glycerate + NAD+
show the reaction diagram
isoform HPR3 prefers NADPH over NADH and glyoxylate over hydroxypyvruvate
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-
?
hydroxypyruvate + NADPH
D-glycerate + NADP+
show the reaction diagram
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-
-
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hydroxypyruvate + NADPH + H+
D-glycerate + NADP+
show the reaction diagram
isoform HPR3 prefers NADPH over NADH and glyoxylate over hydroxypyvruvate
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-
?
D-glycerate + NAD+
hydroxypyruvate + NADH + H+
show the reaction diagram
-
-
-
?
D-glycerate + NADP+
hydroxypyruvate + NADPH + H+
show the reaction diagram
-
-
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r
glyoxylate + NAD(P)H + H+
glycolate + NAD(P)+
show the reaction diagram
-
-
-
?
hydroxypyruvate + NAD(P)H + H+
D-glycerate + NAD(P)+
show the reaction diagram
-
-
-
?
hydroxypyruvate + NADH + H+
D-glycerate + NAD+
show the reaction diagram
hydroxypyruvate + NADPH
D-glycerate + NADP+
show the reaction diagram
-
-
-
?
hydroxypyruvate + NADPH + H+
D-glycerate + NADP+
show the reaction diagram
additional information
?
-
NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
glyoxylate + NADPH + H+
glycolate + NADP+
show the reaction diagram
Q9LE33
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-
-
?
hydroxypyruvate + NADPH
D-glycerate + NADP+
show the reaction diagram
Q9LE33
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D-glycerate + NAD+
hydroxypyruvate + NADH + H+
show the reaction diagram
A0A178WMD4, A0A1I9LPQ6, Q9CA90
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-
?
D-glycerate + NADP+
hydroxypyruvate + NADPH + H+
show the reaction diagram
A0A178WMD4, A0A1I9LPQ6, Q9CA90
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r
hydroxypyruvate + NAD(P)H + H+
D-glycerate + NAD(P)+
show the reaction diagram
Q9C9W5, Q9CA90
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?
additional information
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
isoform HPR3 prefers NADPH over NADH
NADPH
NADPH
additional information
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
oxalate
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
6.1
Hydroxypyruvate
GLYR1, at pH 7.8 and 22°C
kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.232
Hydroxypyruvate
GLYR1, at pH 7.8 and 22°C
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE TISSUE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
SOURCE
PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
43200
x * 43200, HPR2, SDS-PAGE
SUBUNITS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
?
x * 43200, HPR2, SDS-PAGE
Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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expressed in Escherichia coli
gene HPR3, sequence comparisons of GLYR genes and HPR genes
recombinant expression of GFP-tagged HPR2 in transgenic Arabidospsis thaliana plants
ENGINEERING
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
additional information