We're sorry, but BRENDA doesn't work properly without JavaScript. Please make sure you have JavaScript enabled in your browser settings.
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
EC Tree
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
L-lactaldehyde:propanediol oxidoreductase, Propanediol oxidoreductase, propanediol:nicotinamide adenine dinucleotide (NAD) oxidoreductase,
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
L-lactaldehyde:propanediol oxidoreductase
-
-
-
Propanediol oxidoreductase
-
-
-
propanediol:nicotinamide adenine dinucleotide (NAD) oxidoreductase
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(R)[or (S)]-propane-1,2-diol + NAD+ = (R)[or (S)]-lactaldehyde + NADH + H+
-
-
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(R)[or (S)]-propane-1,2-diol:NAD+ oxidoreductase
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(R)-propane-1,2-diol + NAD+
(R)-lactaldehyde + NADH
(S)-propane-1,2-diol + NAD+
(S)-lactaldehyde + NADH
DL-1,2-propanediol + NAD+
DL-lactaldehyde + NADH + H+
-
-
-
-
r
DL-lactaldehyde + NADH + H+
DL-1,2-propanediol + NAD+
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
ethylene glycol + NAD+
glycolaldehyde + NAD+
-
-
-
-
r
glycerol + NAD+
DL-glyceraldehyde + NADH
-
-
-
-
r
L-1,2-propanediol + NAD+
L-lactaldehyde + NADH + H+
propanol + NAD+
propionaldehyde + NADH
-
-
-
-
r
(R)-propane-1,2-diol + NAD+
(R)-lactaldehyde + NADH
-
-
-
-
?
(R)-propane-1,2-diol + NAD+
(R)-lactaldehyde + NADH
-
acts only on L-isomer
-
-
?
(R)-propane-1,2-diol + NAD+
(R)-lactaldehyde + NADH
-
-
-
-
?
(R)-propane-1,2-diol + NAD+
(R)-lactaldehyde + NADH
Microcyclus eburneus
-
-
-
-
?
(R)-propane-1,2-diol + NAD+
(R)-lactaldehyde + NADH
-
-
-
-
?
(R)-propane-1,2-diol + NAD+
(R)-lactaldehyde + NADH
-
utilizes D- and L-lactaldehyde in the reverse direction equally well
-
-
r
(S)-propane-1,2-diol + NAD+
(S)-lactaldehyde + NADH
-
-
-
-
r
(S)-propane-1,2-diol + NAD+
(S)-lactaldehyde + NADH
-
only L-propane-1,2-diol is oxidized
-
-
?
(S)-propane-1,2-diol + NAD+
(S)-lactaldehyde + NADH
-
anaerobically lactaldehyde is reduced to propane-1,2-diol in the L-fucose and L-rhamnose metabolism
-
-
?
(S)-propane-1,2-diol + NAD+
(S)-lactaldehyde + NADH
-
aerobically only mutant, strain 3 and 430, are capable of growth on 1,2-propanediol
-
-
?
(S)-propane-1,2-diol + NAD+
(S)-lactaldehyde + NADH
-
in the fermentative metabolism 1,2-propanediol is formed under phosphate limitation via the methylglyoxal by-pass
-
-
?
(S)-propane-1,2-diol + NAD+
(S)-lactaldehyde + NADH
Microcyclus eburneus
-
-
-
-
?
(S)-propane-1,2-diol + NAD+
(S)-lactaldehyde + NADH
-
-
-
-
?
(S)-propane-1,2-diol + NAD+
(S)-lactaldehyde + NADH
-
Embden-Meyerhof pathway for 6-deoxyhexose catabolism
-
-
?
(S)-propane-1,2-diol + NAD+
(S)-lactaldehyde + NADH
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
r
ethanol + NAD+
acetaldehyde + NADH
-
-
-
-
r
L-1,2-propanediol + NAD+
L-lactaldehyde + NADH + H+
-
-
-
-
?
L-1,2-propanediol + NAD+
L-lactaldehyde + NADH + H+
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
(S)-propane-1,2-diol + NAD+
(S)-lactaldehyde + NADH
L-1,2-propanediol + NAD+
L-lactaldehyde + NADH + H+
(S)-propane-1,2-diol + NAD+
(S)-lactaldehyde + NADH
-
-
-
-
r
(S)-propane-1,2-diol + NAD+
(S)-lactaldehyde + NADH
-
anaerobically lactaldehyde is reduced to propane-1,2-diol in the L-fucose and L-rhamnose metabolism
-
-
?
(S)-propane-1,2-diol + NAD+
(S)-lactaldehyde + NADH
-
aerobically only mutant, strain 3 and 430, are capable of growth on 1,2-propanediol
-
-
?
(S)-propane-1,2-diol + NAD+
(S)-lactaldehyde + NADH
-
in the fermentative metabolism 1,2-propanediol is formed under phosphate limitation via the methylglyoxal by-pass
-
-
?
(S)-propane-1,2-diol + NAD+
(S)-lactaldehyde + NADH
-
Embden-Meyerhof pathway for 6-deoxyhexose catabolism
-
-
?
L-1,2-propanediol + NAD+
L-lactaldehyde + NADH + H+
-
-
-
-
?
L-1,2-propanediol + NAD+
L-lactaldehyde + NADH + H+
-
-
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NAD+
-
-
NAD+
Microcyclus eburneus
-
-
NADH
-
-
NADP+
-
cell extracts also contain lactaldehyde reductase activity which utilizes either NADH or NADPH as electron donor
NADP+
-
one tenth of the activity as with NAD+
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
ascorbate
-
0.025 mM FeCl3 and 0.035 mM ascorbate causes a 90% enzyme inactivation after 120 min = inactivation of metal-catalyzed oxidation; several amino-acids prevent the inactivation; under aerobic conditions 0.025 mM FeCl3 causes a 50% enzyme inactivation after 120 min
Fe2+
-
under anaerobic conditions 0.01 mM Fe2+ and 0.1 mM H2O2 had the effect of 85% enzyme inactivation after 2 min, with addition of 0.0001 mM catalase the inactivation is only 25% after 2 min and with addition of 0.01 mM superoxide dismutase instead of catalse the inactivation is again 82% after 2 min
Fe3+
-
0.025 mM FeCl3 and 0.035 mM ascorbate causes a 90% enzyme inactivation after 120 min = inactivation of metal-catalyzed oxidation; several amino-acids prevent the inactivation; under aerobic conditions 0.025 mM FeCl3 causes a 50% enzyme inactivation after 120 min
H2O2
-
under anaerobic conditions 0.01 mM Fe2+ and 0.1 mM H2O2 had the effect of 85% enzyme inactivation after 2 min, with addition of 0.0001 mM catalase the inactivation is only 25% after 2 min and with addition of 0.01 mM superoxide dismutase instead of catalse the inactivation is again 82% after 2 min
L-1,2-propanediol
-
above 25 mM: substrate inhibition
p-chloromercuribenzoate
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Infections
Lack of influence of the anaerobic [NiFe] hydrogenase and L-1,2 propanediol oxidoreductase on the outcome of Actinobacillus pleuropneumoniae serotype 7 infection.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
11
DL-1,2-Propanediol
-
-
1.25
L-1,2-propanediol
-
pH 9.5
0.035
L-lactaldehyde
-
pH 7.0
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
36.6
Microcyclus eburneus
-
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
9.5
-
dehydrogenation of L-1,2-propanediol
6.5
-
reduction of L-lactaldehyde
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
4.5 - 10
-
reduction of lactaldehyde, pH 4.5: 50% of reductase activity maximum, pH 10: 10% of reductase activity maximum
6 - 7.8
-
reduction of DL-lactaldehyde, pH 6: 50% of reductase activity maximum, pH 7.8: 45% of reductase activity maximum
8 - 11
-
dehydrogenation of 1,2-propanediol, pH 8: 10% of dehydrogenase activity maximum, pH 11: 65% of dehydrogenase activity maximum
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
25
-
enzyme assay performed at this temperature
30
-
enzyme assay performed at this temperature
43
-
enzyme assay performed at this temperature
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
25 - 43
-
optimal temperature for enzyme activity differs in various organisms
25 - 43
-
optimal temperature for enzyme activity differs in various organisms
25 - 43
-
optimal temperature for enzyme activity differs in various organisms
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
gene rhaO
-
-
brenda
gene rhaO
-
-
brenda
-
-
-
brenda
-
-
-
brenda
Microcyclus eburneus
-
-
-
brenda
-
-
-
brenda
-
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
-
-
brenda
-
-
brenda
-
-
brenda
-
-
brenda
-
-
brenda
-
-
brenda
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
155000
Microcyclus eburneus
-
gel filtration on Sephadex G-200
38000
Microcyclus eburneus
-
4 * 38000, SDS-PAGE
39000
-
2 * 39000, SDS-PAGE
76000
-
gel permeation chromatography on a calibrated column of Ultrogel AcA 34 under denaturating conditions
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
dimer
-
2 * 39000, SDS-PAGE
tetramer
Microcyclus eburneus
-
4 * 38000, SDS-PAGE
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
additional information
-
a rhaO knockout mutant produces reduced levels of L-1,2-propanediol during growth in rhamnose, the mutant cannot utilise rhamnose as the sole carbon source but grows normally on glucose
additional information
-
a rhaO knockout mutant produces reduced levels of L-1,2-propanediol during growth in rhamnose, the mutant cannot utilise rhamnose as the sole carbon source but grows normally on glucose
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
crude extract, ammonium sulfate precipitation, heat treatment, ultrogel AcA 34 fractionation, DEAE-Sephadex column chromatography, second ultrogel AcA 34 fractionation
-
crude extract, protamine sulfate, ammonium sulfate, DEAE-Sephadex-25, hydroxylapatite, blue dextran polyacrylamide
Microcyclus eburneus
-
extraction, heat treatment, ammonium sulfate-fractionation, DEAE-cellulose and Alumina C
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
gene rhaO, encoded in the rhaKIPAO gene cluster, expression analysis, transcriptional regulation of the rhaKIPAOR gene cluster, overview
-
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
synthesis
-
fermentation of L-rhamnose, L-fucose and D-fucose to a mixture of 1,2-propanediol, acetone, H2, CO2 and ethanol
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
Tran-Din, K.; Gottschalk, G.
Formation of D(-)-1,2-propanediol and D(-)-lactate from glucose by Clostridium sphenoides under phosphate limitation
Arch. Microbiol.
142
87-92
1985
Lacrimispora sphenoides
-
brenda
Ting, S.M.; Sellinger, O.Z.; Miller, O.N.
The metabolism of lactaldehyde VI. The reduction of D- and L-lactaldehyde in rat liver
Biochim. Biophys. Acta
89
217-225
1965
Rattus norvegicus
brenda
Kawagishi, T.; Nishio, N.; Matsuno, R.; Kamikubo, T.
Purification of NAD-dependent 1,2-propanediol dehydrogenating enzyme from Microcyclus eberneus
Agric. Biol. Chem.
44
949-950
1980
Microcyclus eburneus
-
brenda
Weimer, P.J.
Fermentation of 6-deoxyhexoses by Bacillus macerans
Appl. Environ. Microbiol.
47
263-267
1984
Paenibacillus macerans
brenda
Boronat, A.; Aguilar,J.
Rhamnose-induced propanediol oxidoreductase in Escherichia coli: purification, properties and comparison with the fucose-induced enzyme
J. Bacteriol.
140
320-326
1979
Escherichia coli
brenda
Hacking, A.J.; Lin, E.C.C.
Disruption of the fucose pathway as a consequence of genetic adaptation to propanediol as a carbon source in Escherichia coli
J. Bacteriol.
126
1166-1172
1976
Escherichia coli
brenda
Hacking, A.J.; Aguilar, J.; Lin, E.C.C.
Evolution of propanediol utilization in Escherichia coli: mutant with improved substrate-scavenging power
J. Bacteriol.
136
522-530
1978
Escherichia coli
brenda
Cocks, G.T.; Aguilar, J.; Lin, E.C.C.
Evolution of L-1,2-propanediol catabolism in Escherichia coli by recruitment of enzymes for L-fucose and L-lactate metabolism
J. Bacteriol.
118
83-88
1974
Escherichia coli
brenda
Boronat, A.; Aguilar, J.
Metabolism of L-fucose and L-rhamnose in Escherichia coli; differences in induction of propanediol oxidoreductase
J. Bacteriol.
147
181-185
1981
Escherichia coli
brenda
Cabiscol, E.; Badia, J.; Baldoma, L.; Hidalgo, E.; Aguilar, J.; Ros, J.
Inactivation of propanediol oxidoreductase of Escherichia coli by metal-catalyzed oxidation
Biochim. Biophys. Acta
1118
155-160
1992
Escherichia coli
brenda
Patel, E.H.; Paul, L.V.; Patrick, S.; Abratt, V.R.
Rhamnose catabolism in Bacteroides thetaiotaomicron is controlled by the positive transcriptional regulator RhaR
Res. Microbiol.
159
678-684
2008
Bacteroides thetaiotaomicron, Bacteroides thetaiotaomicron VPI-5482
brenda
Select items on the left to see more content.
html completed
HOME
login
history
all enzymes
BRENDA home
History
1.1.1.77 lactaldehyde reductase
more
top
Information
