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Information on EC 1.1.1.65 - pyridoxine 4-dehydrogenase and Organism(s) Schizosaccharomyces pombe and UniProt Accession O14295

for references in articles please use BRENDA:EC1.1.1.65
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IUBMB Comments
Also oxidizes pyridoxine phosphate.
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This record set is specific for:
Schizosaccharomyces pombe
UNIPROT: O14295
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The taxonomic range for the selected organisms is: Schizosaccharomyces pombe
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Reaction Schemes
Synonyms
pl reductase, pyridoxine dehydrogenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
dehydrogenase, pyridoxol 4-
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PL reductase
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PL-red
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pyridoxin dehydrogenase
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pyridoxine dehydrogenase
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pyridoxol dehydrogenase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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oxidation
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reduction
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SYSTEMATIC NAME
IUBMB Comments
pyridoxine:NADP+ 4-oxidoreductase
Also oxidizes pyridoxine phosphate.
CAS REGISTRY NUMBER
COMMENTARY hide
9028-64-2
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-nitrobenzaldehyde + NADPH
2-nitrobenzyl alcohol + NADP+
show the reaction diagram
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-
?
pyridoxine + NADP+
pyridoxal + NADPH
show the reaction diagram
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r
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
pyridoxine + NADP+
pyridoxal + NADPH
show the reaction diagram
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r
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.096
2-nitrobenzaldehyde
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7.5
for formation of pyridoxine
7.5 - 8.5
for formation of pyridoxal
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41000
gel filtration
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
purified enzyme very unstable but can be stabilized by low concentrations of various detergents such as Tween 40
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Nakano, M.; Morita, T.; Yamamoto, T.; Sano, H.; Ashiuchi, M.; Masui, R.; Kuramitsu, S.; Toshiharu, Y.
Purification, molecular cloning, and catalytic activity of Schizosaccharomyces pombe pyridoxal reductase
J. Biol. Chem.
274
23185-23190
1999
Schizosaccharomyces pombe (O14295), Schizosaccharomyces pombe
Manually annotated by BRENDA team