Information on EC 1.1.1.65 - pyridoxine 4-dehydrogenase

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The enzyme appears in viruses and cellular organisms

top print hide
EC NUMBER
COMMENTARY hide
1.1.1.65
-
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RECOMMENDED NAME
GeneOntology No.
pyridoxine 4-dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
pyridoxine + NADP+ = pyridoxal + NADPH + H+
show the reaction diagram
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-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
oxidation
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-
-
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redox reaction
-
-
-
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reduction
-
-
-
-
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
4-amino-2-methyl-5-diphosphomethylpyrimidine biosynthesis (yeast)
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pyridoxal 5'-phosphate salvage II (plants)
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Vitamin B6 metabolism
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Metabolic pathways
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SYSTEMATIC NAME
IUBMB Comments
pyridoxine:NADP+ 4-oxidoreductase
Also oxidizes pyridoxine phosphate.
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CAS REGISTRY NUMBER
COMMENTARY hide
9028-64-2
-
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-nitrobenzaldehyde + NADPH
2-nitrobenzyl alcohol + NADP+
show the reaction diagram
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-
-
?
pyridoxine + NADP+
pyridoxal + NADPH
show the reaction diagram
pyridoxine phosphate + NADP+
pyridoxal 5'-phosphate + NADPH
show the reaction diagram
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-
-
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
pyridoxine + NADP+
pyridoxal + NADPH
show the reaction diagram
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ZnCl2
-
0.1 mM: activation, 10 mM: inhibition
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INHIBITORS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
p-Chloromercuriphenyl sulfonic acid
-
-
ZnCl2
-
0.1 mM: activation, 10 mM: inhibition
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.096
2-nitrobenzaldehyde
-
0.9 - 1.6
pyridoxal
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
6.5 - 7.5
for formation of pyridoxine
7.5 - 8.5
for formation of pyridoxal
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
5 - 8
-
at pH 5.0 and 8.0 about 50% of activity maximum
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
41000
gel filtration
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
purified enzyme very unstable but can be stabilized by low concentrations of various detergents such as Tween 40
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Purification/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
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Show AA Sequence (199 entries)
Please use the Sequence Search for a specific query.
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LINKS TO OTHER DATABASES (specific for EC-Number 1.1.1.65)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)