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Information on EC 1.1.1.53 - 3alpha(or 20beta)-hydroxysteroid dehydrogenase and Organism(s) Mycobacterium tuberculosis and UniProt Accession P9WGT1

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EC Tree
IUBMB Comments
The 3alpha-hydroxy group or 20beta-hydroxy group of pregnane and androstane steroids can act as donor.
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Select one or more organisms in this record: ?
This record set is specific for:
Mycobacterium tuberculosis
UNIPROT: P9WGT1
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Word Map
The taxonomic range for the selected organisms is: Mycobacterium tuberculosis
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
20beta-hsd, cortisone reductase, 3alpha-hsd/cr, 3alpha-hydroxysteroid dehydrogenase/carbonyl reductase, 20-hydroxysteroid dehydrogenase, rodh2, 20hsd, cr/20beta-hsd, 20beta-hsd type 2, cr/20beta-hsd a, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
(R)-20-hydroxysteroid dehydrogenase
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20beta-HSD
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20beta-hydroxysteroid dehydrogenase
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3alpha,20beta-hydroxysteroid:NAD+-oxidoreductase
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cortisone reductase
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dehydrogenase, 20beta-hydroxy steroid
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delta4-3-ketosteroid hydrogenase
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NADH-20beta-hydroxysteroid dehydrogenase
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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oxidation
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reduction
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PATHWAY SOURCE
PATHWAYS
SYSTEMATIC NAME
IUBMB Comments
3alpha(or 20beta)-hydroxysteroid:NAD+ oxidoreductase
The 3alpha-hydroxy group or 20beta-hydroxy group of pregnane and androstane steroids can act as donor.
CAS REGISTRY NUMBER
COMMENTARY hide
9028-42-6
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
17beta-estradiol + NAD+
estrone + NADH + H+
show the reaction diagram
4% of the activity with androsterone
-
-
?
20alpha-hydroxyprogesterone + NAD+
? + NADH
show the reaction diagram
2% of the activity with androsterone
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-
?
20beta-hydroxyprogesterone + NAD+
? + NADH
show the reaction diagram
69% of the activity with androsterone
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-
?
androsterone + NAD+
? + NADH
show the reaction diagram
-
-
-
?
epiandrosterone + NAD+
? + NADH
show the reaction diagram
3% of the activity with androsterone
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-
?
progesterone + NADH
20beta-hydroxy-pregn-4-ene-3-one + NAD+
show the reaction diagram
22% of the activity with androsterone
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-
?
COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
NADH
preference of NADH over NADPH
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.017
20beta-hydroxyprogesterone
pH 6.0, 30°C, reaction with NAD+, mutant enzyme I6T/V47M/T69K
0.024
androsterone
pH 6.0, 30°C, reaction with NAD+, mutant enzyme I6T/V47M/T69K
0.0033
progesterone
pH 6.0, 30°C, reaction with NADH, mutant enzyme I6T/V47M/T69K
TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
258
20beta-hydroxyprogesterone
pH 6.0, 30°C, reaction with NAD+
456
androsterone
pH 6.0, 30°C, reaction with NAD+
72
progesterone
pH 6.0, 30°C, reaction with NADH
pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10
oxidation of androsterone shows a dual pH-optimum, one at pH 6.0-6.5 and the other at pH 10, mutant enzyme I6T/V47M/T69K/E142A
6
progesterone + NADH, mutant enzyme I6T/V47M/T69K
6 - 6.5
oxidation of androsterone shows a dual pH-optimum, one at pH 6.0-6.5 and the other at pH 10, mutant enzyme I6T/V47M/T69K/E142A
6.5
progesterone + NADH, mutant enzyme I6T/V47M/T69K/E142A
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
Rv2002
Uniprot
Manually annotated by BRENDA team
CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structure of the mutant enzyme I6T/V47M/T69K in binary complex with NAD+ at 1.8 A resolution and as a ternary complex with androsterone and NADH at 2.4 A
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
I6T/V47M/T69K
mutant enzyme with a maximal improvement in solubility, high catalytic activity as a NADP-dependent 3alpha,20beta-hydroxysteroid dehydrogenase
I6T/V47M/T69K/E142A
pH optimum for reduction of progesterone shifts slightly from pH 6.0 to pH 6.5 compared to for mutant enzyme I6T/V47M/T69K, the oxidation of androsterone shows a dual pH optimum, one at pH 6.0-6.5 and the other at pH 10
I6T/V47M/T69K/S140A
both oxidation of androsterone and reduction of progesterone are completely lost
I6T/V47M/T69K/Y153F
both oxidation of androsterone and reduction of progesterone are completely lost
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
selenomethionine-substituted mutant enzyme I6T/V47M/T69K, expression in inclusion bodies in Escherichia coli B834(DE3)
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Yang, J.K.; Park, M.S.; Waldo, G.S.; Suh, S.W.
Directed evolution approach to a structural genomics project: Rv2002 from Mycobacterium tuberculosis
Proc. Natl. Acad. Sci. USA
100
455-460
2003
Mycobacterium tuberculosis (P9WGT1), Mycobacterium tuberculosis H37Rv (P9WGT1)
Manually annotated by BRENDA team