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Information on EC 1.1.1.47 - glucose 1-dehydrogenase [NAD(P)+] and Organism(s) Haloferax mediterranei and UniProt Accession Q977U7

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EC Tree
IUBMB Comments
This enzyme has similar activity with either NAD+ or NADP+. cf. EC 1.1.1.118, glucose 1-dehydrogenase (NAD+) and EC 1.1.1.119, glucose 1-dehydrogenase (NADP+).
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This record set is specific for:
Haloferax mediterranei
UNIPROT: Q977U7
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Word Map
The taxonomic range for the selected organisms is: Haloferax mediterranei
The expected taxonomic range for this enzyme is: Bacteria, Archaea, Eukaryota
Synonyms
hexose-6-phosphate dehydrogenase, glcdh-i, glcdh-ii, ssgdh, glcdh-iwg3, bmglcdh-iv, lsgdh, bzgdh, hexose phosphate dehydrogenase, nad(p)-dependent glucose dehydrogenase, more
SYNONYM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
beta-D-glucose:NAD(P)+ 1-oxidoreductase
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D-glucose dehydrogenase (NAD(P))
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general stress protein 74
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GlcDH
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glucose dehydrogenase
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GSP74
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hexose phosphate dehydrogenase
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hexose-6-phosphate dehydrogenase
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
D-glucose + NAD(P)+ = D-glucono-1,5-lactone + NAD(P)H + H+
show the reaction diagram
sequential type ordered bibi mechnanism
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
redox reaction
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oxidation
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reduction
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SYSTEMATIC NAME
IUBMB Comments
D-glucose:NAD(P)+ 1-oxidoreductase
This enzyme has similar activity with either NAD+ or NADP+. cf. EC 1.1.1.118, glucose 1-dehydrogenase (NAD+) and EC 1.1.1.119, glucose 1-dehydrogenase (NADP+).
CAS REGISTRY NUMBER
COMMENTARY hide
9028-53-9
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
beta-D-glucose + NAD+
D-glucono-1,5-lactone + NADH + H+
show the reaction diagram
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
METALS and IONS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
MgCl2
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25 mM for activity assay
NaCl
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2 M for activity assay
additional information
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extreme halophilic enzyme
INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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hexadecyltrimethylammonium bromide, i.e. CTAB, does not influence the enzyme activity but increases the enzyme stability
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
additional information
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hexadecyltrimethylammonium bromide, i.e. CTAB, does not influence the enzyme activity but increases the enzyme stability
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
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SwissProt
Manually annotated by BRENDA team
LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY hide
GeneOntology No.
LITERATURE
SOURCE
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
enzyme shows good stability at low water content and at low salt concentration in reverse micelles
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ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
cyclohexane
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enzyme is solubilized in reverse micelles of hexadecyltrimethylammonium bromide in cyclohexane with n-butanol as cosurfactant and retaines activity and catalytic properties in the organic medium
n-Butanol
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enzyme is solubilized in reverse micelles of hexadecyltrimethylammonium bromide in cyclohexane with n-butanol as cosurfactant and retaines activity and catalytic properties in the organic medium
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant enzyme is solubilized in reverse micelles of hexadecyltrimethylammonium bromide, i.e. CTAB, in cyclohexane with n-butanol as cosurfactant
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
synthesis
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enzyme can be used for gluconic acid production in low water systems
REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Pire, C.; Marhuenda-Egea, F.C.; Esclapez, J.; Alcaraz, L.; Ferrer, J.; Bonete, M.J.
Stability and enzymatic studies of glucose dehydrogenase from the archaeon Haloferax mediterranei in reverse micelles
Biocatal. Biotransform.
22
17-23
2004
Haloferax mediterranei
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Manually annotated by BRENDA team
Britton, K.L.; Baker, P.J.; Fisher, M.; Ruzheinikov, S.; Gilmour, D.J.; Bonete, M.J.; Ferrer, J.; Pire, C.; Esclapez, J.; Rice, D.W.
Analysis of protein solvent interactions in glucose dehydrogenase from the extreme halophile Haloferax mediterranei
Proc. Natl. Acad. Sci. USA
103
4846-4851
2006
Haloferax mediterranei (Q977U7), Haloferax mediterranei DSM 1411 (Q977U7)
Manually annotated by BRENDA team