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EC Tree
IUBMB Comments The enzyme, characterized from several bacterial species, participates in the degradation of D-apionate. The reaction involves migration of a hydroxymethyl group from position 3 to position 2 and oxidation of the 3-hydroxyl group. Stereospecificity of the product, 3-oxoisoapionate, has not been determined.
The enzyme appears in viruses and cellular organisms
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D-apionate + NAD+ = 3-oxo-D-isoapionate + NADH + H+
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-
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-
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D-apionate:NAD+ oxidoreductase (isomerizing)
The enzyme, characterized from several bacterial species, participates in the degradation of D-apionate. The reaction involves migration of a hydroxymethyl group from position 3 to position 2 and oxidation of the 3-hydroxyl group. Stereospecificity of the product, 3-oxoisoapionate, has not been determined.
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D-apionate + NAD+
3-oxo-D-isoapionate + NADH + H+
D-apionate + NAD+
3-oxo-D-isoapionate + NADH + H+
-
-
-
r
D-apionate + NAD+
3-oxo-D-isoapionate + NADH + H+
-
-
-
r
D-apionate + NAD+
3-oxo-D-isoapionate + NADH + H+
-
-
-
r
D-apionate + NAD+
3-oxo-D-isoapionate + NADH + H+
-
-
-
r
D-apionate + NAD+
3-oxo-D-isoapionate + NADH + H+
-
-
-
r
D-apionate + NAD+
3-oxo-D-isoapionate + NADH + H+
-
-
-
r
D-apionate + NAD+
3-oxo-D-isoapionate + NADH + H+
-
-
-
r
D-apionate + NAD+
3-oxo-D-isoapionate + NADH + H+
-
-
-
r
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D-apionate + NAD+
3-oxo-D-isoapionate + NADH + H+
D-apionate + NAD+
3-oxo-D-isoapionate + NADH + H+
-
-
-
r
D-apionate + NAD+
3-oxo-D-isoapionate + NADH + H+
-
-
-
r
D-apionate + NAD+
3-oxo-D-isoapionate + NADH + H+
-
-
-
r
D-apionate + NAD+
3-oxo-D-isoapionate + NADH + H+
-
-
-
r
D-apionate + NAD+
3-oxo-D-isoapionate + NADH + H+
-
-
-
r
D-apionate + NAD+
3-oxo-D-isoapionate + NADH + H+
-
-
-
r
D-apionate + NAD+
3-oxo-D-isoapionate + NADH + H+
-
-
-
r
D-apionate + NAD+
3-oxo-D-isoapionate + NADH + H+
-
-
-
r
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NAD+
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NADH
-
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UniProt
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APNO_AGRRK
Agrobacterium radiobacter (strain K84 / ATCC BAA-868)
275
0
29724
Swiss-Prot
-
APNO_BLAHS
Blautia hydrogenotrophica (strain DSM 10507 / JCM 14656 / S5a33)
287
0
31231
Swiss-Prot
-
APNO_CUPNN
Cupriavidus necator (strain ATCC 43291 / DSM 13513 / CCUG 52238 / LMG 8453 / N-1)
276
0
30279
Swiss-Prot
-
APNO_PECAS
Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)
285
0
30932
Swiss-Prot
-
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Ni-NTA resin column chromatography
Ni-NTA resin column chromatography
Ni-NTA resin column chromatography
Ni-NTA resin column chromatography
Ni-NTA resin column chromatography
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expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) cells
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Carter, M.; Zhang, X.; Huang, H.; Bouvier, J.; Francisco, B.; Vetting, M.; Al-Obaidi, N.; Bonanno, J.; Ghosh, A.; Zallot, R.; Andersen, H.; Almo, S.; Gerlt, J.
Functional assignment of multiple catabolic pathways for D-apiose article
Nat. Chem. Biol.
14
696-705
2018
Agrobacterium tumefaciens (B9JK75), Blautia hydrogenotrophica (C0CMQ7), Cupriavidus necator (F8GV06), Pectobacterium atrosepticum (Q6D8V3), Agrobacterium tumefaciens K84 (B9JK75), Cupriavidus necator ATCC 43291 (F8GV06), Blautia hydrogenotrophica DSM 10507 (C0CMQ7), Pectobacterium atrosepticum SCRI 1043 (Q6D8V3)
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