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IUBMB Comments The enzyme, characterized from the plant Papaver somniferum (opium poppy), catalyses the last step in the biosynthesis of the isoquinoline alkaloid noscapine.
Synonyms
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short-chain dehydrogenase/reductase
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NOS
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narcotine hemiacetal + NAD+ = noscapine + NADH + H+
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MetaCyc
noscapine biosynthesis
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narcotine hemiacetal:NAD+ 1-oxidoreductase
The enzyme, characterized from the plant Papaver somniferum (opium poppy), catalyses the last step in the biosynthesis of the isoquinoline alkaloid noscapine.
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narcotine hemiacetal + NAD+
noscapine + NADH + H+
narcotine hemiacetal + NADP+
noscapine + NADPH + H+
Substrates: the enzyme exhibits higher catalytic efficiency with NAD+ as the cofactor compared with NADP+
r
noscapine + NADH + H+
narcotine hemiacetal + NAD+
Substrates: -
r
noscapine + NADPH + H+
narcotine hemiacetal + NADP+
Substrates: -
r
narcotine hemiacetal + NAD+
noscapine + NADH + H+
Substrates: -
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narcotine hemiacetal + NAD+
noscapine + NADH + H+
Substrates: the enzyme catalyses the last step in the biosynthesis of the isoquinoline alkaloid noscapine
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narcotine hemiacetal + NAD+
noscapine + NADH + H+
Substrates: the enzyme exhibits higher catalytic efficiency with NAD+ as the cofactor compared with NADP+
r
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narcotine hemiacetal + NAD+
noscapine + NADH + H+
narcotine hemiacetal + NAD+
noscapine + NADH + H+
Substrates: -
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narcotine hemiacetal + NAD+
noscapine + NADH + H+
Substrates: the enzyme catalyses the last step in the biosynthesis of the isoquinoline alkaloid noscapine
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NAD+
the enzyme exhibits higher catalytic efficiency with NAD+ as the cofactor compared with NADP+
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0.0209 - 0.268
narcotine hemiacetal
0.0209
narcotine hemiacetal
cofactor NAD+, pH 8.0, 30°C
0.268
narcotine hemiacetal
cofactor NADP+, pH 8.0, 30°C
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0.59 - 1.69
narcotine hemiacetal
0.59
narcotine hemiacetal
pH 8.0, 30°C, cofactor NAD+
1.69
narcotine hemiacetal
pH 8.0, 30°C, cofactor NADP+
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6.296 - 28.097
narcotine hemiacetal
6.296
narcotine hemiacetal
cofactor NADP+, pH 8.0, 30°C
28.097
narcotine hemiacetal
cofactor NAD+, pH 8.0, 30°C
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UniProt
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intermediate level
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intermediate level
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least abundant in roots
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NOS transcripts are detected in all opium poppy organs, but are most abundant in stems
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Highest Expressing Human Cell Lines
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Cell Line Links
Gene Links
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malfunction
suppression of NOS transcript levels in opium poppy plants subjected to virus-induced gene silencing results in a corresponding reduction in the accumulation of noscapine and an increase in narcotinehemiacetal levels in the latex
metabolism
the enzyme catalyses the last step in the biosynthesis of the isoquinoline alkaloid noscapine
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SDR1_PAPSO
348
1
38815
Swiss-Prot
other Location (Reliability: 2 )
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expressed in Escherichia coli
Saccharomyces cerevisiae CSY1060 and CSY1061 strains are used as the base strain for construction of the de novo noscapine biosynthetic strains
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synthesis
complete biosynthesis of noscapine and halogenated alkaloids in yeast and optimizing noscapine production toward scalable manufacturing. Engineered strain contains 25 heterologous plant, bacteria, and mammalian genes and 6 mutant or overexpressed yeast genes. The noscapine biosynthetic pathway incorporates seven endomembrane-localized plant enzymes, highlighting the ability of the yeast to functionally express and properly localize large numbers of heterologous enzymes into the endoplasm reticulum. Noscapine titers were improved by 18000fold (to low mg/l levels) via a combination of enzyme engineering, pathway and strain engineering, and fermentation optimization. Microbial fermentation can be used to produce halogenated alkaloid derivatives, which can ultimately serve as potential drug leads, through feeding amino acid derivatives to strains
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Chen, X.; Facchini, P.J.
Short-chain dehydrogenase/reductase catalyzing the final step of noscapine biosynthesis is localized to laticifers in opium poppy
Plant J.
77
173-184
2014
Papaver somniferum (I3PLR3), Papaver somniferum
brenda
Li, Y.; Li, S.; Thodey, K.; Trenchard, I.; Cravens, A.; Smolke, C.
Complete biosynthesis of noscapine and halogenated alkaloids in yeast
Proc. Natl. Acad. Sci. USA
115
E3922-E3931
2018
Papaver somniferum (I3PLR3)
brenda
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