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EC Tree
IUBMB Comments This bacterial enzyme is involved, along with EC 1.1.1.52, 3alpha-hydroxycholanate dehydrogenase (NAD+), or EC 1.1.1.392, 3alpha-hydroxycholanate dehydrogenase (NADP+), in the modification of secondary bile acids to form 3beta-bile acids (also known as iso-bile acids). The enzyme catalyses the reaction in the reduction direction in vivo. Also acts on related 3-oxo bile acids. cf. EC 1.1.1.391, 3beta-hydroxycholanate 3-dehydrogenase (NAD+).
The enzyme appears in viruses and cellular organisms
Synonyms
3beta-HSDH, 3beta-hydroxysteroid dehydrogenase, 3beta-hyoxysteroid dehydrogenase,
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3beta-hydroxysteroid dehydrogenase
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ambiguous
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3beta-hyoxysteroid dehydrogenase
3beta-HSDH
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3beta-hyoxysteroid dehydrogenase
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3beta-hyoxysteroid dehydrogenase
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isolithocholate + NADP+ = 3-oxo-5beta-cholan-24-oate + NADPH + H+
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isolithocholate:NADP+ 3-oxidoreductase
This bacterial enzyme is involved, along with EC 1.1.1.52, 3alpha-hydroxycholanate dehydrogenase (NAD+), or EC 1.1.1.392, 3alpha-hydroxycholanate dehydrogenase (NADP+), in the modification of secondary bile acids to form 3beta-bile acids (also known as iso-bile acids). The enzyme catalyses the reaction in the reduction direction in vivo. Also acts on related 3-oxo bile acids. cf. EC 1.1.1.391, 3beta-hydroxycholanate 3-dehydrogenase (NAD+).
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12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+
3,7,12-trioxo-5beta-cholan-24-oic acid + NADPH + H+
3beta-hydroxy-7,12-dioxo-5beta-cholan-24-oic acid + NADP+
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+
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12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+
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3,7,12-trioxo-5beta-cholan-24-oic acid + NADPH + H+
3beta-hydroxy-7,12-dioxo-5beta-cholan-24-oic acid + NADP+
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3,7,12-trioxo-5beta-cholan-24-oic acid + NADPH + H+
3beta-hydroxy-7,12-dioxo-5beta-cholan-24-oic acid + NADP+
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3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+
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3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+
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12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+
3,7,12-trioxo-5beta-cholan-24-oic acid + NADPH + H+
3beta-hydroxy-7,12-dioxo-5beta-cholan-24-oic acid + NADP+
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+
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12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+
3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+
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3,7,12-trioxo-5beta-cholan-24-oic acid + NADPH + H+
3beta-hydroxy-7,12-dioxo-5beta-cholan-24-oic acid + NADP+
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3,7,12-trioxo-5beta-cholan-24-oic acid + NADPH + H+
3beta-hydroxy-7,12-dioxo-5beta-cholan-24-oic acid + NADP+
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3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+
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3beta,12alpha-dihydroxy-5beta-cholan-24-oic acid + NADP+
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADPH + H+
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NADPH
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0.056
12alpha-Hydroxy-3-oxo-5beta-cholan-24-oic acid
pH 7.0, 37ưC
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157.2
12alpha-Hydroxy-3-oxo-5beta-cholan-24-oic acid
pH 7.0, 37ưC
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37
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assay at
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isolated from human intestine
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brenda
isolated from human intestine
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gene RUMGNA_00694
UniProt
brenda
gene RUMGNA_00694
UniProt
brenda
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brenda
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brenda
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brenda
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metabolism
the enzyme is involved in the biosynthetic pathway for the second most abundant class in the gut, 3beta-hydroxy(iso)bile acids
metabolism
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the organism also possesses 3beta-hyoxysteroid dehydrogenase activity that is active with the same substrate as the 3beta-hyoxysteroid dehydrogenase but is specific for 7beta-hydroxy groups, overview
metabolism
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the organism also possesses 3beta-hyoxysteroid dehydrogenase activity that is active with the same substrate as the 3beta-hyoxysteroid dehydrogenase but is specific for 7beta-hydroxy groups, overview
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metabolism
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the enzyme is involved in the biosynthetic pathway for the second most abundant class in the gut, 3beta-hydroxy(iso)bile acids
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physiological function
the iso-bile acid pathway detoxifies deoxycholic acid and thus favors the growth of the keystone genus Bacteroides
physiological function
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the iso-bile acid pathway detoxifies deoxycholic acid and thus favors the growth of the keystone genus Bacteroides
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3BHDP_RUMGV
Ruminococcus gnavus (strain ATCC 29149 / VPI C7-9)
276
0
29047
Swiss-Prot
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A0A5S9QBJ1_9GAMM
300
0
31846
TrEMBL
-
A0A6J4ZVH4_9BURK
254
0
26745
TrEMBL
-
A0A5S9PQZ6_9GAMM
286
0
30152
TrEMBL
-
A0A6J5DHT2_9BURK
257
0
26433
TrEMBL
-
A0A5S9NRK6_9GAMM
318
0
34278
TrEMBL
-
A0A7K0DYK5_9NOCA
254
0
25661
TrEMBL
-
A0A5E7CKK9_PSEFL
261
0
27827
TrEMBL
-
A0A2N7WT22_9BURK
254
0
26823
TrEMBL
-
A0A5E6TJ47_PSEFL
254
0
26719
TrEMBL
-
A0A6G8NZQ9_9BURK
258
0
26903
TrEMBL
-
A0A5E7NM73_PSEFL
254
0
26642
TrEMBL
-
A0A5E7VD92_PSEFL
254
0
26692
TrEMBL
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A0A645IW62_9ZZZZ
76
0
8154
TrEMBL
other Location (Reliability: 1 )
A0A6M8MTA4_9PSED
254
0
26626
TrEMBL
-
A0A6J5KD74_9BURK
254
0
26654
TrEMBL
-
B1FZZ0_PARG4
Paraburkholderia graminis (strain ATCC 700544 / DSM 17151 / LMG 18924 / NCIMB 13744 / C4D1M)
254
0
26792
TrEMBL
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A0A6S7BF64_9BURK
258
0
27021
TrEMBL
-
A0A5E6PVP5_PSEFL
253
0
27124
TrEMBL
-
A0A1X1PNA9_9BURK
252
0
26472
TrEMBL
-
A0A5S9N3B2_9GAMM
300
0
31878
TrEMBL
-
A0A2Z4AGB8_9BACT
250
0
26793
TrEMBL
-
A0A6J5ALD3_9BURK
254
0
26654
TrEMBL
-
A0A2N7W562_9BURK
152
0
15617
TrEMBL
-
A0A6S7B1V2_9BURK
254
0
26824
TrEMBL
-
A0A5S9PXA0_9GAMM
472
0
51655
TrEMBL
-
A0A6G8NZH4_9BURK
252
0
25988
TrEMBL
-
A0A6J5H0Y9_9BURK
258
0
26901
TrEMBL
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A0A204EM44_ACIBA
261
0
28099
TrEMBL
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A0A5E7VQK2_PSEFL
254
0
26678
TrEMBL
-
A0A6S7CXS4_9BURK
252
0
26681
TrEMBL
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A0A5E7I8W4_PSEFL
252
0
26498
TrEMBL
-
A0A6J5G0T2_9BURK
254
0
27076
TrEMBL
-
A0A6J5EIT4_9BURK
271
0
28462
TrEMBL
-
A0A5E6XUF4_PSEFL
254
0
26694
TrEMBL
-
A0A6J5DGE3_9BURK
252
0
26336
TrEMBL
-
A0A5M9IP22_9PSED
256
0
26908
TrEMBL
-
A0A6J5FT59_9BURK
252
0
26130
TrEMBL
-
A0A5E6VIL9_PSEFL
254
0
26794
TrEMBL
-
A0A6M8MRS2_9PSED
254
0
26870
TrEMBL
-
A0A2N7WIX2_9BURK
258
0
27330
TrEMBL
-
A0A6J5FR97_9BURK
254
0
26868
TrEMBL
-
A0A6J5C6Z6_9BURK
258
0
26858
TrEMBL
-
A0A5E7LP54_PSEFL
252
0
26422
TrEMBL
-
A0A5S9N612_9GAMM
286
0
30331
TrEMBL
-
A0A6H2H798_9BURK
255
1
26787
TrEMBL
-
A0A5E6X8V2_PSEFL
254
0
26629
TrEMBL
-
A0A6L5BZ84_9PSED
252
0
26535
TrEMBL
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A0A5E6NXY4_PSEFL
254
0
26865
TrEMBL
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native enzyme partially, separation from 7beta-hydroxysteroid dehydrogenase, EC 1.1.1.201, by hydrophobic interaction chromatography
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gene Rumgna_00694, DNA and amino acid sequence determination and analysis, encoded in a three-gene cluster that does not include any additional putative bile acid-metabolic genes, recombinant overvexpression in Escherichia coli
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Chen, M.; Drury, J.E.; Christianson, D.W.; Penning, T.M.
Conversion of human steroid 5beta-reductase (AKR1D1) into 3beta-hydroxysteroid dehydrogenase by single point mutation E120H: example of perfect enzyme engineering
J. Biol. Chem.
287
16609-16622
2012
Ruminococcus sp., Ruminococcus sp. PO1-3
brenda
Devlin, A.; Fischbach, M.
A biosynthetic pathway for a prominent class of microbiota-derived bile acids
Nat. Chem. Biol.
11
685-690
2015
[Ruminococcus] gnavus (A7AZH2), [Ruminococcus] gnavus ATCC 29149 (A7AZH2)
brenda
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