Information on EC 1.1.1.392 - 3alpha-hydroxycholanate dehydrogenase (NADP+)

for references in articles please use BRENDA:EC1.1.1.392
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The enzyme appears in viruses and cellular organisms

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EC NUMBER
COMMENTARY hide
1.1.1.392
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RECOMMENDED NAME
GeneOntology No.
3alpha-hydroxycholanate dehydrogenase (NADP+)
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REACTION
REACTION DIAGRAM
COMMENTARY hide
ORGANISM
UNIPROT
LITERATURE
lithocholate + NADP+ = 3-oxo-5beta-cholan-24-oate + NADPH + H+
show the reaction diagram
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PATHWAY
BRENDA Link
KEGG Link
MetaCyc Link
iso-bile acids biosynthesis II
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Secondary bile acid biosynthesis
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SYSTEMATIC NAME
IUBMB Comments
lithocholate:NADP+ 3-oxidoreductase
This bacterial enzyme is involved in the modification of secondary bile acids to form 3beta-bile acids (also known as iso-bile acids) via a 3-oxo intermediate. The enzyme catalyses a reversible reaction in vitro. Also acts on related bile acids. cf. EC 1.1.1.52, 3alpha-hydroxycholanate dehydrogenase (NAD+).
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ORGANISM
COMMENTARY hide
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
gene Elen_0690
UniProt
Manually annotated by BRENDA team
gene Elen_0690
UniProt
Manually annotated by BRENDA team
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
metabolism
physiological function
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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
show the reaction diagram
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NATURAL SUBSTRATES
NATURAL PRODUCTS
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
12alpha-hydroxy-3-oxo-5beta-cholan-24-oic acid + NADH + H+
3alpha,12alpha-dihydroxy-5beta-cholan-24-oic acid + NAD+
show the reaction diagram
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1280
12alpha-Hydroxy-3-oxo-5beta-cholan-24-oic acid
pH 7.0, 37°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4.85
12alpha-Hydroxy-3-oxo-5beta-cholan-24-oic acid
pH 7.0, 37°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
gene Elen_0690, DNA and amino acid sequence determination and analysis, recombinant overvexpression in Escherichia coli
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LINKS TO OTHER DATABASES (specific for EC-Number 1.1.1.392)
ExplorEnz
ExPASy
KEGG
MetaCyc
SABIO-RK
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
InterPro (database of protein families, domains and functional sites)